Search

  • お問い合わせ一覧​
  • クイックオーダー

Invitrogen

CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16), Super Bright™ 645, eBioscience™

Advanced Verification
12 Published Figures
10 References
View all (101) Integrin beta 1 (CD29) antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}

Product Details

64-0299-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test
View 10 publications 10 publications
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 645, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TS2/16

Conjugate

Super Bright™ 645 Super Bright™ 645 Super Bright™ 645

Excitation/Emission Max

414/645 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2688212

Product Specific Information

Description: The TS2/16 monoclonal antibody reacts with human CD29, also known as integrin beta 1, an approximately 130 kDa single-pass transmembrane glycoprotein. CD29 complexes with one of nine integrin alpha subunits to form the very late antigen (VLA) subfamily of adhesion molecules. Integrin heterodimers containing CD29 are involved in cell-cell and cell-matrix adhesion. CD29 is expressed broadly on lymphocytes and monocytes, with lower levels of expression on granulocytes. The TS2/16 antibody has been found to possess activating activity for beta 1 integrins.

Applications Reported: This TS2/16 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TS2/16 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4 degrees C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD29, also known as ITGB1 or Integrin Subunit Beta 1, is a 110 kDa cell surface glycoprotein widely expressed by various cells, including all leukocytes. It forms non-covalently linked heterodimers with at least six different alpha integrins (CD49a-f), creating VLA-1 through VLA-6 complexes, as well as with CD51. These alpha-beta integrin heterodimers mediate a range of cellular responses, including adhesion, trafficking, proliferation, and differentiation. CD29-containing integrins bind to extracellular matrix proteins such as collagen, laminin, fibronectin, and vitronectin. Some integrins also interact with cellular receptors like VCAM-1 and MadCAM-1. CD29 plays a crucial role in immune cell trafficking from blood to tissue, particularly during leukocyte emigration and extravascular migration. It is involved in regulating tissue damage and disease symptoms related to inflammatory bowel disease. In the presence of anti-tumor drugs, decreased expression of ITGB1 correlates with multidrug resistance in tumor cells. ITGB1-dependent mechanisms enhance cytokine secretion in human airway smooth muscle in response to IL-1beta, facilitated by fibronectin and type I collagen. More than eight beta subunits with numerous splice variant isoforms have been identified in mammals, including two major forms: beta1A and beta1D. Beta1A is present in all tissues except cardiac and skeletal muscle, which express the beta1D variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: CD29; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; integrin beta 1; Integrin beta-1; integrin beta-1 subunit (cytoplasmic domain); Integrin beta1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITBG1D; unnamed protein product; very late activation protein, beta polypeptide; VLA-4 subunit beta

View more View less

Gene Aliases: CD29; FNRB; GPIIA; ITGB1; MDF2; MSK12; VLA-BETA; VLAB

View more View less

UniProt ID: (Human) P05556

View more View less

Entrez Gene ID: (Human) 3688

View more View less

Function(s)
magnesium ion binding virus receptor activity fibronectin binding protease binding actin binding integrin binding calcium ion binding protein binding coreceptor activity protein kinase binding C-X3-C chemokine binding signaling receptor activity laminin binding macromolecular complex binding cadherin binding metal ion binding protein heterodimerization activity cell adhesion molecule binding collagen binding involved in cell-matrix adhesion integrin binding involved in cell-matrix adhesion protein tyrosine kinase binding
Process(es)
G1/S transition of mitotic cell cycle establishment of mitotic spindle orientation angiogenesis in utero embryonic development cell migration involved in sprouting angiogenesis positive regulation of neuroblast proliferation phagocytosis cellular defense response cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion calcium-independent cell-matrix adhesion transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway neuroblast proliferation muscle organ development myoblast fusion cell proliferation germ cell migration visual learning regulation of collagen catabolic process positive regulation of fibroblast migration response to muscle activity cell migration formation of radial glial scaffolds central nervous system neuron differentiation CD40 signaling pathway cell projection organization lamellipodium assembly B cell differentiation extracellular matrix organization positive regulation of cell migration neuron projection development cell-substrate adhesion receptor internalization cell adhesion mediated by integrin cell-cell adhesion mediated by integrin heterotypic cell-cell adhesion negative regulation of Rho protein signal transduction wound healing, spreading of epidermal cells maintenance of blood-brain barrier positive regulation of apoptotic process stress fiber assembly positive regulation of GTPase activity sarcomere organization myoblast differentiation negative regulation of neuron differentiation positive regulation of fibroblast growth factor receptor signaling pathway positive regulation of angiogenesis negative regulation of vasoconstriction viral entry into host cell mesodermal cell differentiation myoblast fate specification axon extension dendrite morphogenesis regulation of developmental process modulation of synaptic transmission leukocyte tethering or rolling regulation of cell cycle positive regulation of protein kinase B signaling positive regulation of glutamate uptake involved in transmission of nerve impulse cardiac muscle cell differentiation cardiac muscle cell myoblast differentiation cardiac cell fate specification regulation of biological quality cellular response to low-density lipoprotein particle stimulus basement membrane organization positive regulation of wound healing cell-cell adhesion regulation of spontaneous synaptic transmission reactive gliosis positive regulation of vascular endothelial growth factor signaling pathway positive regulation of protein localization to plasma membrane regulation of synapse pruning negative regulation of anoikis
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support