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CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), Biotin, eBioscience™
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-Flow-20170216130641.jpg?time=20240507 "CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 57
CD49f (Integrin alpha 6) Antibody (13-0495-82) in Flow
Staining of normal human peripheral blood cells with 0.25 µg of Rat IgG2a kappa Isotype Control Biotin (Product # 13-4321-82) (open histogram) or 0.125 µg of Anti-Human/Mouse CD49f (Integrin alpha 6) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
-Flow-20170216130641.jpg?time=20240507)
-Flow-20170216130641.jpg?time=20240507?time=20240507 "CD49f (Integrin alpha 6) Antibody (13-0495-82) in Flow")
Product Details
13-0495-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioGoH3 (GoH3)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex; CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The eBioGoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.
Applications Reported: This eBioGoH3 (GoH3) antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.
Applications Tested: This eBioGoH3 (GoH3) antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CDf (Integrin alpha-6, ITGA6) is the integrin alpha chain alpha 6 protein. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signaling. Two transcript variants encoding different isoforms have been found for CDf. Functionally, integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals that affect cellular shape, mobility, and progression through the cell cycle in response to the extracellular matrix. Integrin receptors are composed of alpha and beta subunits, and form structural and functional linkages between the ECM (extra-cellular matrix) and intracellular cytoskeletal linker proteins. Signaling mediated by Intergrin/ECM interactions are also integrated with cellular responses to growth factor signaling to regulate cellular proliferation, cytoskeletal reorganization and other responses necessary for cellular survival. CDf is predominantly expressed by epithelia, and plays a critical structural role in the hemidesmosome. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD49 antigen-like family member F; CD49f; DKFZp686J01244; extracellular matrix receptor subunit; FLJ18737; Integrin alpha-6; integrin alpha6B; integrin, alpha 6; unnamed protein product; very late activation protein 6; VLA-6
Gene Aliases: 5033401O05Rik; AI115430; CD49f; ITGA6; ITGA6A; ITGA6B; JEB6; VLA-6
UniProt ID: (Human) P23229, (Mouse) Q61739
Entrez Gene ID: (Human) 3655, (Mouse) 16403
cell-substrate junction assembly
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
ectodermal cell differentiation
positive regulation of neuron projection development
positive regulation of cell migration
cell-substrate adhesion
nail development
positive regulation of apoptotic process
positive regulation of GTPase activity
skin morphogenesis
positive regulation of transcription from RNA polymerase II promoter
leukocyte migration
cell-cell adhesion
negative regulation of extrinsic apoptotic signaling pathway
positive regulation of cell-substrate adhesion
single organismal cell-cell adhesion
positive regulation of cell-cell adhesion
cellular response to extracellular stimulus
cell adhesion mediated by integrin
positive regulation of phosphorylation
odontogenesis of dentin-containing tooth
skin development
filopodium assembly
digestive tract development
brown fat cell differentiation
renal system development
amelogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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