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Fluoroskan™ FL Microplate Fluorometer and Luminometer, two dispensers - FAQs

View additional product information for Fluoroskan™ FL Microplate Fluorometer and Luminometer, two dispensers - FAQs (5200222)

11 product FAQs found

Can I measure fluorescence and luminescence from the same well on the Fluoroskan FL Microplate Fluorometer and Luminometer?

Yes, this can be done by adding fluorescence and luminescence measurement steps to the Protocol section of the SkanIt Software session.

How many filters can be installed in the Fluoroskan FL Microplate Fluorometer and Luminometer?

The Fluoroskan FL Microplate Fluorometer excitation filter wheel can hold a maximum of eight filters and the emission wheel can hold a maximum of six filters.

When is the light shield needed with the Fluoroskan FL Microplate Fluorometer and Luminometer?

The light shield is needed for luminometric measurements and does not interfere with fluorometric measurements. The light shield should be used with 96- and 384-well plates.

For plates higher than 15 mm, the light shield must be removed.

Can the light shield be removed from the Fluoroskan FL Microplate Fluorometer and Luminometer while carrying out fluorometric measurements?

Yes, the light shield can be removed as it is is only needed with luminometric measurements.

Does the Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer have different beams for different plate types?

The Fluoroskan Microplate Fluorometer and Fluoroskan FL Microplate Fluorometer and Luminometer have two beam diameter settings. The normal beam with a diameter of 3 mm is generally recommended for 96-well plates and larger wells. The small beam with a diameter of 1.5 mm is recommended for 384-well plates.

How do the fluorescence reference chips, which are installed into the Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer plate carrier work?

The fluorescent chips are installed into the unit to keep the instrument signal level stable. When a filter pair is validated, the instrument measures all four chips in the plate carrier and then automatically selects the most suitable chip. The chip measurement value is stored in the instrument memory. When the measurement is started, autocalibration is performed. The instrument measures this chip during the autocalibration process and compares it with the value in memory. A correction factor is calculated and all further measurement values are multiplied by this factor.

Which filters ship standard with Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer?

The Fluoroksan Microplate Fluorometer and Fluoroskan FL Microplate Fluorometer and Luminometer ship with two filter pairs:
- Ex 355 and Em 460
- Ex 485 and Em 538
For a complete list of available filters, contact your local Thermo Scientific microplate instrumentation technical support.

Can my Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer perform spectral scans?

No, they cannot perform spectral scans. For scanning applications, see Varioskan LUX Multimode Microplate Reader information here: https://www.thermofisher.com/us/en/home/life-science/lab-equipment/microplate-instruments/microplate-readers/varioskan-lux-multimode-microplate-reader.html

Can my Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer read time-resolved fluorescence (TRF) or time-resolved FRET (TR-FRET) assays?

No, they cannot read time-resolved fluorescence (TRF/TR-FRET). For these applications, see Varioskan LUX Multimode Microplate Reader information here: https://www.thermofisher.com/us/en/home/life-science/lab-equipment/microplate-instruments/microplate-readers/varioskan-lux-multimode-microplate-reader.html.

Can my Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer read FRET assays?

Yes, they can read any type of fluorescence intensity including FRET assays.

Can my Luminoskan Microplate Luminometer or Fluoroskan FL Microplate Fluorometer and Luminometer read different types of luminescence assays including kinetic, dual luminescence, flash, glow, BRET, BRET2, or NanoBRET assays?

Yes, Luminoskan Microplate Luminometer and Fluoroskan FL Microplate Fluorometer and Luminometer can read any type of luminescence assay. Some will require filters.