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Invitrogen
AID Monoclonal Antibody (mAID-2), Biotin, eBioscience™
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FIGURE: 1 / 14
AID Antibody (13-5959-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using low pH antigen retrieval and stained with 20 µg/mL Rat IgG2a K Isotype Control Biotin (left) or 20 µg/mL Anti-Human/Mouse Activation-Induced Cytidine Deaminase (AID) Biotin (right) followed by Avidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin.
Product Details
13-5959-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
mAID-2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal mAID-2 recognizes human and mouse AID (gene AICDA) also known as Activation-Induced Cytidine Deaminase. AID is a 24 kDa RNA-editing enzyme that converts cytosine into uracil. It plays a critical role during B cell development, specifically in somatic hypermutation (SHM) and immunoglobulin class switch recombination (CSR). Changes in levels of AID expression typically present with disease. For example, mutations in AID are found in Hyper-IgM Syndrome (type 2), a disease form without opportunistic infections, while overexpression correlates with poor prognosis in chronic lymphocytic leukemia/small lymphocytic lymphoma.
AID is expressed mainly by activated mature B cells such as mature germinal centre (GC) B cells but also in intermediate GC cells (defined as IgD+CD38-CD23-CD71+). In addition to hematopoietic cells, AID is expressed in primary human hepatocellular carcinomas, helicobacter infected gastric epithelial cells, oocytes and embryonic stem cells although it role in these cells is not well understood. Because AID contains an NLS (nuclear localization signal), expression can be found both in the nucleus as well as in the cytoplasm.
Applications Reported: This mAID-2 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.
Applications Tested: This mAID-2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal perfomance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: activation induced deaminase; Activation-induced cytidine deaminase; AID; AID isoform AIDins(IVS3); Cytidine aminohydrolase; epididymis secretory protein Li 284; integrated into Burkitt's lymphoma cell line Ramos; Single-stranded DNA cytosine deaminase
Gene Aliases: AICDA; AID; ARP2; CDA2; HEL-S-284; HIGM2
UniProt ID: (Human) Q9GZX7, (Mouse) Q9WVE0
Entrez Gene ID: (Human) 57379, (Mouse) 11628
mRNA processing
regulation of macromolecule biosynthetic process
somatic diversification of immunoglobulins
somatic hypermutation of immunoglobulin genes
cytidine to uridine editing
B cell differentiation
regulation of nuclear cell cycle DNA replication
defense response to bacterium
hypomethylation of CpG island
isotype switching
negative regulation of single stranded viral RNA replication via double stranded DNA intermediate
defense response to virus
DNA cytosine deamination
cellular response to lipopolysaccharide
cytidine deamination
DNA demethylation
negative regulation of methylation-dependent chromatin silencing
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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