Shop All Microbial Isolation & Concentration Kits

Dynabeads™ anti-Cryptosporidium (Applied Biosystems™)

For the selective enrichment of Cryptosporidium oocysts directly from water sample concentrates. Performance tested: Validated IMS in the US Environment Protection Agency's Method 1622 and by UK DETR Water Supply Regulation 1999 SI No 1524.

Benefits and features

Improved oocyst recovery

• Simple procedure
• Reduced background debris aids detection by microscopy
• One clean 8-10 mm slide well per sample
• Saves up to 6 hours of hands-on processing time

This Product Contains: Dynabeads® coated with anti-Cryptosporidium antibodies and SL buffer

Pathatrix™ 10 Pooling Salmonella spp. Kit (Applied Biosystems™)

The Pathatrix® 10 Pooling Salmonella spp. Kit, when used with the Pathatrix® Auto Instrument, permits the concentration and isolation of Salmonella spp. bacteria from pre-enriched food and environmental samples for downstream detection by PCR, immunoassay or culture. This kit format supports pooling of 10 enriched samples into a single test, which can reduce downstream testing costs by up to 90%.

When used with the Pathatrix® Auto Instrument, the Pathatrix® 10 Pooling Salmonella spp. Kit:
• Helps save money – supports pooling protocols that save up to 90% on downstream detection costs
• Minimizes false positive and indeterminate rates – isolates specific bacteria from background flora and PCR inhibitors
• Reduces time-to-results – concentrates target organisms, so less enrichment time is required

Each kit includes one vial of antibody-coated beads and sufficient numbers of sterile, single-use plastic consumables for 50 pooled tests (500 individual, enriched samples).

Pathatrix™ Salmonella spp. Kit - Same Day (Applied Biosystems™)

The Pathatrix® Salmonella spp. Kit - Same Day, when used with the Pathatrix® Auto Instrument, facilitates the concentration and isolation of Salmonella bacteria from pre-enriched food and environmental samples for downstream detection by PCR, immunoassay or culture. The same day format supports protocols that require pre-enrichment for 8 hours or less prior to re-circulation.

When used with the Pathatrix® Auto Instrument, the Pathatrix® Salmonella spp. Kit - Same Day:
• Reduces time-to-results – concentrates target organisms, so less enrichment time is required
• Minimizes false positive and indeterminate rates – isolates specific bacteria from background flora and PCR inhibitors
• Provides added confidence – workflows validated by the AOAC Research Institute

Each kit includes one vial of antibody-coated beads and sufficient numbers of sterile, single-use plastic consumables for 50 samples.

Dynabeads™ MAX EPEC/VTEC O45 Kit (Applied Biosystems™)

The Dynabeads MAX EPEC/VTEC O45 Kit allows for the rapid, selective concentration of E. coli O45 serogroup bacteria in food and environmental samples to improve downstream detection via PCR or culture methods.

• Improve detection sensitivity in PCR sample analysis
• Reduce background in selective agar detection
• Perform the method manually or automate using the Thermo Scientific KingFisher mL Food Protection Purification System System

Improve Sensitivity and Reduce Background
Highly specific antibodies attached to paramagnetic beads are incubated with an aliquot of pre-enriched sample. The beads bind and concentrate the target bacteria from non-target organisms. Washing steps help limit the carryover of inhibitors and matrix-associated particulates. These steps combine to improve the sensitivity of PCR-based detection while reducing background for plating methods.

Manual or Automated Processing
The immunomagnetic separation (IMS) steps can be performed manually with magnets and mixers or automated using the Thermo Scientific KingFisher mL Food Protection Purification System.

Sensitivity
Following the Dynabeads MAX EPEC/VTEC O45 protocol, the presence or absence of one viable E. coli O45 can be determined in the sample sizes described if this one E. coli cell is able to replicate and is not masked by resident background flora.

Dynabeads™ EPEC/VTEC O103 (Applied Biosystems™)

For the rapid selective concentration of E.coli 0103. Method can be performed manually or can be automated by using a Thermo Scientific KingFisher mL Food Protection Purification System.

This Product Contains: Dynabeads coated with anti-E.coli 0103 antibodies

Sensitivity: Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre enrichment

Dynabeads™ EPEC/VTEC O26 (Applied Biosystems™)

For the rapid selective concentration of E.coli 026. Method can be performed manually or can be automated by using a Thermo Scientific KingFisher mL Food Protection Purification System.

This Product Contains: Dynabeads coated with anti-E.coli O26 antibodies

Sensitivity: Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre enrichment

Pathatrix™ Cronobacter spp. 5-Pooling Kit (Applied Biosystems™)

The Pathatrix® Cronobacter spp. 5-Pooling Kit, when used with the Pathatrix® Auto Instrument, facilitates the concentration and isolation of Cronobacter spp. bacteria from pre-enriched food and environmental samples for downstream detection by PCR, immunoassay, or culture plating. This kit format supports pooling of up to 5 enriched samples into a single test, which can help reduce downstream testing costs by up to 80%.

When used with the Pathatrix® Auto Instrument, the Pathatrix® Cronobacter spp. 5-Pooling Kit helps:
• Save up to 80% on downstream detection costs via pooling protocols
• Reduce false positives and indeterminates by separating target bacteria from background flora and inhibitors via a cationic paramagnetic bead
• Reduce time-to-results: obtain presumptive results typically in 32 hours via selective agar

Reduce Cost and Effort
The detection test itself is the most costly component of pathogen testing. By pooling enriched samples together as a collective sample, the number of detection tests is drastically reduced, thereby reducing the overall cost and effort associated with the detection workflow.

Kit Sensitivity
The procedure allows you to detect as few as 1-10 cfu from a 25 g food sample. The limitation of the Pathatrix® Cronobacter spp. 5‐Pooling Kit is in the ability of the target to reproduce in the enrichment medium, be captured by the magnet, and subsequently be isolated on selective agar plates.

Dynabeads™ MAX EPEC/VTEC O121 Kit (Applied Biosystems™)

The Dynabeads MAX EPEC/VTEC O121 Kit allows for the rapid, selective concentration of E. coli O121 serogroup bacteria in food and environmental samples to improve downstream detection via PCR or culture methods.

• Improve detection sensitivity in PCR sample analysis
• Reduce background in selective agar detection
• Perform the method manually or automate using the Thermo Scientific KingFisher mL Food Protection Purification System

Improve Sensitivity and Reduce Background
Highly specific antibodies attached to paramagnetic beads are incubated with an aliquot of pre-enriched sample. The beads bind and concentrate the target bacteria from non-target organisms. Washing steps help limit the carryover of inhibitors and matrix-associated particulates. These steps combine to improve the sensitivity of PCR-based detection while reducing background for plating methods.

Manual or Automated Processing
The immunomagnetic separation (IMS) steps can be performed manually with magnets and mixers or automated using the Thermo Scientific KingFisher mL Food Protection Purification System.

Sensitivity
Following the Dynabeads MAX EPEC/VTEC O121 protocol, the presence or absence of one viable E. coli O121 can be determined in the sample sizes described if this one E. coli cell is able to replicate and is not masked by resident background flora.

NOTE: Lot 1375851 of this product contains only 1 vial of paramagnetic beads (1 mL each) sufficient for 100 reactions. This batch follows a slightly modified protocol (see use instructions in document part number MAN0007304 Revision 1.0).

Dynabeads™ MAX E. coli O157 Kit (Applied Biosystems™)

Dynabeads MAX E.Coli O157 provides a powerful sample preparation method to improve PCR detection and quantification of E.Coli O157:H7 in food, feces, water and other environmental samples.
Based on well known and widely used immunomagnetic separation (IMS) technology, Dynabeads MAX E.Coli O157 provides a selective E.Coli O157:H7 capture step that delivers significant improvements in PCR analytical process time, as well as the sensitivity and consistency of results. With Dynabeads MAX E.Coli O157, you'll:

• Perform high throughput, rapid, and effective E.Coli O157:H7 sample preparation
• Significantly shorten sample pre-enrichment time
• Improve detection sensitivity in PCR sample analysis
• Significantly reduce overall analysis time
• Can be carried out manually or automated using the Thermo Scientific KingFisher mL Food Protection Purification System.

Pathatrix™ Cronobacter spp. Kit, same day (Applied Biosystems™)

The Pathatrix® Cronobacter spp. Kit – Same Day, when used with the Pathatrix® Auto Instrument, facilitates the concentration of Cronobacter bacteria from pre-enriched food and environmental samples for downstream detection by PCR, immunoassay or culture. The kit is optimized for use with protocols that require 8 or fewer hours of pre-enrichment time.

Each kit includes one vial of antibody-coated beads and sufficient numbers of sterile, single-use plastic consumables for 50 samples.

Pathatrix™ 5 Pooling Salmonella spp. Kit (Applied Biosystems™)

The Pathatrix® 5 Pooling Salmonella spp. Kit, when used with the Pathatrix® Auto Instrument, permits the concentration and isolation of Salmonella spp. bacteria from pre-enriched food and environmental samples for downstream detection by PCR, immunoassay or culture. This kit format supports pooling of 5 enriched samples into a single test, which can reduce downstream testing costs by up to 80%.

When used with the Pathatrix® Auto Instrument, the Pathatrix® 5 Pooling Salmonella spp. Kit:
• Helps save money – supports pooling protocols that save up to 80% on downstream detection costs
• Minimizes false positive and indeterminate rates – isolates specific bacteria from background flora and PCR inhibitors
• Reduces time-to-results – concentrates target organisms, so less enrichment time is required

Each kit includes one vial of antibody-coated beads and sufficient numbers of sterile, single-use plastic consumables for 50 pooled tests (250 individual, enriched samples).

Dynabeads™ anti-Listeria (Applied Biosystems™)

For the selective enrichment of Listeria directly from pre-enrichment samples using ImmunoMagnetic Separation. Method can be performed manually or can be automated by using a Thermo Scientific KingFisher mL Food Protection Purification System.

Benefits and features

Saves processing and confirmation time

• Increases sensitivity and positive isolation rate
• Easy IMS protocol - 30 minutes from start to finish
• Shelf stable reagent and low start up costs

This Product Contains: Dynabeads coated with anti-Listeria antibodies

Dynabeads™ EPEC/VTEC O145 (Applied Biosystems™)

For the rapid selective concentration of E.coli O145. Method can be performed manually or can be automated by using a BeadRetriever.

This Product Contains: Dynabeads coated with anti-E.coli O145 antibodies

Sensitivity: Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre enrichment

Pathatrix™ Listeria spp. Kit (Applied Biosystems™)

The Pathatrix® Listeria spp. Kit, when used with the Pathatrix® Auto Instrument, facilitates the concentration and isolation of Listeria bacteria from pre-enriched food and environmental samples for downstream detection of Listeria spp. or Listeria monocytogenes by PCR, immunoassay or culture.

When used with the Pathatrix® Auto Instrument, the Pathatrix® Listeria spp. Kit:
• Reduces time-to-results – concentrates target organisms, so less enrichment time is required
• Minimizes false positive and indeterminate rates – isolates specific bacteria from background flora and PCR inhibitors
• Provides added confidence – workflows validated by the AOAC Research Institute

Dynabeads™ GC-Combo (Applied Biosystems™)

For the selective enrichment of Giardia cysts and Cryptosporidium oocysts directly from water sample concentrates.

Performance tested: Validated IMS in the US Environment Protection Agency's Method 1623.

Benefits and features

• Significant improved recovery of Cryptosporidium & Giardia
• Simple procedure
• Reduced background debris aids detection by microscopy
• One clean 9mm slide well per sample
• Saves hours of hands-on processing time

This Product Contains: Dynabeads® coated with either anti-Cryptosporidium or anti-Giardia antibodies and SL buffer