Invitrogen

DHTKD1 Polyclonal Antibody

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DHTKD1 Antibody in Western Blot (WB) — Western blot analysis using a DHTKD1 polyclonal antibody (Product # PA5-24208) in mouse liver tissue lysates (35 µg per lane).

DHTKD1 Antibody — Figure 6 MGF induces Dhtkd1 and Cox6b, and suppresses Acac and Scd1 in hepatic tissues and cells. (A) Western blots of Dhtkd1, Cox6b1, Acac and Scd1 in proteins extracted from liver tissues from C57BL6/J mice fed with HFD +- MGF for 18 weeks. (B) Quantification of protein expression in (A). Liver tissues were from 2 groups (HFD +- MGF), 3 mice per group. Values are average +- SEM (n = 3). (C) Western blots of Dhtkd1, Cox6b1, Acac and Scd1 in proteins extracted from mouse primary hepatocytes and treated with or without 200 uM MGF for 24 hours. (D) Quantification of protein expression in (C). Values are average +- SEM (n = 3). *p<0.05, **p<0.01 by two tail t-test.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 392-421 amino acids from the central region of human DHTKD1

Conjugate

Unconjugated

Form

Liquid

Concentration

Lot-specific

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2541708

Product Specific Information

This antibody is predicted to react with rat based on sequence homology.

Target Information

The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Dehydrogenase E1 and transketolase domain-containing protein 1; DKFZp762M115; MGC3090; Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial

Gene Aliases: AMOXAD; C330018I04Rik; CMT2Q; DHTKD1; KIAA1630

UniProt ID: (Human) Q96HY7, (Mouse) A2ATU0

Entrez Gene ID: (Human) 55526, (Mouse) 209692

Function(s)

hematopoietic progenitor cell differentiation generation of precursor metabolites and energy glycolytic process tricarboxylic acid cycle glyoxylate metabolic process metabolic process oxidation-reduction process

Process(es)

oxoglutarate dehydrogenase (succinyl-transferring) activity thiamine pyrophosphate binding molecular_function oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor