Invitrogen

DNAJB9 Polyclonal Antibody

View all (8) DNAJB9 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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DNAJB9 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of DNAJB9 in A431 cells using DNAJB9 Polyclonal Antibody (Product # PA5-142776). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

DNAJB9 Antibody in Western Blot (WB) — Western Blot analysis of DNAJB9 in Human Liver (A), Kidney (B) and Mouse Liver (C) lysate (35 µg protein in RIPA buffer) using DNAJB9 Polyclonal Antibody (Product # PA5-142776) at 1 µg/mL. Detected by chemiluminescence.

DNAJB9 Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of DNAJB9 in A431 cells (blue line) using DNAJB9 Polyclonal Antibody (Product # PA5-142776). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Product Details

PA5-142776

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	-
Immunocytochemistry (ICC/IF)	10 µg/mL	-
Flow Cytometry (Flow)	10 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide with sequence C-PDAEAKFREIAEAYE. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:16,000.

Target Information

This gene is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. This gene is a member of the type 2 subgroup of DnaJ proteins. The encoded protein is localized to the endoplasmic reticulum. This protein is induced by endoplasmic reticulum stress and plays a role in protecting stressed cells from apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZP564F1862; DNA j protein b9; DnaJ (Hsp40) homolog, subfamily B, member 9; DnaJ homolog subfamily B member 9; Endoplasmic reticulum DNA J domain-containing protein 4; endoplasmic reticulum DnaJ homolog 4; ER-resident protein ERdj4; Mdg-1; mDj7; microvascular endothelial differentiation gene 1; Microvascular endothelial differentiation gene 1 protein

Gene Aliases: AA408011; AA673251; AA673481; AW556981; DNAJB9; ERdj4; MDG-1; MDG1; mDj7; MST049; MSTP049; UNQ743/PRO1471

UniProt ID: (Human) Q9UBS3, (Mouse) Q9QYI6

Entrez Gene ID: (Human) 4189, (Mouse) 27362

Molecular Function: chaperone