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DOT1L Polyclonal Antibody
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Product Details
39953
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This DOT1L antibody was raised against a peptide derived from the C-terminal region of human DOT1L.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Purification
Antigen affinity chromatography, Affinity chromatography
Storage buffer
70mM tris, pH 8, with 30% glycerol, 0.07mM EDTA, 105mM NaCl, 31mM glycine
Contains
0.035% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
DOT1-like histone lysine methyltransferase (DOT1L) is a gene located on chromosome 19q13.12, encoding an enzyme responsible for methylating lysine 79 on histone H3, a critical modification influencing chromatin structure and gene expression. Unlike most histone methyltransferases, DOT1L does not contain a SET domain and uniquely catalyzes H3K79 methylation, which plays pivotal roles in the regulation of transcription and maintenance of genomic stability. Expression of DOT1L is vital for various biological processes, including cell cycle progression, development, and differentiation, particularly in hematopoietic cells. Aberrations in DOT1L activity have been associated with mixed-lineage leukemia (MLL), where DOT1L-mediated H3K79 methylation contributes to leukemogenesis by influencing gene expression related to proliferation and survival. Due to its involvement in cancer progression, DOT1L is being investigated as a therapeutic target, with inhibitors developed to specifically modulate its enzymatic activity, offering potential for treating MLL and other malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Disruptor of telomeric silencing 1-like histone lysine methyltransferase; DKFZp586P1823; DOT1 like histone H3K79 methyltransferase; DOT1-like histone methyltransferase; DOT1-like protein; DOT1-like, histone H3 methyltransferase; H3-K79-HMTase; Histone H3-K79 methyltransferase; histone methyltransferase; histone methyltransferase DOT1L; Histone-lysine N-methyltransferase, H3 lysine-79 specific; Lysine N-methyltransferase 4
Gene Aliases: DOT1; DOT1L; KIAA1814; KMT4
UniProt ID: (Human) Q8TEK3
Entrez Gene ID: (Human) 84444
nucleic acid binding
DNA binding
transcription coactivator activity
protein binding
methyltransferase activity
transferase activity
histone methyltransferase activity (H3-K79 specific)
histone methyltransferase activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
histone H3 methyltransferase activity
histone H3K79 trimethyltransferase activity
DNA damage checkpoint
DNA repair
chromatin organization
gene expression
heterochromatin assembly
telomeric heterochromatin assembly
telomere organization
methylation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of JAK-STAT cascade
regulation of cell cycle
regulation of transcription regulatory region DNA binding
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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