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Invitrogen
ASPH Polyclonal Antibody
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FIGURE: 1 / 5
ASPH Antibody (PA5-78827) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of ASPH in A549 cell using ASPH Polyclonal Antibody (Product # PA5-78827). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 1 µg/mL. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Product Details
PA5-78827
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ASPH (726-758aa EVWQDASSFRLIFIVDVWHPELTPQQRRSLPAI).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Rat Brain Tissue, Rat Liver Tissue, HELA whole cell, HEPG2 whole cell, HEPA whole cell. IHC: Human Mammary Cancer tissue. ICC/IF: A549 cell. Flow: HeLa cell, U87 cell.
Target Information
ASPH is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor-like domains of various proteins. This gene is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor-like domains of various proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: A beta H-J-J; ASP beta-hydroxylase; Aspartate beta-hydroxylase; aspartyl beta-hydroxylase; Aspartyl/asparaginyl beta-hydroxylase; ASPH6; calsequestrin-binding protein; cardiac junctin; Humbug; jumbug; junctate; junctin; junctional sarcoplasmic reticulum protein; junctional sarcoplasmic reticulum protein; calsequestrin binding protein; Peptide-aspartate beta-dioxygenase; unnamed protein product
Gene Aliases: 2310005F16Rik; 3110001L23Rik; AAH; AI848629; ASPH; AW261690; AW561901; BAH; C79816; CASQ2BP1; cI-37; FDLAB; HAAH; JCTN; junctin
UniProt ID: (Human) Q12797, (Mouse) Q8BSY0
Entrez Gene ID: (Human) 444, (Rat) 312981, (Mouse) 65973
structural molecule activity
calcium ion binding
protein binding
structural constituent of muscle
electron carrier activity
oxidoreductase activity
ion channel binding
metal ion binding
dioxygenase activity
peptidyl-aspartic acid 3-dioxygenase activity
molecular_function
peptide-aspartate beta-dioxygenase activity
protein modifying enzyme
detection of calcium ion
muscle contraction
pattern specification process
cell proliferation
negative regulation of cell proliferation
positive regulation of calcium ion transport into cytosol
regulation of cell communication by electrical coupling
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity
regulation of protein stability
activation of store-operated calcium channel activity
response to ATP
limb morphogenesis
positive regulation of proteolysis
positive regulation of transcription, DNA-templated
regulation of cytosolic calcium ion concentration
calcium ion homeostasis
palate development
face morphogenesis
calcium ion transmembrane transport
cellular response to calcium ion
positive regulation of intracellular protein transport
regulation of protein depolymerization
peptidyl-amino acid modification
peptidyl-aspartic acid hydroxylation
activation of cysteine-type endopeptidase activity
oxidation-reduction process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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