NuPAGE™ Bis-Tris Midi Protein Gels, 4 to 12%, 1.0 mm, WedgeWell™ format

Invitrogen NuPAGE Bis-Tris WedgeWell format protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important.

Product features

• Better protein integrity—optimized sample preparation process preserves your proteins
• Faster run times—get separation of your proteins in as little as 30 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for 12 months at room temperature
• Wedge-shaped wells—for easier sample loading and up to two times higher sample volume

Choose the right NuPAGE Bis-Tris gel for your protein separation

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer (NP0007) and NuPAGE Sample Reducing Agent (NP0004). NuPAGE Antioxidant (NP0005) can be added to the running buffer to maintain the reduced state of the proteins during the run allowing for maximum band sharpness if desired. The gels can be run using NuPAGE MES SDS Running Buffer (NP0002) to better resolve small proteins with a faster run time or NuPAGE MOPS SDS Running Buffer (NP000102) to resolve medium- to large-size proteins.

NuPAGE WedgeWell Bis-Tris Midi gels can be run in the SureLock Tandem Midi Gel Tank (STM1001) or XCell4 SureLock Midi-Cell (WR0100).

For transfer of proteins to a membrane, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 3 Gel Transfer Device (IB31001). Alternatively, traditional wet transfer can be performed using the SureLock Tandem Midi Blot Module (STM2001) using NuPAGE Transfer buffer (NP00061).

Convenient to use

NuPAGE Bis-Tris WedgeWell format gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.