Cell Viability and Function Information

Learn about assays and techniques for detecting and monitoring cell health and function by flow cytometry, imaging, high-content analysis and microplate assays. Topics include apoptosis, autophagy, cell counting, proliferation and viability as well as cell cycle, endocytosis, ion indicators and ion channels, oxidative stress and more.

Cell viability and function features

BioProbes article

BioProbes article

Multiplex assays for robust cell health analyses

Here we feature an overview of plate-based viability assays which are a fundamental tool in drug discovery for evaluating the potency of compounds and the sensitivity of cell lines to specific agents.

Application note

Fluorescent viability assays on the Countess II FL Automated Cell Counter

Fluorescent viability assays on the Countess II FL Automated Cell Counter

Assessing cell viability is a key step in daily cell manipulation and is required for accurate and efficient downstream processing. In bright-field microscopy, the most commonly used method is trypan blue staining; however, many fluorescent options also exist and are commonly used in microscopy and flow cytometry applications.


Cell viability and function subtopics


Cell viability and function learning resources

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Type Title Categories
Application note (2011) Apoptosis detection: Apoptosis assays for the Attune Acoustic Focusing Cytometer  Alexa Fluor, apoptosis, Attune/Attune NxT, cell structure-mitochondria, cell structure-plasma membrane, flow cytometer/flow cytometry, fluorescent dyes
Application note (2011) Bacterial analysis using the Attune Acoustic Focusing Cytometer  Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, microbiology, viability
Application note (2011) Detection of human mesenchymal stem cells using the Attune Acoustic Focusing Cytometer  Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, immunophenotyping, stem cell research
Application note (2012) Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening  ArrayScan, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, neuroscience, viability
Application note (2012) Microbiological applications using the Attune Acoustic Focusing Cytometer  Attune/Attune NxT, cell health, flow cytometer/flow cytometry, fluorescent dyes, microbiology, viability
Application note (2013) Multiplexed mitosis and apoptosis analysis  antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, particles
Application note (2014) High content cell cycle screening: Pairing FUCCI technology with Thermo Scientific HCS Studio 2.0 Software and FCS Express Image cytometry  ArrayScan, cancer, cell cycle, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Application note (2014) Oxidative stress measurements made easy: CellROX reagents with simple workflow for high content imaging  ArrayScan, CellInsight, CellROX, high content analysis, oxidative stress
Application note (2014) Elucidating the mechanism of cell death through the use of high content analysis  apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Application note (2015) Guide to setting up hypoxic conditions on the EVOS FL Auto Imaging System with Onstage Incubator EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator, phagocytosis
Application note (2015) Quantitation of proliferating cells with the EVOS FL Auto Imaging System antibodies, antibody labeling, ArrayScan, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, onstage incubator, phagocytosis
Application note (2015) Image tiling and stitching using the EVOS FL Auto Imaging System cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, Imaging sample preparation, ReadyProbes
Application note (2015) Fluorescent viability assays on the Countess II FL Automated Cell Counter automated cell counter, cell counting, Countess, EVOS, fluorescent dyes, ReadyProbes, viability
Application note (2015) Fluorescent apoptosis evaluation on the Countess II FL Automated Cell Counter apoptosis, automated cell counter, cell counting, Countess, EVOS, fluorescent dyes, viability
Application note (2015) Cell division and migration during wound healing visualized on the EVOS FL Auto Imaging System cell health, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging
Application note (2015) Live/dead algal analysis with the Attune NxT Flow Cytometer algae, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, viability
Application note (2015) No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Flow Cytometer antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immune system, immunophenotyping, particles, pH detection
Application note (2017) Flow cytometry analysis of dose response for apoptosis induction apoptosis, Attune/Attune NxT, cancer, flow cytometer/flow cytometry
BioProbes article (2006) Analyzing cell culture health and concentration  apoptosis, cell health, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, microplate reader, particles, viability
BioProbes article (2006) Invitrogen flow cytometry—building choice  cell culture, cell cycle, cell health, cell isolation, cell signaling, flow cytometer/flow cytometry, fluorescent dyes, Gibco, fluorescence microscopy/fluorescence imaging, nanocrystals, protein labeling, Qdot, Zenon
BioProbes article (2006) Products for neurobiology research  enzyme activity detection, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in situhybridization, nanocrystals, protein detection, protein labeling, Qdot, viability, Zenon
BioProbes article (2007) Staining bacterial biofilms: new uses for classic fluorescent dyes  biofilm staining, CellTrace, enzyme activity detection, fluorescent dyes, fluorescence microscopy/fluorescence imaging, microbiology, viability
BioProbes article (2007) Seeing superoxide in a radical new light  cell structure-mitochondria, fluorescent dyes, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress, sample stability
BioProbes article (2007) Looking for apoptosis?  antibodies, apoptosis, colorimetric, ELISA, flow cytometer/flow cytometry,fluorescent dyes, immunocytochemistry (ICC), protein detection, western blotting
BioProbes article (2007) Mitochondria and cardiac disorders  antibodies, antibody labeling, cardiovascular system, cell structure-mitochondria, fluorescent dyes, fluorescence microscopy/fluorescence imaging, oxidative stress, western blotting
BioProbes article (2007) Breakthrough cell proliferation assay for flow cytometry  Alexa Fluor, cell proliferation, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nucleic acid quantitation
BioProbes article (2007) Fluorescent calcium indicators for in vivo imaging of neuronal networks  cell signaling, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in vivo imaging, ion indicators, neuroscience, two-photon microscopy
BioProbes article (2007) Improved results are just a click away  Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, in vivo imaging, multicolor flow cytometry, nucleic acid quantitation, nucleosides
BioProbes article (2007) Putting the pH in phagocytosis  flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, microplate reader, multicolor flow cytometry, live-cell imaging, pH detection, phagocytosis, reagents, sample preparation
