|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) G L E T E K A D V Q L F M A D D A Y(19) C of rat CAV2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-065 detects caveolin-2 from rat and mouse tissues tissues. This antibody does not detect caveolin-1 or -3.
PA1-065 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a ~21 kDa protein representing caveolin-2 from rat heart homogenate.
The PA1-065 immunogen is a synthetic peptide corresponding to residues M(1) G L E T E K A D V Q L F M A D D A Y(19) C of rat CAV2. This sequence is completely conserved in mouse, and ~90% conserved in human caveolin-2. The PA1-065 immunizing peptide (Cat. # PEP-080) is available for use in neutralization and control experiments.
Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Intracellular retention of glycosylphosphatidyl inositol-linked proteins in caveolin-deficient cells.
PA1-065 was used in western blot to investigate the effect of caveolin on glycosylphosphatidyl inositol-linked protein function .
|Sotgia F,Razani B,Bonuccelli G,Schubert W,Battista M,Lee H,Capozza F,Schubert AL,Minetti C,Buckley JT,Lisanti MP||Molecular and cellular biology (22:3905)||2002|