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Invitrogen
Dectin-1 Polyclonal Antibody
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FIGURE: 1 / 1
Dectin-1 Antibody (PA5-97284) in WB
Western blot analysis of Dectin-1 in extracts of various cell lines. Samples were incubated with Dectin-1 Polyclonal antibody (Product # PA5-97284) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-97284
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 140-240 of human CLEC7A (NP_922938.1).
Conjugate
Form
Liquid
Concentration
1.61 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: SWDGSKRQCW QLGSNLLKID SSNELGFIVK QVSSQPDNSF WIGLSRPQTE VPWLWEDGST FSSNLFQIRT TATQENPSPN CVWIHVSVIY DQLCSVPSYS I
Positive Samples: H-460, SGC-7901, HL-60, Mouse spleen, Mouse thymus, Mouse liver, Mouse lung, Rat spleen; Cellular Location: Cell membrane, Cytoplasm, Single-pass type II membrane protein
Target Information
CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta-glucan receptor; C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 12; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; CD369; DC-associated C-type lectin 1; dectin-1; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; lectin-like receptor 1
Gene Aliases: beta-GR; BGR; CANDF4; CD369; CLEC7A; Clec7b; CLECSF12; DECTIN1; RGD1565140; SCARE2; UNQ539/PRO1082
UniProt ID: (Human) Q9BXN2
Entrez Gene ID: (Human) 64581, (Mouse) 56644, (Rat) 502902
response to yeast
detection of yeast
pattern recognition receptor signaling pathway
response to molecule of fungal origin
leukocyte activation involved in immune response
immune system process
cell surface pattern recognition receptor signaling pathway
phagocytosis, recognition
phagocytosis, engulfment
inflammatory response
cell recognition
single organismal cell-cell adhesion
positive regulation of tumor necrosis factor production
innate immune response
positive regulation of phagocytosis
cellular response to molecule of fungal origin
stimulatory C-type lectin receptor signaling pathway
carbohydrate mediated signaling
T cell activation
defense response to protozoan
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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