|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 19 amino acid peptide near the carboxy terminus of human DAP|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||5 ug/ml|
|Western Blot (WB)||1 - 2 ug/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is human small intestine lysate.
PA5-34564 can be used with blocking peptide PEP-1607.
The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma. DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy .
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.