Invitrogen

COPII Polyclonal Antibody

8 Published Figures

19 References

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

COPII Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of COP II (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a COP II polyclonal antibody (Product # PA1-069A) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

COPII Antibody in Immunohistochemistry (IHC) — Fig. 2 A Schematic of protein synthesis machinery. Representative 3D confocal images of adult cardiac myocytes showing B ribosomal protein Rpl22 (associated with actively translating ribosomes), C protein transport protein Sec61b, part of the translocon complex, D protein transport protein Sec23a, component of COPII, E trans-Golgi network protein TGN38, and F Cis-Golgi protein GM130. In all cases, proteins are shown in grayscale with nuclei in blue (3-9 cells per protein from 2 hearts). Quantitative analysis of these data and additional control experiments (along with n's for specific experiments) are included in Supplementary Fig. 6. Antibodies used for these studies are listed in the methods

COPII Antibody in Western Blot (WB) — Published figure using COPII polyclonal antibody (Product # PA1-069A) in Western Blot

Product Details

PA1-069A

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:200

View 10 publications 10 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Functional Assay (FN)

View 1 publication 1 publication

Immunomicroscopy (IM)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Fruit fly, Hamster, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2301581

Product Specific Information

PA1-069A detects coatomer-protein II (COPII) from rat, mouse and human cells.

PA1-069A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~85 kDa protein representing COPII from PC-12 cell extract.

The PA1-069A immunogen is a synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII. This sequence is completely conserved between human, mouse, and rat. PA1-069A immunizing peptide (Cat. # PEP-127) is available for use in neutralization and control experiments.

Target Information

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits. COPII consists of three parts: Sar1p and the two protein complexes, Sec23p-Sec24p and Sec13p-Sec31p.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deficiency of Perry syndrome-associated p150^Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.

NPJ Parkinson's disease

Yu J,Yang X,Zheng J,Sgobio C,Sun L,Cai H

PA1-069A was used in Western Blotting to demonstrate the importance of p150Glued in controlling the structure and function of ER, which is critical for the survival and function of midbrain DAergic neurons in PS. © 2023.

Tue Mar 07 00:00:00 EST 2023

ESCRT dysfunction compromises endoplasmic reticulum maturation and autophagosome biogenesis in Drosophila.

Current biology : CB

Wang R,Miao G,Shen JL,Fortier TM,Baehrecke EH

PA1-069A was used in Immunomicroscopy to reveal a novel ESCRT-dependent pathway that influences the ERGIC and autophagosome formation.

Mon Mar 28 00:00:00 EDT 2022

Neuroprogenitor Cells From Patients With TBCK Encephalopathy Suggest Deregulation of Early Secretory Vesicle Transport.

Frontiers in cellular neuroscience

Moreira DP,Suzuki AM,Silva ALTE,Varella-Branco E,Meneghetti MCZ,Kobayashi GS,Fogo M,Ferrari MFR,Cardoso RR,Lourenço NCV,Griesi-Oliveira K,Zachi EC,Bertola DR,Weinmann KS,de Lima MA,Nader HB,Sertié AL,Passos-Bueno MR

PA1-069A was used in Immunocytochemistry to gain insights into mechanisms of action of TBC1 domain containing kinase (TBCK) in neural cells and to better understand the pathophysiology of infantile hypotonia with psychomotor retardation, and characteristic facies 3 (IHPRF3) syndrome.

Tue Feb 01 00:00:00 EST 2022

A common human missense mutation of vesicle coat protein SEC23B leads to growth restriction and chronic pancreatitis in mice.

The Journal of biological chemistry

Wei W,Liu Z,Zhang C,Khoriaty R,Zhu M,Zhang B

PA1-069A was used in Functional Assay to conclude that the Sec23bki/ko mice provide a novel model of chronic pancreatitis and growth retardation with GH insensitivity.

Sat Jan 01 00:00:00 EST 2022

Imaging of type I procollagen biosynthesis in cells reveals biogenesis in highly organized bodies; Collagenosomes.

Matrix biology plus

Stefanovic B,Stefanovic L,Manojlovic Z

PA1-069A was used in Immunocytochemistry to report on the structural organization of type I collagen biogenesis in collagenosomes.

Wed Dec 01 00:00:00 EST 2021

Distributed synthesis of sarcolemmal and sarcoplasmic reticulum membrane proteins in cardiac myocytes.

Basic research in cardiology

Bogdanov V,Soltisz AM,Moise N,Sakuta G,Orengo BH,Janssen PML,Weinberg SH,Davis JP,Veeraraghavan R,Györke S

PA1-069A was used in Immunohistochemistry (PFA fixed) to identify previously unanticipated aspects of local control of cardiac myocyte biology and highlight local protein synthesis in cardiac myocytes as an important potential determinant of the heart's biology in health and disease.

Thu Oct 28 00:00:00 EDT 2021

Distributed synthesis of sarcolemmal and sarcoplasmic reticulum membrane proteins in cardiac myocytes.

Basic research in cardiology

Bogdanov V,Soltisz AM,Moise N,Sakuta G,Orengo BH,Janssen PML,Weinberg SH,Davis JP,Veeraraghavan R,Györke S

Thu Oct 28 00:00:00 EDT 2021

Tango1 spatially organizes ER exit sites to control ER export.

The Journal of cell biology

Liu M,Feng Z,Ke H,Liu Y,Sun T,Dai J,Cui W,Pastor-Pareja JC

Mon Apr 03 00:00:00 EDT 2017

Tango1 spatially organizes ER exit sites to control ER export.

