PA1-069A detects coatomer-protein II (COPII) from rat, mouse and human cells.
PA1-069A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~85 kDa protein representing COPII from PC-12 cell extract.
The PA1-069A immunogen is a synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII. This sequence is completely conserved between human, mouse, and rat. PA1-069A immunizing peptide (Cat. # PEP-127) is available for use in neutralization and control experiments.
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits. COPII consists of three parts: Sar1p and the two protein complexes, Sec23p-Sec24p and Sec13p-Sec31p.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ABP125; ABP130; cop ii; GATOR complex protein SEC13; hSec23A; Protein SEC13 homolog; Protein transport protein Sec23A; Protein transport protein Sec24A; Protein transport protein Sec31A; SEC13 homolog; SEC13 homolog, nuclear pore and COPII coating complex component; SEC13 related; SEC13-like 1; SEC13-like 1 isoform; SEC13-like protein 1; SEC13-related protein; Sec23 homolog A, COPII coat complex component; SEC23-related protein A; SEC23A; SEC24 family member A; SEC24 family, member A; SEC24 related gene family, member A; SEC24-related protein A; SEC31 homolog A, COPII coating complex component; SEC31-like 1; SEC31-like protein 1; SEC31-related protein A; vesicle associated protein; Vesicle-associated protein 1; Web1-like protein; yeast Sec31p homolog
Gene Aliases: 1110003H02Rik; 1810024J13Rik; ABP125; ABP130; AU042160; CLSD; D3S1231E; HSPC275; HSPC334; KIAA0905; Msec23; npp-20; SEC13; SEC13A; SEC13L1; SEC13R; Sec23; SEC23A; Sec23r; SEC24A; SEC31A; SEC31L1; VAP1