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Invitrogen
COPII Polyclonal Antibody
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FIGURE: 1 / 13
COPII Antibody (PA1-069A) in ICC/IF
Immunofluorescent analysis of COP II (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a COP II polyclonal antibody (Product # PA1-069A) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-co... View More
Product Details
PA1-069A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Fruit fly,
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-069A detects coatomer-protein II (COPII) from rat, mouse and human cells.
PA1-069A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~85 kDa protein representing COPII from PC-12 cell extract.
The PA1-069A immunogen is a synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII. This sequence is completely conserved between human, mouse, and rat. PA1-069A immunizing peptide (Cat. # PEP-127) is available for use in neutralization and control experiments.
Target Information
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits. COPII consists of three parts: Sar1p and the two protein complexes, Sec23p-Sec24p and Sec13p-Sec31p.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ABP125; ABP130; cop ii; GATOR2 complex protein SEC13; hSec23A; Protein SEC13 homolog; Protein transport protein Sec23A; Protein transport protein Sec24A; Protein transport protein Sec31A; SEC13 homolog; SEC13 homolog, nuclear pore and COPII coating complex component; SEC13 related; SEC13-like 1; SEC13-like 1 isoform; SEC13-like protein 1; SEC13-related protein; Sec23 homolog A, COPII coat complex component; SEC23-related protein A; SEC23A; SEC24 family member A; SEC24 family, member A; SEC24 related gene family, member A; SEC24-related protein A; SEC31 homolog A, COPII coating complex component; SEC31-like 1; SEC31-like protein 1; SEC31-related protein A; vesicle associated protein; Vesicle-associated protein 1; Web1-like protein; yeast Sec31p homolog
Gene Aliases: 1110003H02Rik; 1810024J13Rik; ABP125; ABP130; AU042160; CLSD; D3S1231E; HSPC275; HSPC334; KIAA0905; Msec23; npp-20; SEC13; SEC13A; SEC13L1; SEC13R; Sec23; SEC23A; Sec23r; SEC24A; SEC31A; SEC31L1; VAP1
UniProt ID: (Human) P55735, (Human) Q15436, (Human) O95486, (Human) O94979, (Mouse) Q9D1M0, (Rat) Q5XFW8, (Mouse) Q01405, (Mouse) Q3U2P1, (Mouse) Q3UPL0, (Rat) Q9Z2Q1
Entrez Gene ID: (Human) 6396, (Human) 10484, (Human) 10802, (Human) 22872, (Mouse) 110379, (Rat) 297522, (Mouse) 20334, (Rat) 58817, (Mouse) 77371, (Rat) 287275, (Mouse) 69162, (Rat) 93646
antigen processing and presentation of peptide antigen via MHC class I
intracellular protein transport
sister chromatid cohesion
antigen processing and presentation of exogenous peptide antigen via MHC class II
positive regulation of TOR signaling
COPII vesicle coating
mRNA transport
ER to Golgi vesicle-mediated transport
vesicle-mediated transport
regulation of low-density lipoprotein particle receptor biosynthetic process
positive regulation of protein secretion
positive regulation of cholesterol homeostasis
protein transport
IRE1-mediated unfolded protein response
response to calcium ion
transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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