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Invitrogen
CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), PE, eBioscience™
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Product Details
12-9400-41
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Non-human primate,
Sheep,
Pig,
Rabbit,
Rat
Published species
Dog,
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
ME20.4
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody ME20.4 recognizes CD271, a 75 kDa, type I transmembrane protein that belongs to the low-affinity neurotrophin receptor and tumor necrosis factor receptor superfamily. CD271 is expressed by neurons, Schwann cells, melanocytes, mesenchymal stem/stromal cells, neural crest stem cells, astrocytes, oligodendrocytes, and a subset of lymphoid cells including follicular dendritic cells. CD271 binds to the ligands NGF, BDNF, NTF3, and NTF4. CD271 contains a death domain which induces apoptosis and regulates cell growth and proliferation through interactions with TRAF2, TRAF4, TRAF6, PTPN13, and RANBP9.
The ME20.4 monoclonal antibody recognizes human, non-human primate, mouse, rat, sheep, rabbit, cat, dog, and pig CD271.
Applications Reported: This ME20.4 antibody has been reported for use in flow cytometric analysis, western blotting, and microscopy.
Applications Tested: This ME20.4 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated HEK-293T cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Neurotrophic factors control the survival, differentiation and maintenance of neurons in the peripheral and central nervous systems, and of other neural crest-derived cell types. Developing sympathetic neurons are absolutely dependent upon nerve growth factor (NGF) during the period of target competition in vivo. Antibodies reacting specifically with NGFR p75 are useful tools in the detection and characterization of NGFR p75, to enhance our understanding of a wide range of phenomena in the development, plasticity and repair of the nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD271; CD271 antigen; Gp80-LNGFR; low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; Low-affinity nerve growth factor receptor p75NGFR; Low-affinity nerve growth factor receptor p75NGR; nerve growth factor receptor (TNFR superfamily, member 16); nerve growth factor receptor, fast; NGF receptor; p-75; p75 ICD; p75 neurotrophin receptor; p75 ntr; TNFR superfamily, member 16; TNR16; Tumor necrosis factor receptor superfamily member 16; unnamed protein product
Gene Aliases: CD271; Gp80-LNGFR; LNGFR; NGFR; NGR; p75; p75(NTR); p75NGFR; p75NTR; RNNGFRR; TNFRSF16
UniProt ID: (Human) P08138, (Rat) P07174, (Mouse) Q9Z0W1
Entrez Gene ID: (Pig) 100525607, (Human) 4804, (Sheep) 101119064, (Dog) 491071, (Rabbit) 100349095, (Rat) 24596, (Mouse) 18053
beta-amyloid binding
transmembrane signaling receptor activity
death receptor activity
protein binding
calmodulin binding
coreceptor activity
small GTPase binding
ubiquitin protein ligase binding
signaling receptor activity
neurotrophin binding
nerve growth factor binding
neurotrophin TRKA receptor binding
Rab GTPase binding
preprotein binding
transmembrane signal receptor
cellular glucose homeostasis
hair follicle development
intracellular protein transport
apoptotic process
signal transduction
Rho protein signal transduction
nervous system development
axon guidance
central nervous system development
circadian rhythm
fibroblast growth factor receptor signaling pathway
regulation of gene expression
detection of temperature stimulus
nerve development
cell differentiation
negative regulation of cell migration
hair follicle morphogenesis
circadian regulation of gene expression
dorsal aorta development
negative regulation of fibroblast growth factor receptor signaling pathway
regulation of cell proliferation
odontogenesis of dentin-containing tooth
positive regulation of odontogenesis of dentin-containing tooth
glucose homeostasis
positive regulation of apoptotic process
skin development
fibroblast proliferation
positive regulation of fibroblast proliferation
rhythmic process
neuron apoptotic process
negative regulation of hair follicle development
extrinsic apoptotic signaling pathway
presynaptic modulation of chemical synaptic transmission
positive regulation of protein localization to nucleus
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis
cellular response to beta-amyloid
positive regulation of endothelial cell apoptotic process
positive regulation of apoptotic signaling pathway
response to wounding
positive regulation of cell death
negative regulation of neuron projection development
programmed cell death
sensory perception of pain
positive regulation of myelination
positive regulation of synaptic transmission, cholinergic
response to lipopolysaccharide
cellular response to oxidative stress
positive regulation of Rho protein signal transduction
skeletal muscle cell differentiation
positive regulation of MAPK cascade
negative regulation of neuron apoptotic process
positive regulation of protein kinase B signaling
negative regulation of mitochondrial depolarization
positive regulation of synaptic transmission, glutamatergic
negative regulation of cell death
positive regulation of neuron death
regulation of reactive oxygen species metabolic process
regulation of glucose import in response to insulin stimulus
multicellular organism development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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