BioProbes article (2007) The use of Qdot nanocrystals in flow cytometry  flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, nanocrystals, protein labeling, Qdot, viability
BioProbes article (2007) Investigating apoptosis-induced loss of SYTO dye fluorescence  apoptosis, cell structure-mitochondria, enzyme activity detection, flow cytometer/flow cytometry, fluorescent dyes
BioProbes article (2007) New applications for Vybrant DyeCycle stains  Alexa Fluor, cell cycle, cell isolation, drug discovery, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, multicolor flow cytometry, stem cell research
BioProbes article (2007) Dead-cell discrimination in a different light  flow cytometer/flow cytometry, fluorescent dyes, viability
BioProbes article (2007) Multiparametric approaches for studying apoptosis  Alexa Fluor, antibodies, apoptosis, cell structure-mitochondria, cell structure-plasma membrane, enzyme activity detection, flow cytometer/flow cytometry, fluorescent dyes, viability
BioProbes article (2007) Listening in on cellular communication  cell signaling, fluorescent dyes, fluorescence microscopy/fluorescence imaging, ion indicators, microplate reader
BioProbes article (2008) Cell proliferation studies made simple  Alexa Fluor, cell proliferation, Click-iT, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nucleosides, sample stability
BioProbes article (2008) The picture of cell health  Alexa Fluor, cell health, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader, nucleic acid quantitation, nucleosides, reagents
BioProbes article (2008) A no-wash indicator for high-throughput calcium detection  BacMam technology, cell signaling, drug discovery, flow cytometer/flow cytometry, fluorescent proteins, high content analysis, ion flux/ion channels, ion indicators, microplate reader
BioProbes article (2008) Click chemistry: Making biodiscovery a snap  Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, in vivo imaging, nucleosides
BioProbes article (2008) Potassium flux assay meets BacMam technology  BacMam technology, cell signaling, fluorescent dyes, genes, fluorescence microscopy/fluorescence imaging, ion flux/ion channels, ion indicators, microplate reader, reagents
BioProbes article (2008) Nuclear receptor antibodies: A core technology  antibodies, cell signaling, ELISA, gene expression, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), ion flux/ion channels, western blotting
BioProbes article (2008) Flow cytometry: checking vital signs  Alexa Fluor, cell health, cell proliferation, CellTrace, Click-iT, enzyme activity detection, flow cytometer/flow cytometry, fluorescent dyes, nucleic acid quantitation, viability
BioProbes article (2008) Recent news in neurodegeneration  ELISA, apoptosis, cell health, fluorescent dyes, neuroscience, proteins, viability, western blotting
BioProbes article (2008) Evaluating cellular viability on human primary cells—alamarBlue reagent for cell viability and cell proliferation  cancer, cell proliferation, fluorescent dyes, microplate reader, viability
BioProbes article (2009) Modeling angiogenesis in vitro  Alexa Fluor, angiogenesis, cell culture, cell proliferation, Click-iT, fluorescent dyes, fluorescence microscopy/fluorescence imaging, membranes
BioProbes article (2009) Multiparametric approaches for predictive hepatotoxicity  cell structure-mitochondria, cell structure-plasma membrane, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress, viability
BioProbes article (2009) Beyond Classification—Click-iT EDU outperforms BRDU across all species  Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nucleic acid detection, nucleic acid quantitation, nucleosides, sample preparation
BioProbes article (2009) Innovative solutions for ion channel research  BacMam technology, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, ion flux/ion channels, ion indicators, microplate reader
BioProbes article (2009) Advances in neural stem cell culture  cell expansion, cell proliferation, Gibco, media, reagents, stem cell research, substrates
BioProbes article (2009) Live-cell imaging of cell cycle and cell division  BacMam technology, cell cycle, flow cytometer/flow cytometry, fluorescent proteins,  fluorescence microscopy/fluorescence imaging, high content analysis, live-cell imaging
BioProbes article (2009) A growing list of tools for the violet laser  Alexa Fluor, apoptosis, cell cycle, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, nanocrystals, Qdot, viability
BioProbes article (2009) Neurons in focus: Two-photon excitation imaging in live neuronal tissue  Alexa Fluor, fluorescent dyes, fluorescent proteins, fluorescence microscopy/fluorescence imaging, ion flux/ion channels, ion indicators, live-tissue imaging, nanocrystals, neuroscience, Qdot, two-photon microscopy
BioProbes article (2009) Isolate T cells directly from whole blood  cell isolation, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, immune system, magnetic beads
BioProbes article (2009) Traffic lights: Illuminating internalization pathways  drug discovery, flow cytometer/flow cytometry, fluorescent dyes, particles, pH detection, phagocytosis, reagents
BioProbes article (2009) Dead cell stains in flow cytometry: A comprehensive analysis  apoptosis, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, viability
BioProbes article (2009) The envelope, please: Best picture for biofilms  biofilm staining, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, microbiology, microplate reader, viability
BioProbes article (2009) High-content analysis of cytotoxicity  cell health, cell proliferation, Click-iT, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader, viability
BioProbes article (2009) A pHrodo dye-based method for monitoring the phagocytic internalization of apoptotic cells  apoptosis, fluorescent dyes, fluorescence microscopy/fluorescence imaging, pH detection, phagocytosis
BioProbes article (2009) Faster, easier calcium flux assays  cell signaling, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, ion flux/ion channels, microplate reader
BioProbes article (2009) Near-infrared dye for live cell cycle analysis  cell cycle, flow cytometer/flow cytometry, fluorescent dyes, live-cell imaging, nucleic acid detection
BioProbes article (2009) Cytotoxicity and cell proliferation studies—The CyQUANT Direct Cell Proliferation Assay  cell health, cell proliferation, fluorescent dyes, high content analysis, microplate reader, viability
BioProbes article (2009) Assess hERG channel blocks without radioactivity  cardiovascular system, cell signaling, drug discovery, fluorescent dyes, ion flux/ion channels, microplate reader
BioProbes article (2009) High-throughput chloride channel screening  BacMam technology, fluorescent proteins,  fluorescence microscopy/fluorescence imaging, high content analysis, ion flux/ion channels, ion indicators
BioProbes article (2009) TUNEL vision  apoptosis, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, sample preparation, sample stability
BioProbes article (2009) Automated cell counting at your fingertips  automated cell counter, cell counting, Countess, fluorescent dyes, viability
