WesternBreeze™ Wash Solution (16X)

WesternBreeze®洗浄溶液（16X）は、ニトロセルロース（NC）およびポリフッ化ビニリデン（PVDF）膜でバックグラウンドと非特異的結合を最小限に抑えるために、界面活性剤を含む濃縮バッファー生理的食塩水を最適化した溶液です。ミニブロット20回分に十分な試薬です詳細を見る

製品番号（カタログ番号）	数量
WB7003	2 x 100 mL

製品番号（カタログ番号） WB7003

価格（JPY）

15,700

Online offer

Ends: 27-Mar-2026

26,200

割引額 10,500 (40%)

2 x 100 mL

お問い合わせください ›

2 x 100 mL

WesternBreeze®洗浄溶液（16X）は、ニトロセルロース（NC）およびポリフッ化ビニリデン（PVDF）膜でバックグラウンドと非特異的結合を最小限に抑えるために、界面活性剤を含む濃縮バッファー生理的食塩水を最適化した溶液です。ミニブロット20回分に十分な試薬です。

研究用にのみ使用できます。診断用には使用いただけません。

バッファーブロッティングバッファー

数量2 x 100 mL

Membrane CompatibilityNitrocellulose, PVDF

製品ラインWesternBreeze

製品タイプ洗浄液

Unit Size2 x 100 mL

組成および保存条件

100 ml抗体洗浄溶液（16X）2個

+4℃で保存してください。

よくあるご質問（FAQ）

Why is the actual band size on a western blot different from the predicted size of the protein?

Western blotting is based on the separation of proteins by their size on a gel. However, migration of proteins through the gel matrix is also affected by other factors, which may cause the observed band size to be different from the predicted size.

Common causes are:
-Post-translational modification; for example phosphorylation and glycosylation increase the size of the protein
-Post-translation cleavage; many proteins are synthesized as precursor proteins, and then cleaved to give the active form
-Multimers, for example dimerization of a protein. This is usually prevented under reducing conditions, although strong interactions can result in the appearance of higher bands
-Splice variants; alternative splicing may result in different sized proteins being produced from the same gene
-Relative charge; the composition of amino acids (charged vs. non-charged)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Can I purchase the Blocker/Diluent and Antibody Wash solutions from the WesternBreeze Chemiluminescent Detection kits as standalone products?

Yes, you may purchase them as standalone products using the Cat. Nos. listed below:

- Cat. No. WB7001 (Blocker/Diluent A)
- Cat. No. WB7002 (Blocker/Diluent B)
- Cat. No. WB7050 (Combo pack containing Blocker/Diluents A & B)
- Cat. No. WB7003 (Antibody Wash)

Note: Cat. Nos. WB7001 and WB7002 may be ordered by clicking the Quick Order button located at the top right of any page on our website.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I purchase the Blocker/Diluent and Antibody Wash solutions from the WesternBreeze Chromogenic Detection kits as standalone products?

Yes, you may purchase them as standalone products using the Cat. Nos. listed below:

- Cat. No. WB7003 (Antibody Wash)
- Cat. No. WB7001 (Blocker/Diluent A)
- Cat. No. WB7002 (Blocker/Diluent B)
- Cat. No. WB7050 (Combo pack containing Blocker/Diluents A & B)

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.