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Invitrogen
CD47 Polyclonal Antibody
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FIGURE: 1 / 6
CD47 Antibody (PA5-80435) in ICC/IF
Immunofluorescence staining of CD47 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with CD47 Polyclonal Antibody (Product # PA5-80435, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Product Details
PA5-80435
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human CD47 Protein (Met1-Pro139)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.0
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human CD47.
Target Information
CD47, known as integrin-associated protein (IAP), is a glycosylated transmembrane glycoprotein with five domains, expressed widely across hematopoietic cells like T and B cells, monocytes, platelets, and erythrocytes, as well as non-hematopoietic cells. It interacts with integrins such as CD51/CD61 and CD41/CD61, and serves as a receptor for thrombospondin, mediating bi-directional signaling that affects neural synaptic activity and macrophage phagocytosis. CD47 acts as a ligand for CD172a (SIRP alpha), an inhibitory receptor on macrophages, preventing phagocytosis of CD47-positive cells. This interaction influences cell migration, B cell adhesion, T cell activation, and neuronal development, particularly in synapse-rich brain and retina regions. It also modulates chondrocyte responses to mechanical signals. T cell expression of CD47 can lead to activation or apoptosis in the presence of thrombospondin. Monoclonal antibody stimulation of CD47 has been shown to induce CD4+CD25- suppressive activity and increase Foxp3 expression. CD47's role in membrane transport, signal transduction, and its broad tissue distribution highlight its significance in various physiological processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: antigen identified by monoclonal antibody 1D8; Antigenic surface determinant protein OA3; CD47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; Integrin-associated protein; integrin-associated signal transducer; Leukocyte surface antigen CD47; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47; unnamed protein product
Gene Aliases: CD47; IAP; MER6; OA3
UniProt ID: (Human) Q08722
Entrez Gene ID: (Human) 961
angiogenesis
positive regulation of immune system process
apoptotic process
inflammatory response
cell adhesion
integrin-mediated signaling pathway
positive regulation of cell proliferation
cell migration
positive regulation of cell-cell adhesion
regulation of interferon-gamma production
regulation of interleukin-10 production
regulation of interleukin-12 production
regulation of interleukin-6 production
regulation of tumor necrosis factor production
heterotypic cell-cell adhesion
regulation of nitric oxide biosynthetic process
positive regulation of inflammatory response
negative regulation of phagocytosis
positive regulation of phagocytosis
positive regulation of T cell activation
positive regulation of stress fiber assembly
regulation of Fc receptor mediated stimulatory signaling pathway
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to interleukin-12
cell-cell adhesion
ATP export
positive regulation of monocyte extravasation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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