BioProbes article (2009) Fluorescence technologies for high-content imaging and analysis  Alexa Fluor, cell health, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress, protein detection, viability
BioProbes article (2010) Dead cell identification in flow cytometry flow cytometer/flow cytometry, viability
BioProbes article (2010) Putting autophagy into context autophagy, CellInsight fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress
BioProbes article (2010) Taking the pulse of cell proliferation cell proliferation, Click-iT 
BioProbes article (2010) Choosing the right dye for imaging intracellular calcium calcium flux, fluorescence microscopy/fluorescence imaging, high content analysis, ion channels, ion flux, ion indicators, reagents, sample preparation
BioProbes article (2010) Advanced cell proliferation analysis Attune/Attune NxT cell proliferation, cell tracking, flow cytometer/flow cytometry, fluorescence
BioProbes article (2010) Probes for visualizing secretory transport and autophagy autophagy, cell structure, cell structure-ER, cell structure-golgi, cell structure-mitochondria, CellLight, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2010) A closer look at mitochondrial dysfunction with MitoSOX Red Mitochondrial Superoxide Indicator cell structure-mitochondria, oxidative stress
BioProbes article (2010) Mitochondrial DNA synthesis exposed Click-iT, cell proliferation, cell structure-mitochondria
BioProbes article (2010) Cells under stress—Fluorescence-based tools for investigating autophagy apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2010) Dual pulse labeling of cell proliferation cell proliferation, Click-iT 
BioProbes article (2010) A ratiometric approach to apoptosis detection by flow cytometry apoptosis flow cytometer/flow cytometry, fluorescence
BioProbes article (2010) Alexa Fluor dyes shed light on a new actin assembly factor cell structure-actin, fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress
BioProbes article (2011) Tali Image-Based Cytometer—Benchtop GFP/RFP transfection and viability determination has arrived fluorescence microscopy/fluorescence imaging, fluorescent proteins, viability
BioProbes article (2011) Tools for measuring cell proliferation within a population or in a single cell cell cycle, cell differentiation, cell proliferation, microplate reader
BioProbes article (2011) Click-iT EdU Flow Cytometry Kits—Simplifying cell proliferation analysis cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence
BioProbes article (2011) Introducing the Tali Image-Based Cytometer—Three-parameter population analysis right from your bench apoptosis, fluorescent proteins, viability 
BioProbes article (2011) Accurate assessment of microbial viability by flow cytometry—LIVE/DEAD BacLight Bacterial Viability Kit meets the Attune Acoustic Focusing Cytometer Attune/Attune NxT, flow cytometer/flow cytometrymicrobiology, viability
BioProbes article (2011) Click-iT Labeling Kits detect biomolecules across multiple applications—Biological discovery is a click away cell proliferation, Click-iT EdU, fluorescence microscopy/fluorescence imaging
BioProbes article (2011) Fluorescence imaging of oxidative stress in live cells—CellROX Deep Red Reagent is a bright and photostable ROS sensor CellROX, fluorescence microscopy/fluorescence imaging, oxidative stress
BioProbes article (2011) Bringing cell death to light—CellEvent Caspase-3/7 Substrate and the multiplex analysis of apoptosis apoptosis, ArrayScan, CellEvent, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
BioProbes article (2011) Advances in high-content screening—Assays for multiparametric analysis of cell health ArrayScan, cell health, cell proliferation, cell structure-plasma membrane, Click-iT EdU, fluorescence microscopy/fluorescence imaging, high content analysis, viability
BioProbes article (2011) Endocytosis in disease and cell health cell health, cell structure, CellLight, endocytosis and phagocytosis, fluorescence microscopy/fluorescence imaging, high content analysis, pH detection
BioProbes article (2011) Shedding light on oxidative stress—CellROX Deep Red Reagent for ROS detection ArrayScan, cell health, CellROX, fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress
BioProbes article (2011) No-wash imaging of apoptosis—CellEvent Caspase-3/7 Green Detection Reagent for apoptosis apoptosis, ArrayScan, caspase substrates, CellEvent, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2012) Tracking internalization in live cells with pHrodo dyes endocytosis/phagocytosis, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, internalization, live-cell imaging, pHrodo
BioProbes article (2012) Streamline your oxidative stress measurements—CellROX Green and CellROX Orange reagents with simple workflows CellROX, fluorescence microscopy/fluorescence imaging, microplate reader, oxidative stress
BioProbes article (2012) Going viral—Fluorescent probes to image viruses and their host cell interactions cell proliferation cell tracking, Click-iT
BioProbes article (2012) Determine yeast concentration and viability at your benchtop—Yeast viability measurements with the Tali Image-Based Cytometer viability, yeast
BioProbes article (2012) Ready-to-use reagents shift the focus to results cell structure-nucleus, fluorescence microscopy/fluorescence imaging, high content analysis, sample preparation, viability
BioProbes article (2012) Unmasking cell replication—Reagents for analyzing cell cycle and replication in mammalian cells cell cycle, cell health cell proliferation, cell structure-nucleus, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging
BioProbes article (2013) ReadyProbes ready-to-use imaging reagents for brilliant results apoptosis, cell structure-all, fluorescence microscopy/fluorescence imaging, high content analysis, reagents, Imaging sample preparation, viability
BioProbes article (2013) Oxidative stress from a cell's point of view—From CellROX ROS sensors to Image-iT and Click-iT lipid peroxidation detection kits CellROX, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress
BioProbes article (2013) Checking vital signs: Don't let dead cells mislead you—Molecular Probes cell health assays for flow cytometry apoptosis, caspase substrate, cell cycle, cell health, cell proliferation, cell tracking, Click-iT, flow cytometer/flow cytometry,oxidative stress, viability
BioProbes article (2013) Autophagy made visible—Advanced fluorescent probes for imaging distinct processes in the autophagy pathway autophagy, cell structure, cell structure-lysosomes, CellLight, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2014) A sensitive assay for hyperploidy and cell death in the Drosophila brain using the Attune Flow Cytometer Attune/Attune NxT, cell cycle, cell death, cell health, flow cytometer/flow cytometry, fluorescent proteins, neuroscience
BioProbes article (2014) Image live cells through time and space—Optimizing conditions for time-lapse fluorescence microscopy cell structure, cell tracking, CellLight, EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, live-cell imaging, oxidative stress
BioProbes article (2014) Evaluate neural stem cells for neurodegenerative disease models—Neural stem cell–specific protein expression and mitochondrial assessment cell health, EVOS, FLoid, neuroscience, stem cell research
