UltraComp eBeads™ Spectral Unmixing Beads

UltraComp Spectral eBeads are the third generation of UltraComp beads for use in spectral flow cytometry. Fluorochrome-conjugated antibody binds the spherical particles for use as a single-color spectral unmixing control. Bind with mouse, rat, hamster, rabbit, or recombinant human antibodies.

UltraComp Spectral eBeads features include:

• Multispecies compatibility—bind with mouse, rat, hamster, rabbit, or recombinant human antibodies
• Improved fluorophore compatibility
• Low frequency and severity of unmixing errors

UltraComp Spectral eBeads are designed for use in spectral unmixing and compensation with all fluorochromes excited by ultraviolet (355 nm), violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), or red (633-640 nm) lasers. These UltraComp beads have been optimized for use in spectral flow cytometry, where they unmix fluorophores in a manner similar to single color controls on cells.

Each drop of beads contains two populations: a positive population that binds with any mouse, rat, hamster, rabbit, or recombinant human antibody and a negative population that will not bind with antibody. When a fluorochrome-conjugated antibody is added to the beads, both positive and negative populations are observed. This bimodal distribution can be used to set up single-color controls for unmixing as well as compensation in multicolor and high parameter flow cytometry experiments.