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          • Primary Antibodies ›
          • IL-6 Antibodies

          Invitrogen

          IL-6 Polyclonal Antibody, Biotin, PeproTech®

          View all (213) IL-6 antibodies

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          Cite IL-6 Polyclonal Antibody, Biotin, PeproTech®

          Additional Information:

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          • Antibody Testing Data (3)
          IL-6 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          IL-6 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          IL-6 Antibody (500-P73GBT-25UG) in WB

          Western Blot: To detect Rat IL-6 by Western Blot analysis IL-6 Polyclonal Antibody, Biotin (Product # 500-P73GBT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          IL-6 Antibody in Western Blot (WB)
          IL-6 Antibody in Western Blot (WB)
          IL-6 Antibody in ELISA (ELISA)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          IL-6 Polyclonal Antibody, Biotin, PeproTech®

          Product Details

          500-P73GBT-25UG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.2 µg/mL
          -

          ELISA (ELISA)

          0.25-1.0 µg/mL
          -
          Product Specifications

          Species Reactivity

          Rat

          Host/Isotype

          Goat

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived Recombinant Rat IL-6
          3D Epitope / Immunogen

          Conjugate

          Biotin Biotin Biotin

          Form

          Lyophilized

          Concentration

          0.1-1.0 mg/mL

          Amount

          25 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient

          RRID

          AB_2930735

          Product Specific Information

          AA Sequence of recombinant protein: MFPTSQVRRG DFTEDTTHNR PVYTTSQVGG LITYVLREIL EMRKELCNGN SDCMNSDDAL SENNLKLPEI QRNDGCFQTG YNQEICLLKI CSGLLEFRFY LEFVKNNLQD NKKDKARVIQ SNTETLVHIF KQEIKDSYKI VLPTPTSNAL LMEKLESQKE WLRTKTIQLI LKALEEFLKV TMRSTRQT.

          Preparation: Produced from sera of goats immunized with highly pure Recombinant Rat IL-6. Anti-Rat IL-6-specific antibody was purified by affinity chromatography and then biotinylated.

          Sandwich ELISA: To detect Rat IL-6 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-6 (500-P73G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-6.

          Western Blot: To detect Rat IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

          500-P73GBT-1MG will be provided as 2 x 500 µg

          Target Information

          IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: H-IL-6; IL-6; ILN; Interleukin; Interleukin 6 (interferon, beta 2); Interleukin6; M-IL-6; R-IL-6

          View more View less

          Gene Aliases: Ifnb2; ILg6

          View more View less

          UniProt ID: (Rat) P20607

          View more View less

          Entrez Gene ID: (Rat) 24498

          View more View less

          Function(s)
          receptor binding cytokine activity interleukin-6 receptor binding growth factor activity identical protein binding histone H3K9ac reader activity
          Process(es)
          response to hypoxia neutrophil apoptotic process response to yeast response to amphetamine myeloid cell homeostasis germinal center B cell differentiation hepatic immune response positive regulation of immunoglobulin production positive regulation of acute inflammatory response chromatin organization negative regulation of protein kinase activity response to stress inflammatory response immune response JAK-STAT cascade positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell shape response to xenobiotic stimulus response to wounding response to mechanical stimulus response to bacterium response to gamma radiation gene expression vascular endothelial growth factor production regulation of vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of epithelial to mesenchymal transition positive regulation of neuron projection development response to auditory stimulus positive regulation of gliogenesis response to activity cytokine-mediated signaling pathway cell differentiation response to caffeine endocrine pancreas development neuron projection development lymphocyte costimulation response to nutrient levels cellular response to nutrient levels response to lipopolysaccharide negative regulation of chemokine production positive regulation of chemokine production positive regulation of interleukin-1 beta production positive regulation of interleukin-10 production positive regulation of interleukin-17 production positive regulation of interleukin-21 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production response to insulin negative regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation response to cytokine autocrine signaling cellular response to hepatocyte growth factor stimulus positive regulation of T cell proliferation T cell activation regulation of cell proliferation positive regulation of protein import into nucleus glucose homeostasis defense response to protozoan regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process response to amino acid positive regulation of MAPK cascade negative regulation of neuron apoptotic process cellular response to leptin stimulus regulation of circadian sleep/wake cycle, non-REM sleep positive regulation of nitric oxide biosynthetic process cell redox homeostasis response to ethanol positive regulation of T-helper 2 cell differentiation positive regulation of neuron differentiation negative regulation of gluconeogenesis positive regulation of translation positive regulation of DNA replication negative regulation of bone resorption negative regulation of membrane potential positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade muscle cell cellular homeostasis bone remodeling negative regulation of hormone secretion negative regulation of muscle organ development positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation positive regulation of peptidyl-tyrosine phosphorylation regulation of insulin secretion positive regulation of B cell activation regulation of protein transport positive regulation of lymphocyte activation response to glucocorticoid response to calcium ion response to electrical stimulus defense response to virus positive regulation of protein kinase B signaling positive regulation of transmission of nerve impulse positive regulation of glial cell proliferation branching involved in salivary gland morphogenesis epithelial cell proliferation involved in salivary gland morphogenesis T follicular helper cell differentiation glucagon secretion regulation of glucagon secretion interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to cytokine stimulus cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to estradiol stimulus cellular response to dexamethasone stimulus positive regulation of cell proliferation in bone marrow T-helper 17 cell lineage commitment hepatocyte proliferation positive regulation of extracellular matrix disassembly inflammatory response to wounding cellular response to interleukin-17 liver regeneration receptor signaling pathway via STAT cellular response to virus response to Gram-positive bacterium positive regulation of cytokine production involved in inflammatory response positive regulation of platelet aggregation positive regulation of apoptotic DNA fragmentation positive regulation of pri-miRNA transcription from RNA polymerase II promoter response to resveratrol response to hydrogen sulfide positive regulation of STAT cascade positive regulation of leukocyte adhesion to vascular endothelial cell response to quercetin cellular response to acetaldehyde cellular response to prolactin positive regulation of T-helper 2 cell cytokine production negative regulation of primary miRNA processing negative regulation of interleukin-1-mediated signaling pathway positive regulation of estradiol secretion
          It has to be done as per old AB suggested Products section.

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