BioProbes article (2014) Multiplex your cell proliferation assay with GFP, RFP, and R-PE probes—Click-iT Plus EdU Proliferation Kits for imaging and flow cytometry cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, multicolor flow cytometry
BioProbes article (2014) Superior live-cell tracing, generation after generation—The CellTrace Far Red Cell Proliferation Kit cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescence
BioProbes article (2015) Applications for therapeutic monoclonal antibodies—Tools for studying antibody internalization and trafficking antibodies, antibody-drug conjugates, antibody labeling, drug research, endocytosis/phagocytosis, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC), internalization, pH detection
BioProbes article (2015) Tools to study mitochondrial morphology and function—Multiplex your mitochondrial data cell structure-mitochondria, fluorescence microscopy/fluorescence imaging, oxidative stress
BioProbes article (2015) High-content analysis applied to autophagy—Automating cell imaging with Thermo Scientific HCA systems ArrayScan, autophagy, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2015) Fluorescence-based viability assays for the Countess II FL Automated Cell Counter—Incorporate automation into your cell analysis workflows automated cell counter, cell counting, Countess, EVOS, fluorescence microscopy/fluorescence imaging, viability
BioProbes article (2015) Multiplexable Click-iT Plus TUNEL assays for in situ apoptosis detection—Visualize apoptosis in context apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2015) Formation of uniform and reproducible 3D cancer spheroids in high-throughput plates—Mimic life in three dimensions cancer, fluorescence microscopy/fluorescence imaging, hypoxia, oxidative stress, viability
BioProbes article (2015) Journal Club—Multiparameter detection of early apoptosis markers without compensation apoptosis, Attune/Attune NxT, cell structure-mitochondria, flow cytometer/flow cytometryimmunophenotyping
BioProbes article (2016) FluxOR II Green Potassium Ion Channel Assay—Next-generation detection of potassium ion flux high throughput screening, ion channels, ion flux, ion indicators
BioProbes article (2016) High-Speed Cell Counting With the Attune NxT Flow Cytometer—Accurately measure cell concentrations by flow cytometry Attune/Attune NxT, cell concentration, flow cytometer/flow cytometry, microbiology, viability
BioProbes article (2016) CyQUANT Direct and PrestoBlue Viability Assays work together—Multiplex assays for robust cell health analyses cell proliferation, CyQuant, microplate reader, PrestoBlue, viability
BioProbes article (2016) Click-iT EdU and Click-iT TUNEL Colorimetric Assays for immunohistochemistry—Proliferating and apoptotic cells revealed apoptosis, brightfield microscopy, cell proliferation, Click-iT, colorimetric,fixed-cell imaging, immunohistochemistry (IHC)
BioProbes article (2016) A guide to studying cell proliferation by generational tracing—Tracking cell division with flow cytometry CellTrace, cell cycle, cell proliferation, cell tracking, flow cytometer/flow cytometry
BioProbes article (2017) Appetite for destruction—Studying macroautophagy with Premo fluorescent protein–based sensors autophagy, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, immunocytochemistry (ICC), immunofluorescence (IF), live-cell imaging
BioProbes article (2017) Effectively monitor antibody internalization and trafficking—Improved labeling tools for antibody–drug conjugate screening and characterization antibodies, antibody-drug conjugates, antibody labeling, drug research, endocytosis/phagocytosis, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC), internalization, pH detection
BioProbes article (2017) Fueling discovery with high-content imaging and analysis fuel discovery—Thermo Scientific HCA platforms for automated, quantitative analyses high content analysis,  iPSCs, cell viability and function, CellInsight, drug research, neurite outgrowth, neuroscience, quantitative imaging
BioProbes article (2017) Optimize protein integrity and yields during workflows—Broad-spectrum protease and phosphatase inhibitor tablets cell cycle, CellTrace, flow cytometer/flow cytometry
BioProbes article (2017) An evaluation of dyes for tracking cell proliferation by flow and image cytometry cell proliferation, cell tracking, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging
BioProbes article (2017) Journal club—Measurement and characterization of apoptosis by flow cytometry flow cytometer/flow cytometry, apoptosis, fluorescent dyes
Molecular Probes Handbook MAXC Computer Program for calculating Free Ca2+ concentrations—Note 19.2 ion channels, ion flux, ion indicators
Molecular Probes Handbook Loading and calibration of intracellular ion indicators—Note 19.1 ion channels, ion flux, ion indicators
Molecular Probes Handbook Calcium regulation—Section 17.2 ion channels, ion flux, ion indicators
Molecular Probes Handbook Probes for ion channels and carriers—Section 16.3 ion channels, ion flux, ion indicators
Molecular Probes Handbook Probes useful at near-neutral pH—Section 20.2 BCECF, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, pH detection, pH indicators, SNARF
Molecular Probes Handbook Probes useful at acidic pH—Section 20.3 flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook pH indicator conjugates—Section 20.4 flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook Overview of pH indicators—Section 20.1 flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook Nucleic acid stains—Section 8.1 cell cycle, cell structure-nucleus, cell structure-plasma membrane, cell viability, DAPI, DNA binding dyes, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, membrane permeability, RNA binding dyes, SYTO, SYTOX
Molecular Probes Handbook Fluorescent Na+ and K+ indicators—Section 21.1 ion channels, ion flux, ion indicators
Molecular Probes Handbook Detecting chloride, phosphate, nitrite and other anions—Section 21.2 ion channels, ion flux, ion indicators
Molecular Probes Handbook Introduction to Ca2+ measurements with fluorescent indicators—Section 19.1 ion channels, ion flux, ion indicators
Molecular Probes Handbook Fluorescent Mg2+ indicators—Section 19.6 ion channels, ion flux, ion indicators
Molecular Probes Handbook Fluorescent indicators for Zn2+ and other metal ions—Section 19.7 ion channels, ion flux, ion indicators
Molecular Probes Handbook Fluorescent Ca2+ indicators excited with visible light—Section 19.3 ion channels, ion flux, ion indicators
Molecular Probes Handbook Fluorescent Ca2+ indicators excited with UV light—Section 19.2 ion channels, ion flux, ion indicators
Molecular Probes Handbook Fluorescent Ca2+ indicator conjugates—Section 19.4 ion channels, ion flux, ion indicators
Molecular Probes Handbook Chelators, calibration buffers, ionophores and cell-loading reagents—Section 19.8 ion channels, ion flux, ion indicators
Molecular Probes Handbook Protein-based Ca2+ indicators—Section 19.5 calcium flux, fluorescent dyes, ion indicators
