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CEP290 Polyclonal Antibody

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CEP290 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 22490-1-AP (CEP290 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CEP290 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta using 22490-1-AP (CEP290 antibody) at dilution of 1:50 (under 40x lens).

CEP290 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 22490-1-AP ( CEP290 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

CEP290 Antibody in Immunoprecipitation (IP) — IP result of anti-CEP290 (22490-1-AP for IP and Detection).

Product Details

22490-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 2 publications 2 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CEP290 Fusion Protein Ag17457 (869-1103 aa encoded by BC008641) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.51 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Two isoforms were produced by alternative splicing with predicted MW of 290 and 180 kDa according to UniProt. 22490-1-AP can recognize both.

Immunogen sequence: QMDSDEMKK ILAENSRKIT VLQVNEKSLI RQYTTLVELE RQLRKENEKQ KNELLSMEAE VCEKIGCLQR FKEMAIFKIA ALQKVVDNSV SLSELELANK QYNELTAKYR DILQKDNMLV QRTSNLEHLE CENISLKEQV ESINKELEIT KEKLHTIEQA WEQETKLGNE SSMDKAKKSI TNSDIVSISK KITMLEMKEL NERQRAEHCQ KMYEHLRTSL KQMEERNFEL ETKFAEV (869-1103 aa encoded by BC008641)

Target Information

This gene encodes a protein with 13 putative coiled-coil domains, a region with homology to SMC chromosome segregation ATPases, six KID motifs, three tropomyosin homology domains and an ATP/GTP binding site motif A. The protein is localized to the centrosome and cilia and has sites for N-glycosylation, tyrosine sulfation, phosphorylation, N-myristoylation, and amidation. Mutations in this gene have been associated with Joubert syndrome and nephronophthisis and the presence of antibodies against this protein is associated with several forms of cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A CEP290 C-Terminal Domain Complements the Mutant CEP290 of Rd16 Mice In Trans and Rescues Retinal Degeneration.

Cell reports

Mookherjee S,Chen HY,Isgrig K,Yu W,Hiriyanna S,Levron R,Li T,Colosi P,Chien W,Swaroop A,Wu Z

Tue Oct 16 00:00:00 EDT 2018

The small GTPase RSG1 controls a final step in primary cilia initiation.

The Journal of cell biology

Agbu SO,Liang Y,Liu A,Anderson KV

Tue Jan 02 00:00:00 EST 2018

DAZ-interacting Protein 1 (Dzip1) Phosphorylation by Polo-like Kinase 1 (Plk1) Regulates the Centriolar Satellite Localization of the BBSome Protein during the Cell Cycle.

The Journal of biological chemistry

Zhang B,Wang G,Xu X,Yang S,Zhuang T,Wang G,Ren H,Cheng SY,Jiang Q,Zhang C

Fri Jan 27 00:00:00 EST 2017

DAZ-interacting Protein 1 (Dzip1) Phosphorylation by Polo-like Kinase 1 (Plk1) Regulates the Centriolar Satellite Localization of the BBSome Protein during the Cell Cycle.

The Journal of biological chemistry

Zhang B,Wang G,Xu X,Yang S,Zhuang T,Wang G,Ren H,Cheng SY,Jiang Q,Zhang C

Fri Jan 27 00:00:00 EST 2017

Bioinformatics

Protein Aliases: Bardet-Biedl syndrome 14 protein; Bardet-Biedl syndrome 14 protein homolog; Cancer/testis antigen 87; centrosomal protein 290kDa; Centrosomal protein of 290 kDa; Cep290; CT87; CTCL tumor antigen se2-2; Meckel syndrome, type 4; monoclonal antibody 3H11 antigen; Nephrocystin-6; nephrocytsin-6; POC3 centriolar protein homolog; prostate cancer antigen T21; Tumor antigen se2-2

Gene Aliases: 3H11Ag; b2b1454Clo; b2b1752Clo; BBS14; BC004690; CEP290; CT87; JBTS5; KIAA0373; LCA10; MKS4; NPHP6; POC3; rd16; RGD1311640; SLSN6

UniProt ID: (Human) O15078, (Mouse) Q6A078

Entrez Gene ID: (Human) 80184, (Mouse) 216274, (Rat) 314787

Function(s)

G2/M transition of mitotic cell cycle protein transport hindbrain development otic vesicle formation cilium assembly eye photoreceptor cell development positive regulation of transcription, DNA-templated pronephros development cilium morphogenesis regulation of establishment of protein localization positive regulation of intracellular protein transport transcription, DNA-templated regulation of transcription, DNA-templated transport establishment or maintenance of cell polarity cell projection organization regulation of cAMP metabolic process nonmotile primary cilium assembly photoreceptor cell maintenance retina development in camera-type eye biological_process

Process(es)

protein binding identical protein binding microtubule minus-end binding molecular_function

CEP290 Polyclonal Antibody

Product Details

22490-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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