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Invitrogen

GATA4 Recombinant Rat Monoclonal Antibody (eBioEvan), Alexa Fluor™ Plus 555

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Product Details

740049TP555

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:200
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50
-

Immunocytochemistry (ICC/IF)

1:100-1:200
-

Flow Cytometry (Flow)

0.03-0.5 µg/test
-

Immunoprecipitation (IP)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

eBioEvan

Conjugate

Alexa Fluor™ Plus 555 Alexa Fluor™ Plus 555 Alexa Fluor™ Plus 555

Excitation/Emission Max

558/572 nm View spectra spectra

Form

Liquid

Concentration

1.0 mg/mL

Amount

50 µg

Purification

Affinity chromatography

Storage buffer

proprietary buffer, pH 6.8

Contains

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_3679162

Product Specific Information

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Applications Tested: This eBioEvan antibody has been tested by western blot, immunocytochemistry and flow cytometric analysis of Hep G2 cells. This may be used for immunocytochemistry at 5 µg/mL and for flow cytometry at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GAT4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; putative; Transcription factor GATA-4; unnamed protein product

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Gene Aliases: ASD2; Gata-4; GATA4; TACHD; TOF; VSD1

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UniProt ID: (Human) P43694, (Mouse) Q08369

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Entrez Gene ID: (Human) 2626, (Mouse) 14463

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding protein kinase binding sequence-specific DNA binding metal ion binding NFAT protein binding RNA polymerase II sequence-specific DNA binding transcription factor binding co-SMAD binding DNA-binding transcription factor binding sequence-specific double-stranded DNA binding transcription factor activity, RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding chromatin binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity transcription factor binding activating transcription factor binding transcription regulatory region DNA binding DNA-binding transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter heart looping atrioventricular node development aortic valve morphogenesis atrioventricular valve formation endocardial cushion development cardiac ventricle morphogenesis cardiac right ventricle morphogenesis ventricular septum development atrial septum primum morphogenesis atrial septum secundum morphogenesis regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cell-cell signaling endoderm development heart development male gonad development response to xenobiotic stimulus response to mechanical stimulus anatomical structure morphogenesis tissue development negative regulation of autophagy positive regulation of vascular endothelial growth factor production negative regulation of cardiac muscle cell apoptotic process cell differentiation positive regulation of BMP signaling pathway response to vitamin A embryonic heart tube anterior/posterior pattern specification atrioventricular canal development wound healing cell fate commitment positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic foregut morphogenesis cardiac muscle cell differentiation transdifferentiation atrial septum morphogenesis intestinal epithelial cell differentiation cardiac muscle tissue regeneration cell growth involved in cardiac muscle cell development cellular response to glucose stimulus regulation of cardiac muscle cell contraction negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of apoptotic signaling pathway in utero embryonic development gastrulation with mouth forming second endoderm formation heart morphogenesis outflow tract morphogenesis atrioventricular valve morphogenesis mitral valve formation tricuspid valve formation ventricular cardiac muscle tissue development cardiac septum development transcription, DNA-templated transcription from RNA polymerase II promoter spermatogenesis regulation of gene expression cardiac muscle hypertrophy in response to stress signal transduction involved in regulation of gene expression BMP signaling pathway response to retinoic acid embryonic heart tube development tube morphogenesis skeletal muscle cell differentiation response to drug response to estrogen embryonic digestive tract morphogenesis embryonic morphogenesis cardiac muscle tissue development Sertoli cell differentiation regulation of cardiac muscle cell proliferation positive regulation of cardiac muscle cell proliferation canonical Wnt signaling pathway SMAD protein signal transduction lung morphogenesis lung lobe formation diaphragm morphogenesis vasculogenesis involved in coronary vascular morphogenesis cellular response to gonadotropin stimulus cellular response to follicle-stimulating hormone stimulus epithelial cell fate commitment seminiferous tubule development
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