Vybrant Apoptosis Assay Kit #5、またはChromatin Condensation/Dead Cell Apoptosis Kit with Hoechst 33342 and PI for Flow Cytometryは、アポトーシス細胞におけるクロマチンのコンパクト状態の蛍光検出をベースにした迅速かつ便利なアポトーシス用アッセイとして使用できます。このキットには、すぐに使用できる溶液として、青色蛍光Hoechst 33342色素(アポトーシスを起こしていない細胞のクロマチンよりもアポトーシスを起こしている細胞の凝縮クロマチンを大幅に明るく染色する)のほか、赤色蛍光ヨウ化プロピジウム色素(死細胞を染色する)が含まれています。
What are the fluorescence excitation/emission maxima for the dyes in the Membrane Permeability/Dead Cell Apoptosis Kit with YO-PRO-1 and PI for Flow Cytometry aka Vybrant Apoptosis Assay Kit #4, YO-PRO-1/Propidium Iodide)?
YO-PRO-1: 491/509 nm, bound to DNA
Propidium iodide: 535/617 nm, bound to DNA
What is the relative time-frame of apoptosis progression, and what products can I use to detect the different apoptotic events?
See Table 1 in this article (http://www.thermofisher.com/content/dam/LifeTech/migration/en/filelibrary/support/bioprobes/bioprobes-65.par.61751.file.dat/bioprobes-65-cellevent.pdf) where a Jurkat model system was induced with 10 µM camptothecin for time periods of 0 to 4 hours. It is important to note that these results were studied using a single cell type and induction system; results may differ for other experimental systems. Increased membrane permeability in apoptotic cells can be discriminated from dead cells using YO-PRO-1 dye in combination with propidium iodide or the SYTOX dead cell indicators. Other mid-apoptotic events are increased ROS production (detected with CellROX reagents, H2DCFDA), changes in cellular pH (BCECF, SNARF-1) and calcium release (Fluo-4, Fura-2, Indo-1). No single parameter defines apoptosis under any condition, so it is best to employ a multi-parametric approach when studying apoptosis.
Contribution of membrane localization to the apoptotic activity of PUMA.
Authors:Yee KS, Vousden KH,
Journal:Apoptosis
PubMed ID:17968660
'The BH3-only protein PUMA plays an important role in the activation of apoptosis in response to p53. In different studies, PUMA has been described to function by either directly activating the pro-apoptotic proteins Bax and Bak, or by neutralizing anti-apoptotic members of the Bcl2 family. We have examined the contribution ... More
Release of hypoacetylated and trimethylated histone H4 is an epigenetic marker of early apoptosis.
Authors:Boix-Chornet M, Fraga MF, Villar-Garea A, Caballero R, Espada J, Nuñez A, Casado J, Largo C, Casal JI, Cigudosa JC, Franco L, Esteller M, Ballestar E
Journal:J Biol Chem
PubMed ID:16531610
'Nuclear events such as chromatin condensation, DNA cleavage at internucleosomal sites, and histone release from chromatin are recognized as hallmarks of apoptosis. However, there is no complete understanding of the molecular events underlying these changes. It is likely that epigenetic changes such as DNA methylation and histone modifications that are ... More
Chlorophenols and chlorocatechols induce apoptosis in human lymphocytes (in vitro).
Authors:Michalowicz J, Sicinska P,
Journal:Toxicol Lett
PubMed ID:19766705
In this work the effect of 2,4,5-trichlorophenol (2,4,5-TCP), pentachlorophenol (PCP), 4,6-dichloroguaiacol (4,6-DCG), tetrachloroguaiacol (TeCG), 4,5-dichlorocatechol (4,5-DCC) and tetrachlorocatechol (TeCC) on the induction of apoptosis in human peripheral blood lymphocytes was examined. The analysis of the changes in mitochondrial transmembrane potential (DeltaPsi(m)) was performed using JC-9 fluorescent probe. It was noted ... More
Comparison of apoptosis and mortality measurements in peripheral blood mononuclear cells (PBMCs) using multiple methods.
Death through apoptosis is the main process by which aged cells that have lost their function are eliminated. Apoptotic cells are usually detected microscopically by changes in their morphology. However, determination of early apoptotic events is important for in vitro (and ex vivo) studies. The main objective of the present ... More
Relocalization of STIM1 for activation of store-operated Ca(2+) entry is determined by the depletion of subplasma membrane endoplasmic reticulum Ca(2+) store.
Authors:Ong HL, Liu X, Tsaneva-Atanasova K, Singh BB, Bandyopadhyay BC, Swaim WD, Russell JT, Hegde RS, Sherman A, Ambudkar IS,
Journal:J Biol Chem
PubMed ID:17298947
STIM1 (stromal interacting molecule 1), an endoplasmic reticulum (ER) protein that controls store-operated Ca(2+) entry (SOCE), redistributes into punctae at the cell periphery after store depletion. This redistribution is suggested to have a causal role in activation of SOCE. However, whether peripheral STIM1 punctae that are involved in regulation of ... More