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EIF3D Polyclonal Antibody

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Antibody Testing Data (18)

EIF3D Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

EIF3D Antibody (10219-1-AP) in ICC/IF

IF result of 10219-1-AP (anti-EIF3D) in U2OS cell (treated with 100 mM sodium arsenite to cause stress-induced translational arrest) by Dr. Nancy Kedersha.U2OS cells (FAST-YFP stables; but not showing FAST-YFP); stained with PTG anti-eIF3p66 in green; and counterstained with anti-eIF3b (goat polyclonal) in red; nuclei stained blue using Hoechst.

Product Image Gallery

EIF3D Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10219-1-AP ( EIF3D Antibody) at dilution of 1:50 (under 40x lens).

EIF3D Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10219-1-AP ( EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

EIF3D Antibody in Immunoprecipitation (IP) — IP result of anti-EIF3D (IP:10219-1-AP, 4ug; Detection:10219-1-AP 1:300) with A549 cells lysate 2800 ug.

Product Details

10219-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Yeast

Published species

Human, Mouse, Rabbit, Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EIF3D Fusion Protein Ag0268 (323-531 aa encoded by BC000328)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: FSQQCLRMG KERYNFPNPN PFVEDDMDKN EIASVAYRYR RWKLGDDIDL IVRCEHDGVM TGANGEVSFI NIKTLNEWDS RHCNGVDWRQ KLDSQRGAVI ATELKNNSYK LARWTCCALL AGSEYLKLGY VSRYHVKDSS RHVILGTQQF KPNEFASQIN LSVENAWGIL RCVIDICMKL EEGKYLILKD PNKQVIRVYS LPDGTFSSDE (323-531 aa encoded by BC000328)

Target Information

eIF3D is a protein belonging to the largest of the eukaryotic translation initiation factor family, eukaryotic translation initiation factor-3 (eIF3). eIF3 is a multiprotein complex composed of at least 13 different subunits. eIF3D binds to the 40S ribosomal subunit and thus helps in maintaining the 40S and 60S ribosomal subunits in a dissociated state. An important role of eIF3D is the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and promotes mRNA binding. eIF3D is the major RNA binding subunit of the eIF3 complex. It associates with the subunit p170 of eIF-3. Ubiquitous expression of eIF3D is seen in most of the tissues and it is phosphorylated upon DNA damage, likely by ATM or ATR.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules.

Cell

Cui Q,Bi H,Lv Z,Wu Q,Hua J,Gu B,Huo C,Tang M,Chen Y,Chen C,Chen S,Zhang X,Wu Z,Lao Z,Sheng N,Shen C,Zhang Y,Wu ZY,Jin Z,Yang P,Liu H,Li J,Bai G

Thu Feb 16 00:00:00 EST 2023

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

iScience

Iwasaki M,Kawahara Y,Okubo C,Yamakawa T,Nakamura M,Tabata T,Nishi Y,Narita M,Ohta A,Saito H,Yamamoto T,Nakagawa M,Yamanaka S,Takahashi K

Fri May 20 00:00:00 EDT 2022

An eIF3d-dependent switch regulates HCMV replication by remodeling the infected cell translation landscape to mimic chronic ER stress.

Cell reports

Thompson L,Depledge DP,Burgess HM,Mohr I

Tue May 03 00:00:00 EDT 2022

Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells.

iScience

Yu NK,McClatchy DB,Diedrich JK,Romero S,Choi JH,Martínez-Bartolomé S,Delahunty CM,Muotri AR,Yates JR

Fri Nov 19 00:00:00 EST 2021

Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells.

iScience

Yu NK,McClatchy DB,Diedrich JK,Romero S,Choi JH,Martínez-Bartolomé S,Delahunty CM,Muotri AR,Yates JR

Fri Nov 19 00:00:00 EST 2021

Controlling tissue patterning by translational regulation of signaling transcripts through the core translation factor eIF3c.

Developmental cell

Fujii K,Zhulyn O,Byeon GW,Genuth NR,Kerr CH,Walsh EM,Barna M

Mon Nov 08 00:00:00 EST 2021

G3BP1 interacts with YWHAZ to regulate chemoresistance and predict adjuvant chemotherapy benefit in gastric cancer.

British journal of cancer

Zhao J,Fu X,Chen H,Min L,Sun J,Yin J,Guo J,Li H,Tang Z,Ruan Y,Wang X,Sun Y,Huang L

Fri Jan 01 00:00:00 EST 2021

A translational program that suppresses metabolism to shield the genome.

Nature communications

Balukoff NC,Ho JJD,Theodoridis PR,Wang M,Bokros M,Llanio LM,Krieger JR,Schatz JH,Lee S

Fri Nov 13 00:00:00 EST 2020

EIF3D promotes sunitinib resistance of renal cell carcinoma by interacting with GRP78 and inhibiting its degradation.

EBioMedicine

Huang H,Gao Y,Liu A,Yang X,Huang F,Xu L,Danfeng X,Chen L

Fri Nov 01 00:00:00 EDT 2019

Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.