Molecular Probes Handbook Viability and cytotoxicity assay reagents—Section 15.2 cell health, cell viability and function, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes Handbook Viability and cytotoxicity assay kits for diverse cell types—Section 15.3 cell health, cell viability and function, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes Handbook Overview of probes for cell viability, cell proliferation and live-cell function—Section 15.1 cell health, cell death, cell proliferation, cell viability and function
Molecular Probes Handbook Assays for apoptosis and autophagy—Section 15.5 apoptosis, autophagy, fluorescent dyes, fluorescence microscopy/fluorescence imaging, flow cytometer/flow cytometry
Protocol Vybrant MTT Cell Proliferation Assay Kit cell viability, microplate
Protocol Viability staining protocol for flow cytometry cell viability, flow cytometry
Protocol SYTOX Dead Cell Stains cell viability, flow cytometry
Protocol Red blood cell lysis protocols using eBioscience lysis buffers cell proliferation, flow cytometry
Protocol ReadyProbes Cell Viability Imaging Kit, Blue/Red cell viability, imaging
Protocol ReadyProbes Cell Viability Imaging Kit, Blue/Green cell viability, imaging
Protocol PrestoBlue Cell Viability Reagent for microplates cell viability, microplate
Protocol PrestoBlue and CyQUANT Direct Confirmation Assay cell viability, microplate
Protocol Pharmacological induction of apoptosis with camptothecin cell vitality, flow cytometry, high content analysis, imaging, microplate
Protocol NucRed Dead 647 ReadyProbes Reagent for viability cell viability, imaging
Protocol NucGreen Dead 488 ReadyProbes Reagent for viability cell viability, imaging
Protocol LIVE/DEAD Yeast Viability Kit cell viability, imaging
Protocol LIVE/DEAD Violet Viability/Vitality Kit cell viability and cell vitality, flow cytometry
Protocol LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells cell viability, imaging
Protocol LIVE/DEAD Sperm Viability Kit for flow cytometry cell viability, flow cytometry
Protocol LIVE/DEAD Sperm Viability Kit for imaging cell viability, imaging
Protocol LIVE/DEAD Fixable Dead Cell Stains cell viability, flow cytometry
Protocol LIVE/DEAD Cell Imaging Kit (488/570) cell viability, imaging
Protocol LIVE/DEAD BacLight Bacterial Viability Kit cell viability, imaging
Protocol LIVE BacLight Bacterial Gram Stain Kit cell viability, imaging
Protocol Image-iT DEAD Green Kit cell viability, imaging
Protocol HCS Mitochondrial Health Kit cell viability, high content analysis
Protocol HCS LIVE/DEAD Green Kit using Hoechst 33342 cell viability, high content analysis
Protocol HCS LIVE/DEAD Green Kit using HCS NuclearMask Deep Red cell viability, high content analysis
Protocol Staining cells with eFluor proliferation dyes for flow cytometry cell proliferation, flow cytometry
Protocol CyQUANT Direct Microplate Reagent for cell viability cell viability, microplate
Protocol Click-iT Plus EdU imaging kits cell proliferation, imaging
Protocol Click-iT EdU imaging kits cell proliferation, imaging
Protocol Click-iT EdU HCS assays cell proliferation, imaging
Protocol CellTrace Violet Cell Proliferation Kit cell proliferation, flow cytometry
Protocol CellTrace Far Red Cell Proliferation Kit cell proliferation, flow cytometry
Protocol CellTrace CFSE Cell Proliferation Kit cell proliferation, flow cytometry
Protocol Cell preparation for flow cytometry protocols cell proliferation, flow cytometry
Protocol BrdU staining protocol for flow cytometry cell proliferation, flow cytometry
Protocol BrdU labeling and detection cell proliferation, imaging
Protocol Annexin V staining protocol for flow cytometry cell vitality, flow cytometry
Scientific poster (2006) LipidTOX dyes for adipocyte staining in routine imaging applications  cell differentiation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, stem cell research, viability
Scientific poster (2006) Cell cycle analysis using microplate cytometry: A comparison of laser and dye combinations  Acumen Explorer, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Scientific poster (2006) New fluorescent reagents for the imaging-based assays for the analysis of drug-induced perturbations of cellular lipid metabolism  ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, microplate reader, viability
Scientific poster (2006) Utility of 405 nm-excitable dyes in high content screening using an Acumen Explorer Microplate Cytometer  Acumen Explorer, apoptosis, cell cycle, cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, violet laser-excited reagents
Scientific poster (2006) Novel violet-excited reagents for detection of viability and vitality   Alexa Fluor, cell health, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, viability, violet laser-excited reagents
Scientific poster (2007) Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry  antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2007) A new approach for the detection of intracellular glutathione by fluorescence microscopy and flow cytometry  ArrayScan, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, live-cell imaging, microplate reader, viability, violet laser-excited reagents
Scientific poster (2007) Click chemistry-based detection of S-phase adherent cells using automated microscopy and image analysis  Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Scientific poster (2007) Click catalyzed nucleic acid labeling as a novel replacement for BrdU antibody-based cell proliferation assay  Alexa Fluor, antibodies, antibody labeling, cell cycle, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling
Scientific poster (2008) Living molecular probes for the next generation  BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, ion flux/ion channels, ion indicators, live-cell imaging
Scientific poster (2008) Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents  Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Scientific poster (2008) Cell-based potassium ion channel screening with the FluxOR Assay  BacMam technology, cell counting, Countess, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2008) Novel Click-IT TUNEL Assay for detection of cell death  Alexa Fluor, antibodies, apoptosis, Click-iT, cytotoxicity, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, immunocytochemistry (ICC), viability
Scientific poster (2008) Effects of the thymidine analogues EdU and BrdU on cell viability and cycle progression  Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, viability
Scientific poster (2008) Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry  Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, nucleic acid quantitation
Scientific poster (2008) Improving downstream results with automated cell counting  automated cell counter, BacMam technology, cell counting, cell expansion, cell isolation, Countess, fluorescent dyes, hemocytometer, ion flux/ion channels, microplate reader
Scientific poster (2008) FluxOR, a universal potassium channel assay: Screening BacMam-delivered and stably expressed targets  BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2008) Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method  Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling
Scientific poster (2009) Cell-based assays for predictive hepatotoxicity measurements using high content imaging  Alexa Fluor, ArrayScan, cell structure-plasma membrane, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, nucleic acid quantitation, viability
