New SYBR® Select Master Mix | Performance Benchmarking | Related Products

Performance Benchmarking

SYBR® Select Master Mix outperforms the competition with respect to:

  • Specificity — specific amplification for 100% of assays
  • Brightness — optimum brightness for high performance on most Real-Time PCR instruments
  • Sensitivity — true single copy detection as demonstrated with digital PCR
  • Dynamic range — more tolerant to high input cDNA

Exceptional Specificity

Out of 44 assays and 3 independent replicate reactions only SYBR® Select Master Mix had a single peak in all the melt curves (100% specificity)


Figure 1 - Percentage of assays with Single Peak in Melt Curve

Brightness that is Just Right

SYBR® Select is brighter than the majority of competitor master mixes, but not too bright to upset some Real-Time PCR instruments


Figure 2 – Average delta Rn (relative fluorescence) across 4 dilution points for PGK1 assay

Spectacular Dynamic Range and Linearity

SYBR® Select produces no NTC signal and is more tolerant to high-input DNA


Single Copy Detection using dPCR and gDNA with RNaseP target