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Invitrogen
APEH Polyclonal Antibody
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FIGURE: 1 / 3
APEH Antibody (PA5-96469) in WB
Western blot analysis of APEH in various lysates. Samples were incubated with APEH Polyclonal antibody (Product # PA5-96469) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-96469
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_0016313)
Conjugate
Form
Liquid
Concentration
0.71 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MERQVLLSEP EEAAALYRGL SRQPALSAAC LGPEVTTQYG GQYRTVHTEW TQRDLERMEN IRFCRQYLVF HDGDSVVFAG PAGNSVETRG ELLSRESPSG TMKAVLRKAG GTGPGEEKQF LEVWEKNRKL KSFNLSALEK HGPVYEDDCF GCLSWSHSET HLLYVAEKKR PKAESFFQTK ALDVSASDDE IARLKKPDQA IKGDQFVFYE DWGENMVSKS IPVLCVLDVE SGNISVLEGV PENVSPGQAF WAPGDAGVVF; Positive Samples: SW480, Jurkat, HepG2, Mouse liver, Mouse kidney, Mouse small intestine, Rat bone marrow; Cellular Location: Cytoplasm
Target Information
APEH is the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. APEH gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. The protein can play an important role in destroying oxidatively damaged proteins in living cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AARE; Acyl-peptide hydrolase; Acylamino-acid-releasing enzyme; Acylaminoacyl-peptidase; acylpeptide hydrolase; APH; MGC2178; N-acylaminoacyl peptide hydrolase; N-acylaminoacyl-peptide hydrolase; OPH; Oxidized protein hydrolase
Gene Aliases: AARE; ACPH; APEH; APH; D3F15S2; D3S48E; DNF15S2; OPH
UniProt ID: (Human) P13798, (Mouse) Q8R146, (Rat) P13676
Entrez Gene ID: (Human) 327, (Mouse) 235606, (Rat) 24206
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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