FM™ 2-10 (N-(3-Triethylammoniumpropyl)-4-(4-(Diethylamino)styryl)Pyridinium Dibromide)
FM&trade; 2-10 (<i>N</i>-(3-Triethylammoniumpropyl)-4-(4-(Diethylamino)styryl)Pyridinium Dibromide)
Invitrogen™

FM™ 2-10 (N-(3-Triethylammoniumpropyl)-4-(4-(Diethylamino)styryl)Pyridinium Dibromide)

FM™ 2-10メンブレンプローブは、活発に発火しているニューロンを同定し、活動依存性の小胞サイクリングのメカニズムを研究するための優れた試薬です。この水溶性色素は細胞に無毒で、水性培地では実質的に非蛍光であるため、細胞膜の外葉に挿入して、強い蛍光を生じさせると考えられています。神経伝達物質を活発に放出しているニューロンでは、再生されたシナプス小胞内で色素が取り込まれ詳細を見る
製品番号(カタログ番号)数量
T75085 mg
製品番号(カタログ番号) T7508
価格(JPY)
55,000
Online offer
Ends: 27-Mar-2026
91,700
割引額 36,700 (40%)
5 mg
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数量:
5 mg
FM™ 2-10メンブレンプローブは、活発に発火しているニューロンを同定し、活動依存性の小胞サイクリングのメカニズムを研究するための優れた試薬です。この水溶性色素は細胞に無毒で、水性培地では実質的に非蛍光であるため、細胞膜の外葉に挿入して、強い蛍光を生じさせると考えられています。神経伝達物質を活発に放出しているニューロンでは、再生されたシナプス小胞内で色素が取り込まれ、神経末端が明るく染色されます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
オレンジ
検出法蛍光
使用対象 (装置)蛍光顕微鏡
製品ラインFM
数量5 mg
出荷条件室温
標識タイプFluorescent Dye
製品タイププローブ
SubCellular Localization細胞膜
Unit Size5 mg
組成および保存条件
室温で保存し、光から保護します。

よくあるご質問(FAQ)

I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (27)

引用および参考文献
Abstract
Ba2+ does not support synaptic vesicle retrieval in rat cerebrocortical synaptosomes.
Authors:Cousin MA, Robinson PJ
Journal:Neurosci Lett
PubMed ID:9754790
To investigate whether any specific requirement for extracellular Ca2+ exists in the control synaptic vesicle retrieval, we examined the ability of the divalent cation Ba2+ to substitute for Ca2+ in both vesicle exocytosis and endocytosis. Ba2+ stimulated glutamate release from rat cerebrocortical synaptosomes. Ba2+-evoked release was inhibited by bafilomycin A1, ... More
Frequency dependence of synaptic vesicle exocytosis in aortic baroreceptor neurons and the role of group III mGluRs.
Authors:Pamidimukkala J, Hay M
Journal:Brain Res
PubMed ID:15051525
Synaptic transmission between baroreceptor afferents and the nucleus tractus solitarius (NTS) is essential for reflex regulation of blood pressure. High frequency stimulation of the afferents in vivo leads to a decrease in synaptic strength and is generally attributed to reduction in presynaptic neurotransmitter release. It has been hypothesized that during ... More
Long-term staining of live Merkel cells with FM dyes.
Authors:Fukuda J, Ishimine H, Masaki Y
Journal:Cell Tissue Res
PubMed ID:12658440
'Live Merkel cells in the skin and hair follicles are known to incorporate a fluorescence dye, quinacrine, which has been utilized to identify and dissect the cells for experiments. Quinacrine fluorescence of the cells is, however, quickly lost and quinacrine-stained Merkel cells soon become difficult to identify in tissue culture. ... More
Two mechanisms of synaptic vesicle recycling in rat brain nerve terminals.
Authors:Cousin MA, Robinson PJ
Journal:J Neurochem
PubMed ID:10987846
'KCl and 4-aminopyridine (4-AP) evoke glutamate release from rat brain cortical nerve terminals by voltage clamping or by Na(+) channel-generated repetitive action potentials, respectively. Stimulation by 4-AP but not KCl is largely mediated by protein kinase C (PKC). To determine whether KCl and 4-AP utilise the same mechanism to release ... More
Calcium triggers calcineurin-dependent synaptic vesicle recycling in mammalian nerve terminals.
Authors:Marks B, McMahon HT
Journal:Curr Biol
PubMed ID:9651678
'BACKGROUND: Following exocytosis at the synapse, synaptic vesicle components are recovered by endocytosis. Morphological analysis has suggested that this occurs by a clathrin-mediated pathway, and the GTPase dynamin is thought to be involved in ''pinching off'' endocytosing vesicles. The finding that the calcium-dependent phosphatase calcineurin can dephosphorylate dynamin and two ... More