Scientific poster (2009) A non-radioactive method for detecting nascent protein synthesis by high-content imaging utilizing click chemistry  Alexa Fluor, apoptosis, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, protein detection
Scientific poster (2009) Monitoring mitotic cells and DNA content by automated imaging and analysis  Alexa Fluor, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability
Scientific poster (2009) Dual pulse labeling of S-phase population using click chemistry to measure changes in cell proliferation  Alexa Fluor, cancer, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes
Scientific poster (2009) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry  Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents
Scientific poster (2009) Dual pulse labeling using a new thymidine analog 5-ethynyl-2'-deoxyuridine (EdU) to detect alterations in S-phase progression by fluorescence microscopy and flow cytometry  Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2009) High content imaging of endocytosis & phagocytosis using pHrodo conjugates  Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability
Scientific poster (2009) Click chemistry-based detection of nascent RNA synthesis using high content imaging and fluorescence microscopy  Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, viability
Scientific poster (2009) A simple, fast and quantitative single-step dead-cell indicator for flow cytometry  apoptosis, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability
Scientific poster (2009) High content analysis of cytotoxicity by laser scanning fluorescence microplate cytometry  Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, viability
Scientific poster (2009) Accuracy and precision comparison of the hemocytometer and automated cell counting methods  automated cell counter, cell counting, Countess, hemocytometer, particles, viability
Scientific poster (2009) Dead cell stains in flow cytometry: A comprehensive analysis  apoptosis, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, viability
Scientific poster (2009) High content imaging and analysis of mitotoxicity and cytotoxicity in fixed cells  cell structure-mitochondria, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, viability
Scientific poster (2009) Quantitative analysis of genotoxicity and cytotoxicity to DNA damaging agents using high-content imaging  Alexa Fluor, antibodies, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability
Scientific poster (2009) Cell-based potassium ion channel screening with BacMam using the FluxOR Assay  BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2009) Click chemistry-based assays for fluorescence microscopy and high content imaging  Alexa Fluor, apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid detection, protein detection, viability
Scientific poster (2010) Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry  apoptosis, autophagy, caspase substrates, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress
Scientific poster (2010) Acoustic focusing cytometry: Sensitivity and throughput  apoptosis, Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, phagocytosis
Scientific poster (2010) High content imaging of autophagy  antibodies, ArrayScan, autophagy, BacMam technology, cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader
Scientific poster (2010) Analysis of proliferation dynamics in primary and immortalized human cells  Alexa Fluor, cancer, cell proliferation, CellTrace, circulatory system, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescent proteins, nucleic acid quantitation
Scientific poster (2010) Oxidative stress-induced protein modification: Application of clickable linoleic acid analogs  Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, microplate reader, oxidative stress, protein detection, protein enrichment
Scientific poster (2010) Antibody- and fluorescent protein-based approaches to measuring autophagy in mammalian cells by fluorescence microscopy  Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging
Scientific poster (2010) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging  Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents
Scientific poster (2010) Novel tools to enable high resolution gene expression by investigating the nascent transcriptome  Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, magnetic beads, nucleic acid labeling, PCR/qPCR, viability
Scientific poster (2010) Autophagosomal accumulation perturbs Golgi structure without affecting other organelles: Implications for autophagosome biogenesis  Alexa Fluor, antibodies, autophagy, BacMam technology, cell structure-golgi, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging
Scientific poster (2010) A new generation of cellular imaging probes for neurosciences research  autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators
Scientific poster (2010) Simultaneous analysis of cell death mechanisms and oxidative stress using live cell fluorescence microscopy  apoptosis, ArrayScan, BacMam technology, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader, oxidative stress
Scientific poster (2010) A comparison of three techniques to induce efficient ex vivo T cell expansion  Alexa Fluor, antibodies, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, immune system, magnetic beads, multicolor flow cytometry, viability
Scientific poster (2010) New fluorescent probes for live-cell imaging  autophagy, cell cycle, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, pH detection, phagocytosis
Scientific poster (2010) Functional characterization of a novel fluorescent dye for proliferation analysis  Alexa Fluor, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, violet laser-excited reagents
Scientific poster (2010) Non-cytotoxic near-IR DNA stain for cell cycle analysis in living cells  cell cycle, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, live-cell imaging, nucleic acid labeling, viability
Scientific poster (2010) BacMam gene expression for functional and imaging applications  BacMam technology, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels
Scientific poster (2010) Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry  Alexa Fluor, cell cycle, cell proliferation, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2011) Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health  ArrayScan, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress, viability
Scientific poster (2011) Simultaneous analysis of cell death mechanisms and oxidative stress using high content imaging  Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2011) Primary human cells from Life Technologies: Offerings, scale and applications in HCS and HTS formats  Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, cell signaling, Click-iT, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Scientific poster (2011) Validation of high content imaging and analysis assays for cell health and cytotoxicity  antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, cell health, cell proliferation, CellLight, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, pH detection
