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Invitrogen
MULT1 (NKG2D Ligand) Monoclonal Antibody (5D10), eBioscience™
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FIGURE: 1 / 1
MULT1 (NKG2D Ligand) Antibody (14-5863-82) in Flow
Staining of the A20 cell line with 1 µg of Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (red histogram) or 1 µg of Anti-Mouse/Rat MULT1 (NKG2D Ligand) Purified (black histogram) followed by Anti-Armenian Hamster IgG PE (Product # 12-4112-83).Total viable cells were used for analysis.
Product Details
14-5863-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
5D10
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 5D10 monoclonal antibody reacts with the murine MULT-1 (Murine ULBP-Like Transcript 1). MULT-1 is a type I transmembrane protein with two Ig-like domain and is one of the known murine NKG2-D ligands that include RAE1 molecules (alpha, beta, epsilon, gamma and delta) and H60. MULT-1 is a high affinity ligand for NKG2D.Expression of NKG2-D ligands is low or absent on normal adult tissues. However, stressed or transformed cells express NKG2-D ligand and can activate NK cell tumoricidal activity. Until now, the expression of NKG2-D ligands has been mainly studied with NKG2-D tetramers recognizing all NKG2-D ligands.
Applications Reported: This 5D10 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.
Applications Tested: This 5D10 antibody has been tested by flow cytometric analysis of the A20 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ULBP1 is the ligand for the NKG2D receptor, together with at least ULBP2 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: NKG2D ligand; NKG2D ligand 1; NKG2D ligand1; UL16-binding protein-like transcript 1; ULBP; ULBP 1
Gene Aliases: A430108B07Rik; MULT1
Entrez Gene ID: (Mouse) 77777
T cell mediated cytotoxicity
antigen processing and presentation of peptide antigen via MHC class I
positive regulation of immune response to tumor cell
natural killer cell activation
positive regulation of interferon-gamma production
positive regulation of natural killer cell activation
natural killer cell mediated cytotoxicity
susceptibility to natural killer cell mediated cytotoxicity
positive regulation of macrophage activation
positive regulation of nitric oxide biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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