Invitrogen

DHTKD1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

2 References

View all (9) DHTKD1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

DHTKD1 Antibody in Western Blot (WB) — Western blot analysis using a DHTKD1 polyclonal antibody (Product # PA5-24208) in mouse liver tissue lysates (35 µg per lane).

DHTKD1 Antibody — Figure 6 MGF induces Dhtkd1 and Cox6b, and suppresses Acac and Scd1 in hepatic tissues and cells. (A) Western blots of Dhtkd1, Cox6b1, Acac and Scd1 in proteins extracted from liver tissues from C57BL6/J mice fed with HFD +- MGF for 18 weeks. (B) Quantification of protein expression in (A). Liver tissues were from 2 groups (HFD +- MGF), 3 mice per group. Values are average +- SEM (n = 3). (C) Western blots of Dhtkd1, Cox6b1, Acac and Scd1 in proteins extracted from mouse primary hepatocytes and treated with or without 200 uM MGF for 24 hours. (D) Quantification of protein expression in (C). Values are average +- SEM (n = 3). *p<0.05, **p<0.01 by two tail t-test. {TM}

Product Details

PA5-24208

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Rabbit / Ig
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 392-421 amino acids from the central region of human DHTKD1 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2541708

Product Specific Information

This antibody is predicted to react with rat based on sequence homology.

Target Information

The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Synthetic analogues of 2-oxo acids discriminate metabolic contribution of the 2-oxoglutarate and 2-oxoadipate dehydrogenases in mammalian cells and tissues.

Scientific reports

Artiukhov AV,Grabarska A,Gumbarewicz E,Aleshin VA,Kähne T,Obata T,Kazantsev AV,Lukashev NV,Stepulak A,Fernie AR,Bunik VI

Published figure using DHTKD1 polyclonal antibody (Product # PA5-24208) in Western Blot

Wed Feb 05 00:00:00 EST 2020

Dual mode action of mangiferin in mouse liver under high fat diet.

PloS one

Lim J,Liu Z,Apontes P,Feng D,Pessin JE,Sauve AA,Angeletti RH,Chi Y

PA5-24208 was used in western blot to study the mechanisms underlying the beneficial effects of mangiferin against the metabolic derrangements induced by a high-fat diet

Mon Jan 05 00:00:00 EST 2015

Bioinformatics

Protein Aliases: Dehydrogenase E1 and transketolase domain-containing protein 1; DKFZp762M115; MGC3090; Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial

Gene Aliases: AMOXAD; C330018I04Rik; CMT2Q; DHTKD1; KIAA1630

UniProt ID: (Human) Q96HY7, (Mouse) A2ATU0

Entrez Gene ID: (Human) 55526, (Mouse) 209692

Molecular Function: dehydrogenase