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FIGURE: 1 / 9
SOD1 Antibody (702783) in ICC/IF
Knockdown of SOD1 was achieved by transfecting HeLa cells with validated specific siRNA (Silencer® select Product # s451). Immunofluorescence analysis was performed on HeLa cells (untransfected, panel a-d), transfected with SOD1 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labelled with Anti-SOD1 Recombinant Rabbit Monoclonal Antibody (Product # 702783, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate ... View More
Product Details
702783
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
11H3L1
Immunogen
Protein corresponding to human SOD1 (aa1-aa154)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Mouse, Rat
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: amyotrophic lateral sclerosis 1; Amyotrophic lateral sclerosis 1 (ALS1); Cu/Zn SOD; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HGNC:11179; hSod1; Hydrogen sulfide oxidase; indophenoloxidase A; Indophenoloxidase A (IPOA); indophenoloxidase A; soluble; cystolic; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; SOD, soluble; SOD1; superoxide dismutase; Superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); superoxide dismutase 1, soluble; Superoxide dismutase [Cu-Zn]; superoxide dismutase, cystolic; unnamed protein product
Gene Aliases: ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD; SOD1; STAHP
UniProt ID: (Human) P00441
Entrez Gene ID: (Human) 6647
response to reactive oxygen species
response to superoxide
ovarian follicle development
positive regulation of cytokine production
placenta development
retina homeostasis
response to amphetamine
myeloid cell homeostasis
glutathione metabolic process
superoxide metabolic process
cellular iron ion homeostasis
apoptotic process
response to oxidative stress
spermatogenesis
embryo implantation
sensory perception of sound
locomotory behavior
anterograde axonal transport
retrograde axonal transport
regulation of blood pressure
determination of adult lifespan
response to heat
response to xenobiotic stimulus
gene expression
transmission of nerve impulse
neuronal action potential
removal of superoxide radicals
response to nutrient levels
peripheral nervous system myelin maintenance
positive regulation of superoxide anion generation
regulation of T cell differentiation in thymus
response to carbon monoxide
cellular response to oxidative stress
ectopic germ cell programmed cell death
cellular response to potassium ion
regulation of multicellular organism growth
response to hydrogen peroxide
superoxide anion generation
positive regulation of apoptotic process
negative regulation of apoptotic process
regulation of GTPase activity
positive regulation of MAPK cascade
negative regulation of neuron apoptotic process
response to ethanol
regulation of organ growth
response to copper ion
muscle cell cellular homeostasis
thymus development
response to axon injury
hydrogen peroxide biosynthetic process
negative regulation of inflammatory response
positive regulation of phagocytosis
negative regulation of developmental process
regulation of mitochondrial membrane potential
heart contraction
neurofilament cytoskeleton organization
relaxation of vascular smooth muscle
auditory receptor cell stereocilium organization
cellular response to cadmium ion
cellular response to ATP
reactive oxygen species metabolic process
response to antipsychotic drug
action potential initiation
positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
negative regulation of reproductive process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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