Scientific poster (2011) The next step in the analysis of lymphocyte proliferation  Alexa Fluor, antibodies, Attune/Attune NxT, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, live-cell imaging, violet laser-excited reagents
Scientific poster (2011) The next generation of cell-based imaging assays for apoptosis, autophagy and oxidative stress from Molecular Probes  Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2011) Application of acoustic cytometry in microbiology  Attune/Attune NxT, cell concentration, cell health, flow cytometer/flow cytometry, fluorescent dyes, microbiology, viability
Scientific poster (2012) Rapid, in vitro T lymphocyte cell tracing by acoustic focusing cytometry  Attune/Attune NxT, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, magnetic beads, stem cell research
Scientific poster (2012) High sensitivity applications for the Applied Biosystems Attune  Attune/Attune NxT, caspase substrates, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, viability
Scientific poster (2012) Cell-based analysis of oxidative stress, lipid peroxidation and lipid peroxidation-derived protein modifications using fluorescence microscopy  flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis, lipid peroxidation, microplate reader, oxidative stress, viability
Scientific poster (2013) Pacific Green dye: A new fluorophore for violet laser excitation  Alexa Fluor, antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, viability, violet laser-excited reagents
Scientific poster (2013) Monitor caspase 3/7 activity without cell fixation: A novel apoptosis reagent from Molecular Probes  Alexa Fluor, apoptosis, Attune/Attune NxT, caspase substrates, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry
Scientific poster (2013) Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health  apoptosis, ArrayScan, caspase substrates, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress
Scientific poster (2013) Reactive oxygen probes—A broad range of colors with easier labeling: Novel CellROX reagents from Molecular Probes  cells, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, oxidative stress, viability
Scientific poster (2013) Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping  Alexa Fluor, Attune/Attune NxT, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping
Scientific poster (2013) Reactive oxygen probes—A broad range of colors with easier labeling and compatibility with fixation: Novel CellROX reagents from Molecular Probes  cells, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, oxidative stress
Scientific poster (2013) Cells coming to life: Tools for visualizing immune response  Attune/Attune NxT, endocytosis/phagocytosis, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunophenotyping, live-cell imaging, pH detection
Scientific poster (2013) Illuminating endocytosis with targeted pH-sensitive fluorescent compounds  Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability
Scientific poster (2014) Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detection  Attune/Attune NxT, cell proliferation, Click-iT, flow cytometer/flow cytometry, multicolor flow cytometry
Scientific poster (2014) New fluorescent probes and sensors for visualizing endocytosis, lysosomal dynamics and autophagy  autophagy, cell structure-lysosomes, endocytosis/phagocytosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader
Scientific poster (2014) Click-iT EdU Plus for flow cytometry: enhanced compatibility for multiplexing  Attune/Attune NxT, cell proliferation, Click-iT, flow cytometer/flow cytometry, multicolor flow cytometry
Scientific poster (2015) A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer  Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, immunophenotyping, no lyse, phagocytosis, whole blood
Scientific poster (2015) Colorimetric dual labeled EdU/BrdU technology demonstrates contextual information and dynamics of proliferation in tissue  apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Scientific poster (2015) Visualizing the life and death of cells: novel probes and platforms  apoptosis, ArrayScan, autophagy, cell death, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis
Scientific poster (2016) High content screening in MCF7 and MDA-MB231 cells show differential responses depending on oxygen levels and mechanistic readout for viability  cell health, cell viability and function, drug discovery, high content analysis, high throughput screening
Scientific poster (2016) Cell mediated cytotoxicity in "untouched" whole blood  Attune/Attune NxT, cell concentration, cell cycle, flow cytometer/flow cytometry, immunophenotyping, no lyse, whole blood
Scientific poster (2016) A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry  Attune/Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging
Scientific poster (2016) Click colorimetric EdU proliferation and TUNEL: Click chemistry for brightfield microscopy  apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Video CellEvent Caspase 3/7 reagent and apoptosis detection 
HeLa cells were loaded with 50 nM TMRM (red) followed by 5 μM CellEvent Caspase 3/7 Substrate (green). Cells were then treated with 0.5 μM staurosporine to induce apoptosis. TMRM detects the mitochondrial membrane potential and the signal disappears as the apoptosis onsets. CellEvent detects apoptosis, with green signal.
apoptosis, cell health, CellEvent, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Viability determination of HeLa cells using ReadyProbes Cell Viability Imaging Kit (Blue/Green) 
HeLa cells were loaded with NucBlue Live and NucGreen Dead (using 2 drops per ml) in complete media for 15 minutes at 37C. Stauroporine was then added to a final concentration of 1 µM and images were acquired every 30 min. for 18 hours using EVOS Auto Imaging System. All cells are stained with NucBlue Live, shown with blue nuclei. Over time an increase in the number of dead cells is observed as indicated by the appearance of green nuclei (NucGreen Dead).
cell structure-nucleus, cell viability, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video LIVE DEAD Cell Imaging kit on the EVOS Auto Imaging System 
Time course of cell death visualized using the LIVE/DEAD Cell Imaging kit (R37601). The LIVE/DEAD Cell Imaging kit is based on a cell-permeable dye (calcein, AM) that stains live, viable cells bright green and a cell-impermeable red marker that only stains dead and dying cells, which are characterized by compromised cell membranes. Labeled U-2 OS cells were treated 1 μM staurosporine and fluorescence images in the FITC and TexasRed channels were acquired every 5 minutes over 14 h on the EVOS Auto Imaging System using a 20x objective.
cell viability, fluorescence microscopy/fluorescence imaging, ion channels, ion flux, ion indicators, live-cell imaging
Video Compilation of live cell imaging videos using Invitrogen fluorescent reagents 
This video demonstrates novel product brands from Invitrogen for live cell imaging include CellLight targeted fluorescent proteins, CellROX reagents for oxidative stress, CellEvent caspase 3/7 detection reagent, and many more fluorescent dyes and probes.
