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Invitrogen
COPII Polyclonal Antibody
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FIGURE: 1 / 13
COPII Antibody (PA1-069A) in ICC/IF
Immunofluorescent analysis of COP II (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a COP II polyclonal antibody (Product # PA1-069A) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-co... View More
Product Details
PA1-069A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Fruit fly,
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-069A detects coatomer-protein II (COPII) from rat, mouse and human cells.
PA1-069A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~85 kDa protein representing COPII from PC-12 cell extract.
The PA1-069A immunogen is a synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII. This sequence is completely conserved between human, mouse, and rat. PA1-069A immunizing peptide (Cat. # PEP-127) is available for use in neutralization and control experiments.
Target Information
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits. COPII consists of three parts: Sar1p and the two protein complexes, Sec23p-Sec24p and Sec13p-Sec31p.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ABP125; ABP130; cop ii; GATOR complex protein SEC13; GATOR2 complex protein SEC13; hSec23A; Protein SEC13 homolog; Protein transport protein Sec23A; Protein transport protein Sec24A; Protein transport protein Sec31A; SEC13 homolog, nuclear pore and COPII coating complex component; SEC13 related; SEC13-like 1; SEC13-like protein 1; SEC13-related protein; Sec23 homolog A, coat complex II component; SEC23-related protein A; SEC23A; SEC24 family, member A; SEC24 related gene family, member A; SEC24-related protein A; SEC31 homolog A, COPII coating complex component; SEC31-like 1; SEC31-like protein 1; SEC31-related protein A; unnamed protein product; vesicle associated protein; Vesicle-associated protein 1; Web1-like protein; yeast Sec31p homolog
Gene Aliases: 1110003H02Rik; 1810024J13Rik; ABP125; ABP130; AU042160; CLSD; D3S1231E; HPBKS; hSec23A; HSPC275; HSPC334; KIAA0905; Msec23; NEDSOSB; npp-20; SEC13; SEC13A; SEC13L1; SEC13R; Sec23; SEC23A; Sec23r; SEC24A; SEC31A; SEC31L1; VAP1
UniProt ID: (Human) P55735, (Human) Q15436, (Human) O95486, (Human) O94979, (Mouse) Q9D1M0, (Rat) Q5XFW8, (Mouse) Q01405, (Mouse) Q3U2P1, (Mouse) Q3UPL0, (Rat) Q9Z2Q1
Entrez Gene ID: (Human) 6396, (Human) 10484, (Human) 10802, (Human) 22872, (Mouse) 110379, (Rat) 297522, (Mouse) 20334, (Rat) 58817, (Mouse) 77371, (Rat) 287275, (Mouse) 69162, (Rat) 93646
protein import into nucleus
intracellular protein transport
nucleocytoplasmic transport
protein transport
vesicle-mediated transport
cellular response to nutrient levels
positive regulation of TOR signaling
protein exit from endoplasmic reticulum
mRNA transport
cargo loading into COPII-coated vesicle
COPII-coated vesicle budding
negative regulation of TORC1 signaling
positive regulation of TORC1 signaling
ER to Golgi vesicle-mediated transport
protein localization to plasma membrane
regulation of cholesterol transport
cholesterol homeostasis
positive regulation of protein secretion
endoplasmic reticulum organization
response to calcium ion
transport
regulation of low-density lipoprotein particle receptor biosynthetic process
positive regulation of cholesterol homeostasis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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