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ApoA1 Polyclonal Antibody, PeproTech®

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ApoA1 Polyclonal Antibody, PeproTech®

Product Details

500-P331-100UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.5-2.0 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived, 28.2 kDa Recombinant Human ApoA-I
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930462

Product Specific Information

AA Sequence of recombinant protein: MDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human ApoA-I. Anti-Human ApoA-I-specific antibody was purified by affinity chromatography employing an immobilized Human ApoA-I matrix.

Sandwich ELISA: To detect Human ApoA-I by sandwich ELISA (using 100 µL/well), a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human ApoA-I (500-P331BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human ApoA-I.

Western Blot: To detect Human ApoA-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human ApoA-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 1-Sep; 2-Sep; Apo a; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; Apolipoprotein A-I; Apolipoprotein A1; Apolipoprotein AI; epididymis secretory sperm binding protein; MGC102525; MGC117399; MGC124929; proapo-A-I protein; proapolipoprotein; Udpgt; Ugt1; Ugt1a7; unnamed protein product

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Gene Aliases: AMYLD3; apo(a); APOA1; HPALP2

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UniProt ID: (Human) P02647

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Entrez Gene ID: (Human) 335

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Function(s)
beta-amyloid binding receptor binding lipid transporter activity protein binding phospholipid binding high-density lipoprotein particle binding lipid binding cholesterol binding enzyme binding heat shock protein binding apolipoprotein receptor binding apolipoprotein A-I receptor binding identical protein binding protein homodimerization activity chemorepellent activity receptor agonist activity phosphatidylcholine-sterol O-acyltransferase activator activity high-density lipoprotein particle receptor binding lipoprotein particle binding intermembrane cholesterol transfer activity
Process(es)
endothelial cell proliferation negative regulation of cytokine production involved in immune response lipid metabolic process phospholipid metabolic process phosphatidylcholine biosynthetic process cholesterol biosynthetic process lipid transport transforming growth factor beta receptor signaling pathway G-protein coupled receptor signaling pathway integrin-mediated signaling pathway steroid metabolic process cholesterol metabolic process glucocorticoid metabolic process negative regulation of tumor necrosis factor-mediated signaling pathway positive regulation of cholesterol efflux negative regulation of very-low-density lipoprotein particle remodeling protein oxidation peptidyl-methionine modification lipid storage regulation of intestinal cholesterol absorption cholesterol transport adrenal gland development regulation of Cdc42 protein signal transduction negative regulation of interleukin-1 beta production cholesterol efflux phospholipid efflux negative regulation of heterotypic cell-cell adhesion beta-amyloid formation high-density lipoprotein particle remodeling high-density lipoprotein particle assembly high-density lipoprotein particle clearance positive regulation of Rho protein signal transduction lipoprotein metabolic process lipoprotein biosynthetic process cholesterol homeostasis blood vessel endothelial cell migration reverse cholesterol transport negative regulation of inflammatory response positive regulation of phagocytosis protein stabilization negative chemotaxis vitamin transport positive regulation of stress fiber assembly acylglycerol homeostasis phospholipid homeostasis negative regulation of cell adhesion molecule production negative regulation of response to cytokine stimulus triglyceride homeostasis ERK1 and ERK2 cascade cholesterol import cellular response to lipoprotein particle stimulus positive regulation of cholesterol metabolic process positive regulation of substrate adhesion-dependent cell spreading positive regulation of phospholipid efflux cellular response to beta-amyloid
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