Vybrant™ Cell Adhesion Assay Kit
Vybrant™ Cell Adhesion Assay Kit
Invitrogen™

Vybrant™ Cell Adhesion Assay Kit

Vybrant™細胞接着アッセイキットは、さまざまな種類の細胞に対し、細胞-細胞間または細胞-表面間の接着を迅速かつ高感度に測定するアッセイです。このアッセイでは、カルセインAMで標識した細胞を接着させます。未接着細胞を除去した後、カルセイン蛍光を使用して接着細胞の数を計算します詳細を見る
製品番号(カタログ番号)数量
V131811 kit
製品番号(カタログ番号) V13181
価格(JPY)
101,100
1 kit
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数量:
1 kit
Vybrant™細胞接着アッセイキットは、さまざまな種類の細胞に対し、細胞-細胞間または細胞-表面間の接着を迅速かつ高感度に測定するアッセイです。このアッセイでは、カルセインAMで標識した細胞を接着させます。未接着細胞を除去した後、カルセイン蛍光を使用して接着細胞の数を計算します。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
検出法蛍光
染色剤タイプその他の標識または色素
フォーマット96-ウェルプレート
数量1 kit
出荷条件室温
使用対象(アプリケーション)細胞接着アッセイ
使用対象 (装置)マイクロプレートリーダー
製品ラインVybrant
製品タイプ染色
Unit Size1 kit
組成および保存条件
フリーザー(-5℃~-30℃)に保存し、遮光してください。

よくあるご質問(FAQ)

What are the fluorescence excitation/emission maxima for the Calcein AM and SYTOX Green nucleic acid stain provided in the Vybrant Cell Adhesion Assay Kit?


Calcein AM: Ex/Em = 494 nm/517 nm
SYTOX Green nucleic acid stain: Ex/Em = 504 nm/523 nm

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (33)

引用および参考文献
Abstract
Effect of C-reactive protein on gene expression in vascular endothelial cells.
Authors:Wang Q, Zhu X, Xu Q, Ding X, Chen YE, Song Q,
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:15591095
'C-reactive protein (CRP) is significantly associated with the risk of ischemic cardiovascular disease in epidemiological studies. To explore if CRP has a functional role, we investigated its effect on the gene expression profile of vascular endothelial cells. Human vascular endothelial cells (human umbilical vein endothelial cells and human aortic endothelial ... More
Atherogenic lipids induce adhesion of human coronary artery smooth muscle cells to macrophages by up-regulating chemokine CX3CL1 on smooth muscle cells in a TNFalpha-NFkappaB-dependent manner.
Authors:Barlic J, Zhang Y, Murphy PM,
Journal:J Biol Chem
PubMed ID:17456471
'Recent genetic evidence has implicated the adhesive chemokine CX3CL1 and its leukocyte receptor CX3CR1 in atherosclerosis. We previously proposed a mechanism involving foam cell anchorage to vascular smooth muscle cells because: 1) CX3CL1 and CX3CR1 are expressed by both cell types in mouse and human atherosclerotic lesions; 2) foam cells ... More
ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells.
Authors:Li X, Krawetz R, Liu S, Meng G, Rancourt DE,
Journal:Hum Reprod
PubMed ID:19056770
'Efficient slow freezing protocols within serum-free and feeder-free culture systems are crucial for the clinical application of human embryonic stem (hES) cells. Frequently, however, hES cells must be cryopreserved as clumps when using conventional slow freezing protocols, leading to lower survival rates during freeze-thaw and limiting their recovery and growth ... More
CXCL16 signals via Gi, phosphatidylinositol 3-kinase, Akt, I kappa B kinase, and nuclear factor-kappa B and induces cell-cell adhesion and aortic smooth muscle cell proliferation.
Authors:Chandrasekar B, Bysani S, Mummidi S,
Journal:J Biol Chem
PubMed ID:14625285
'CXCL16, a recently discovered transmembrane chemokine, is expressed in human aortic smooth muscle cell (ASMC). It facilitates uptake of low density lipoproteins by macrophages, resulting in foam cell formation. However, it is not known whether ASMC express CXCR6, the receptor for CXCL16, or whether CXCL16 affects ASMC biology. To dissect ... More
Targeting PKC in multiple myeloma: in vitro and in vivo effects of the novel, orally available small-molecule inhibitor enzastaurin (LY317615.HCl).
Authors:Podar K, Raab MS, Zhang J, McMillin D, Breitkreutz I, Tai YT, Lin BK, Munshi N, Hideshima T, Chauhan D, Anderson KC,
Journal:Blood
PubMed ID:17023575
'In multiple myeloma (MM) protein kinase C (PKC) signaling pathways have been implicated in cell proliferation, survival, and migration. Here we investigated the novel, orally available PKC-inhibitor enzastaurin for its anti-MM activity. Enzastaurin specifically inhibits phorbol ester-induced activation of PKC isoforms, as well as phosphorylation of downstream signaling molecules MARCKS ... More