Staining of BALB/c splenocytes with isotype control (rat IgG2a purified) (blue histogram) or Rat Anti-Mouse CD4 Purified (clone RM4-5) (left) or Rat Anti-Mouse CD8 (clone 53-6-7) (right) followed by 0.125 µg of F (ab')2 Anti-Rat IgG eFluor® 660 (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

50-4017-82

Applications

Tested Dilution

Flow Cytometry (Flow)

0.25 µg/test

Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat / Ig

Class

Polyclonal

Type

Secondary Antibody

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2574211

Target

IgG

Antibody Form

F(ab')2 Fragment

Product Specific Information

Description: The goat anti-rat IgG polyclonal antibody reacts with the heavy and light chains of rat IgG, with minimal cross reactivity to human, mouse, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to all IgG subclasses: IgG1, IgG2a, IgG2b, IgG2c and some crossreactivity to rat IgM. The antibody is a F(ab')2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding.

This polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a rat IgG followed by this polyclonal antibody).

Applications Reported: This poly antibody has been reported for use in flow cytometric analysis.

Applications Tested: This poly antibody has been tested by flow cytometric analysis of mouse splenocytes first stained with rat anti-mouse antibody. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

F(ab')2-Goat anti-Rat IgG (H+L) Secondary Antibody, eFluor™ 660, eBioscience™

Rat IgG (H+L) Secondary Antibody (50-4017-82) in Flow