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Invitrogen
BSEP Polyclonal Antibody
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FIGURE: 1 / 8
BSEP Antibody (PA5-78690) in IHC (P)
Immunohistochemistry (Paraffin) analysis of BSEP in paraffin-embedded section of mouse liver tissues using BSEP Polyclonal Antibody (Product # PA5-78690). Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 1 µg/mL dilution overn... View More
Product Details
PA5-78690
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ABCB11 (1175-1199aa KYGDNTKEIPMERVIAAAKQAQLHD).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HCCT tissue, rat liver tissue, mouse liver tissue. IHC: Mouse Liver tissue, Rat Liver tissue, Human Liver Cancer tissue.
Target Information
ABCB11 is involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. It is expressed predominatly, if not exclusively, in the liver, where it is further localized to the canilicular microvilli and to subcanilicular vesicles fo the hepatocytes. Structurally, ABCB11 is a multifunctional polypeptide with two homologus halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis 2 (PFIC2). PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ABC member 16, MDR/TAP subfamily; ATP-binding cassette sub-family B member 11; ATP-binding cassette, sub-family B (MDR/TAP), member 11; ATP-binding cassette, sub-family B, member 11; Bile salt export pump; progressive familial intrahepatic cholestasis 2; Sister of P-glycoprotein; sister p-glycoprotein
Gene Aliases: ABC16; ABCB11; BRIC2; BSEP; Lith1; PFIC-2; PFIC2; PGY4; SPGP
UniProt ID: (Human) O95342, (Rat) O70127, (Mouse) Q9QY30
Entrez Gene ID: (Human) 8647, (Rat) 83569, (Mouse) 27413
transporter activity
ATP binding
sodium-exporting ATPase activity, phosphorylative mechanism
canalicular bile acid transmembrane transporter activity
bile acid-exporting ATPase activity
protein binding
drug transmembrane transporter activity
ATPase activity, coupled to transmembrane movement of substances
nucleotide binding
ATPase activity
ATP-binding cassette (ABC) transporter
bile acid biosynthetic process
transport
bile acid and bile salt transport
canalicular bile acid transport
sodium ion transmembrane transport
drug transmembrane transport
response to oxidative stress
metabolic process
response to drug
response to estrogen
drug export
transmembrane transport
anion transmembrane transport
organic acid transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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