The Journal of cell biology

Liu M,Feng Z,Ke H,Liu Y,Sun T,Dai J,Cui W,Pastor-Pareja JC

Mon Apr 03 00:00:00 EDT 2017

α-Synuclein-induced lysosomal dysfunction occurs through disruptions in protein trafficking in human midbrain synucleinopathy models.

Proceedings of the National Academy of Sciences of the United States of America

Mazzulli JR,Zunke F,Isacson O,Studer L,Krainc D

Published figure using COPII polyclonal antibody (Product # PA1-069A) in Immunocytochemistry

Tue Feb 16 00:00:00 EST 2016

A stress assembly that confers cell viability by preserving ERES components during amino-acid starvation.

eLife

Zacharogianni M,Aguilera-Gomez A,Veenendaal T,Smout J,Rabouille C

PA1-069A was used in immunocytochemistry and western blot to study a stress assembly that supports cell viability by preserving ERES components during amino-acid starvation

Tue Nov 11 00:00:00 EST 2014

A stress assembly that confers cell viability by preserving ERES components during amino-acid starvation.

eLife

Zacharogianni M,Aguilera-Gomez A,Veenendaal T,Smout J,Rabouille C

PA1-069A was used in immunocytochemistry and western blot to study a stress assembly that supports cell viability by preserving ERES components during amino-acid starvation

Tue Nov 11 00:00:00 EST 2014

Two mammalian Sec16 homologues have nonredundant functions in endoplasmic reticulum (ER) export and transitional ER organization.

Molecular biology of the cell

Bhattacharyya D,Glick BS

Published figure using COPII polyclonal antibody (Product # PA1-069A) in Immunofluorescence

Thu Mar 01 00:00:00 EST 2007

On and off membrane dynamics of the endoplasmic reticulum-golgi tethering factor p115 in vivo.

Molecular biology of the cell

Brandon E,Szul T,Alvarez C,Grabski R,Benjamin R,Kawai R,Sztul E

PA1-069A was used in immunocytochemistry to investigate the mechanism for membrane recruitment of the p115 tethering factor.

Sat Jul 01 00:00:00 EDT 2006

Cholesterol is required for efficient endoplasmic reticulum-to-Golgi transport of secretory membrane proteins.

Molecular biology of the cell

Ridsdale A,Denis M,Gougeon PY,Ngsee JK,Presley JF,Zha X

PA1-069A was used in immunocytochemistry to investigate the role of cholesterol during endoplasmic reticulum-to-Golgi transport.

Sat Apr 01 00:00:00 EST 2006

Golgi inheritance in mammalian cells is mediated through endoplasmic reticulum export activities.

Molecular biology of the cell

Altan-Bonnet N,Sougrat R,Liu W,Snapp EL,Ward T,Lippincott-Schwartz J

PA1-069A was used in immunocytochemistry to investigate the ER-dependent model of Golgi inheritance in mammalian cells.

Wed Feb 01 00:00:00 EST 2006

Biogenesis of tubular ER-to-Golgi transport intermediates.

Molecular biology of the cell

Simpson JC,Nilsson T,Pepperkok R

PA1-069A was used in western blot to investigate the roles of intact microtubules during the formation and motility of Tubular transport intermediates.

Wed Feb 01 00:00:00 EST 2006

Cis-Golgi matrix proteins move directly to endoplasmic reticulum exit sites by association with tubules.

Molecular biology of the cell

Mardones GA,Snyder CM,Howell KE

PA1-069A was used in immunocytochemistry to demonstrate the role of Golgi cis-medial matrix proteins in Golgi-to-ER traffic and in tubule formation and targeting.

Sun Jan 01 00:00:00 EST 2006

Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion.

The EMBO journal

Nakajima K,Hirose H,Taniguchi M,Kurashina H,Arasaki K,Nagahama M,Tani K,Yamamoto A,Tagaya M

PA1-069A was used in immunoprecipitation to investigate the role of the alpha-SNAP during the apoptosis.

Wed Aug 18 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: ABP125; ABP130; cop ii; GATOR complex protein SEC13; hSec23A; Protein SEC13 homolog; Protein transport protein Sec23A; Protein transport protein Sec24A; Protein transport protein Sec31A; SEC13 homolog; SEC13 homolog, nuclear pore and COPII coating complex component; SEC13 related; SEC13-like 1; SEC13-like 1 isoform; SEC13-like protein 1; SEC13-related protein; Sec23 homolog A, COPII coat complex component; SEC23-related protein A; SEC23A; SEC24 family member A; SEC24 family, member A; SEC24 related gene family, member A; SEC24-related protein A; SEC31 homolog A, COPII coating complex component; SEC31-like 1; SEC31-like protein 1; SEC31-related protein A; vesicle associated protein; Vesicle-associated protein 1; Web1-like protein; yeast Sec31p homolog

Gene Aliases: 1110003H02Rik; 1810024J13Rik; ABP125; ABP130; AU042160; CLSD; D3S1231E; HSPC275; HSPC334; KIAA0905; Msec23; npp-20; SEC13; SEC13A; SEC13L1; SEC13R; Sec23; SEC23A; Sec23r; SEC24A; SEC31A; SEC31L1; VAP1

UniProt ID: (Human) P55735, (Human) Q15436, (Human) O95486, (Human) O94979, (Mouse) Q9D1M0, (Rat) Q5XFW8, (Mouse) Q01405, (Mouse) Q3U2P1, (Mouse) Q3UPL0, (Rat) Q9Z2Q1