pLenti6/V5-DEST™ Gateway™ Vector

Citations & References (4)

Invitrogen™

pLenti6/V5-DEST™ Gateway™ Vector

製品番号（カタログ番号）	数量
V49610	6 μg

製品番号（カタログ番号） V49610

価格（JPY）

436,400

お問い合わせください ›

pLenti6⁄V5-DEST™ Gateway™ Vectorは、哺乳類細胞の分裂および非分裂におけるターゲット遺伝子のレンチウイルスベース発現用のGateway適応型ViraPower™レンチウイルス発現ベクターです。ベクターには、哺乳類細胞でのターゲット遺伝子の構成的な発現、および安定的な選択のためのブラストサイジン選択マーカーのためのCMVプロモーターがあります。

利点
• 哺乳類細胞の分裂および非分裂におけるターゲット遺伝子のレンチウイルスベース発現

主な特長
• 柔軟で多用途なGateway™組換えクローニングテクノロジー
• CMVプロモーターでの構成的な高レベルでの発現
• 安定的な選択のためのブラストサイジン選択マーカー
•迅速な検出のためのC末端V5タグ

キットには、次のものが含まれています。
• pLenti6⁄V5-DEST™ Gateway™ Vector
• One Shot™ Stbl3™ケミカルコンピテント大腸菌（C7373-03）

関連するSKU
• pLenti6⁄UbC⁄V5-DEST™ Gateway™ Vector（V49910）
• pLenti4⁄V5-DEST™ Gateway™ Vector（V49810）
• pLenti6⁄V5 Directional TOPO™ クローニングキット（K4955-10)
• ViraPower™ Lentiviral Directional TOPO™ Expression Kit（K495000）
• ViraPower™ Lentiviral Gateway™ Expression Kit（K4960-00）
• ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit（K5330-00）

研究目的のみに使用できます。あらゆる治療もしくは診断用には使用できません。

研究用にのみ使用できます。診断用には使用いただけません。

構成または誘導システム構造的

供給タイプレンチウイルス

使用対象（アプリケーション）ウイルス発現

製品タイプレンチウイルス発現ベクター

数量6 μg

選択剤（真核生物）ブラストサイジン

ベクターpDEST、pLenti

クローニング法Gateway™

製品ラインGateway、ViraPower, ViraPower

プロモーターCMV

タンパク質タグV5エピトープタグ

Unit Size6 µg

組成および保存条件

ViraPower™ Lentiviral Gateway™ Vectorsは、–20℃で保存し、次のものが含まれます：

pLenti6⁄V5-DEST™：6µg（40µLの150 ng/µLベクター、10 mM
Tris-HCl内、1mM EDTA、pH 8.0）

pLenti6⁄V5-GW⁄lacZコントロール：10µg
（20µLの 0.5µg⁄µlベクター、10 mM
Tris-HCl、1mM EDTA、pH 8.0）

One Shot™ Stbl3™ケミカルコンピテント大腸菌。–80℃で保管してください。

よくあるご質問（FAQ）

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all pLenti6/V5-DEST™ Gateway™ Vector FAQs

引用および参考文献 (4)

引用および参考文献

Abstract

The membrane anchor R7BP controls the proteolytic stability of the striatal specific RGS protein, RGS9-2.

Authors:Anderson GR, Semenov A, Song JH, Martemyanov KA,

Journal:J Biol Chem

PubMed ID:17158100

'A member of the RGS (regulators of G protein signaling) family, RGS9-2 is a critical regulator of G protein signaling pathways that control locomotion and reward signaling in the brain. RGS9-2 is specifically expressed in striatal neurons where it forms complexes with its newly discovered partner, R7BP (R7 family binding ... More

West Nile virus discriminates between DC-SIGN and DC-SIGNR for cellular attachment and infection.

Authors:Davis CW, Nguyen HY, Hanna SL, Sánchez MD, Doms RW, Pierson TC,

Journal:J Virol

PubMed ID:16415006

'The C-type lectins DC-SIGN and DC-SIGNR bind mannose-rich glycans with high affinity. In vitro, cells expressing these attachment factors efficiently capture, and are infected by, a diverse array of appropriately glycosylated pathogens, including dengue virus. In this study, we investigated whether these lectins could enhance cellular infection by West Nile ... More

Tumorigenesis suppressor Pdcd4 down-regulates mitogen-activated protein kinase kinase kinase kinase 1 expression to suppress colon carcinoma cell invasion.

Authors:Yang HS, Matthews CP, Clair T, Wang Q, Baker AR, Li CC, Tan TH, Colburn NH,

Journal:Mol Cell Biol

PubMed ID:16449643

Programmed cell death 4 (Pdcd4) suppresses neoplastic transformation by inhibiting the activation of c-Jun and consequently AP-1-dependent transcription. We report that Pdcd4 blocks c-Jun activation by inhibiting the expression of mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1)/hematopoietic progenitor kinase 1, a kinase upstream of Jun N-terminal kinase (JNK). cDNA ... More

Isolation of cell lines that show novel, murine leukemia virus-specific blocks to early steps of retroviral replication.

Authors:Bruce JW, Bradley KA, Ahlquist P, Young JA,

Journal:J Virol

PubMed ID:16188999

In order to identify cellular proteins required for early stages of retroviral replication, a high volume screening with mammalian somatic cells was performed. Ten pools of chemically mutagenized Chinese hamster ovary (CHO-K1) cells were challenged with a murine leukemia virus (MLV) vector pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G), ... More