cell structure-mitochondria, CellEvent, CellLight, CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress
Video Click-iT Plus TUNEL apoptosis assays 
The Click-iT Plus TUNEL assay detects apoptotic cells in tissue and cultured cell samples through the use of a small, highly specific labeling moiety and a bright fluorescent dye. After incorporation of the labeling moiety into DNA fragments, detection is achieved through a catalyzed "click" reaction using conditions mild enough to preserve the fluorescent signal from GFP or RFP.
apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging
Video Z Stack image of HeLa cells labeled with CellLights reagents 
A series of images were captured on the EVOS FL Auto Cell Imaging System. Creating a Z-stack from these images allowed the observation of cellular cytoskeletal changes, which can be indicative of the loss of cell health. Methods HeLa cells grown in MatTek 6-well glass bottom culture plates were transduced with CellLights Tubulin-GFP and CellLight Mitochondria-RFP overnight at 37oC. The following day, NucBlue Live reagent (2 drops/mL) was added to the cultures. Cells were then imaged on an EVOS FL Auto Cell Imaging System with 100x oil immersion objective using the Z-stack function. The step size was set using the Nyquist formula and performed at 0.366 μm.
cell health, cell structure-all, CellLight, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging
Video Detection of oxidative stress with CellROX Green in U2-OS cells 
U2-OS cells were plated at 200,000 cells per dish on a 35 mm glass bottom dish (MatTek) and cultured overnight. The cells were rinsed once and loaded with 5 µM CellROX Green Reagent (Cat. No. C10444) and 100 nM TMRM in Live Cell Imaging Solution (LCIS, Cat. No. A14291DJ) for 15 minutes at 37 degrees C. After loading, cells were imaged live with no washing every 20 seconds for seventy minutes on the DeltaVision Core inverted microscope at 37 degrees C. Menadione was added to a final concentration of 100 uM after ten minutes of baseline acquisition. The time lapse data shows the loss of signal from TMRM in the red channel as mitochondrial function decreases, concomitant with the onset of a nuclear signal in the green channel as CellRox Green is oxidized and the reagent migrates to the nucleus to generate a fluorogenic response as the active form of the dye binds to DNA.
CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress
Video Time-lapse of cell migration and apoptosis during angiogenesis  
Angiogenesis pseudo-tube formation and apoptosis shown with time-lapse imaging. The time-lapse images were captured and assembled using an EVOS FL Auto Imaging System equipped with the EVOS Onstage Incubator.
angiogenesis, apoptosis, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator
Video Click-iT Plus EdU Proliferation Assay 
Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies.
cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging
Webinar Basic techniques in autophagy research 
This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy. Topics to be covered include: Tips and tricks for selecting the right tools and achieving the best results Fluorescent proteins and antibodies used to analyze both live and fixed cells Analysis steps using a variety of multiplexing options, with quantitative methods for image analysis or fluorescence intensity measurement
autophagy, cell health, fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, immunofluorescence (IF)
Webinar Basics of multicolorflow cytometry panel design 
With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters multicolor flow cytometry has become more popular and more accessible than ever. This webinar presented by Dr. Holden T. Maecker at Stanford University will discuss the caveats of good panel design, including: Rules for designing panels Examples and practical application of these rules Controls and standardization Relevance of panel design to new mass cytometry platforms
antibodies, compensation, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry, viability
Webinar The meaning of life at the cellular level: detecting apoptosis with fluorescence 
In this webinar, Thermo Fisher Scientific scientists will demonstrate several solutions using fluorescent probes to study key components of the apoptotic machinery. The presentation includes: An overview of the features of apoptosis Key parameters that can be measured to assay apoptosis A comprehensive guide to available labeling and detection technologies for apoptosis research Tips and tricks to best implement those technologies
apoptosis, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent proteins
Webinar DNA content cell cycle analysis using flow cytometry 
Find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis. In this webinar we'll discuss: An overview of the methods and materials for using flow cytometry to determine cell cycle by measuring DNA content Selection of DNA dyes for live cell and fixed cell analysis Tips and tricks for consistent results
cell counting, cell cycle, cell proliferation, flow cytometer/flow cytometry
Webinar An introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis 
In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring Membrane structure, Mitochondrial function, Metabolism, Caspase activity, Membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.
apoptosis, cell cycle, cell proliferation, flow cytometer/flow cytometry, multicolor flow cytometry, viability
Webinar The meaning of life at the cellular level: Probing viability with fluorescence 
This webinar will provide an overview of features of healthy and unhealthy cells as well as describing key parameters that can be measured to assay cell viability. The webinar also offers a comprehensive guide to available labeling and detection technologies for cell health research as well as tips and tricks on how to best use them.
cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, viability
Webinar Flow cytometry in microbiological research 
In recent years the application of flow cytometry in microbiological research has expanded from detection and quantification of organisms to more complex studies including analysis of host-microbe interactions and detailed spatial and temporal analysis of microbial metabolism in different environments. During this webinar we will discuss how the multi-parametric nature of flow cytometry can be applied to microbiology and the advantages of using this application over traditional microbiological methods.
flow cytometer/flow cytometry, microbiology, multicolor flow cytometry, viability
Webinar An introduction to flow cytometric analysis, Part 1: Cell proliferation analysis 
In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis.
apoptosis, cell cycle, cell proliferation, flow cytometer/flow cytometry, multicolor flow cytometry, viability
Webinar From the hood to the microscope; Revolutionizing cell-based imaging 
Join us for an interactive, educational webinar that showcases some of the latest advancements in cell-based research and imaging. Learn as we explore how to obtain superior results through the careful selection of reagents and the optimization of your imaging workflow— from growing cells on suitable surfaces through to image capture. Particular attention will be given to growing and monitoring cells for imaging, fluorescent labeling of live cells, critical considerations for time-lapse imaging and optimizing live cell imaging. Topics include: Choose a suitable imaging culture vessel for studying cell growth and viability using bright-field microscopy Label cells to maximize signal-to-noise for fluorescence imaging Prepare culture conditions and capture images without losing temporal data for time-lapse imaging Achieve hypoxic culture conditions by modifying gas conditions or employing cell spheroid cultures
cell health, EVOS, FLoid, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging, onstage incubator