Cell death & disease

Aulas A,Lyons SM,Fay MM,Anderson P,Ivanov P

Tue Nov 13 00:00:00 EST 2018

Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide.

Molecular cell

Shi Z,Fujii K,Kovary KM,Genuth NR,Röst HL,Teruel MN,Barna M

Thu Jul 06 00:00:00 EDT 2017

Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide.

Molecular cell

Shi Z,Fujii K,Kovary KM,Genuth NR,Röst HL,Teruel MN,Barna M

Thu Jul 06 00:00:00 EDT 2017

Proteomics analysis of bladder cancer invasion: Targeting EIF3D for therapeutic intervention.

Oncotarget

Latosinska A,Mokou M,Makridakis M,Mullen W,Zoidakis J,Lygirou V,Frantzi M,Katafigiotis I,Stravodimos K,Hupe MC,Dobrzynski M,Kolch W,Merseburger AS,Mischak H,Roubelakis MG,Vlahou A

Tue Sep 19 00:00:00 EDT 2017

A Conserved Interaction between a C-Terminal Motif in Norovirus VPg and the HEAT-1 Domain of eIF4G Is Essential for Translation Initiation.

PLoS pathogens

Leen EN,Sorgeloos F,Correia S,Chaudhry Y,Cannac F,Pastore C,Xu Y,Graham SC,Matthews SJ,Goodfellow IG,Curry S

Fri Jan 01 00:00:00 EST 2016

A Conserved Interaction between a C-Terminal Motif in Norovirus VPg and the HEAT-1 Domain of eIF4G Is Essential for Translation Initiation.

PLoS pathogens

Leen EN,Sorgeloos F,Correia S,Chaudhry Y,Cannac F,Pastore C,Xu Y,Graham SC,Matthews SJ,Goodfellow IG,Curry S

Fri Jan 01 00:00:00 EST 2016

The LIF-mediated molecular signature regulating murine embryo implantation.

Biology of reproduction

Rosario GX,Hondo E,Jeong JW,Mutalif R,Ye X,Yee LX,Stewart CL

Mon Sep 01 00:00:00 EDT 2014

The LIF-mediated molecular signature regulating murine embryo implantation.

Biology of reproduction

Rosario GX,Hondo E,Jeong JW,Mutalif R,Ye X,Yee LX,Stewart CL

Mon Sep 01 00:00:00 EDT 2014

Int6 regulates both proteasomal degradation and translation initiation and is critical for proper formation of acini by human mammary epithelium.

Oncogene

Suo J,Snider SJ,Mills GB,Creighton CJ,Chen AC,Schiff R,Lloyd RE,Chang EC

Thu Feb 10 00:00:00 EST 2011

Int6 regulates both proteasomal degradation and translation initiation and is critical for proper formation of acini by human mammary epithelium.

Oncogene

Suo J,Snider SJ,Mills GB,Creighton CJ,Chen AC,Schiff R,Lloyd RE,Chang EC

Thu Feb 10 00:00:00 EST 2011

Int6 and Moe1 interact with Cdc48 to regulate ERAD and proper chromosome segregation.

Cell cycle (Georgetown, Tex.)

Otero JH,Suo J,Gordon C,Chang EC

Fri Jan 01 00:00:00 EST 2010

Int6 and Moe1 interact with Cdc48 to regulate ERAD and proper chromosome segregation.

Cell cycle (Georgetown, Tex.)

Otero JH,Suo J,Gordon C,Chang EC

Fri Jan 01 00:00:00 EST 2010

HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly.

The EMBO journal

Locker N,Easton LE,Lukavsky PJ

Wed Feb 07 00:00:00 EST 2007

HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly.

The EMBO journal

Locker N,Easton LE,Lukavsky PJ

Wed Feb 07 00:00:00 EST 2007

A practical approach to isolate 48S complexes: affinity purification and analyses.

Methods in enzymology

Locker N,Lukavsky PJ

Mon Dec 03 00:00:00 EST 2007

A practical approach to isolate 48S complexes: affinity purification and analyses.

Methods in enzymology

Locker N,Lukavsky PJ

Mon Dec 03 00:00:00 EST 2007

Affinity purification of eukaryotic 48S initiation complexes.

RNA (New York, N.Y.)

Locker N,Easton LE,Lukavsky PJ

Sat Apr 01 00:00:00 EST 2006

Affinity purification of eukaryotic 48S initiation complexes.

RNA (New York, N.Y.)

Locker N,Easton LE,Lukavsky PJ

Sat Apr 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: eIF-3-zeta; eIF3 p66; EIF3D; Eukaryotic translation initiation factor 3 subunit 7; Eukaryotic translation initiation factor 3 subunit D; eukaryotic translation initiation factor 3, subunit 7 (zeta); eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa; eukaryotic translation initiation factor 3, subunit D; RP5-1119A7.12-003; translation initiation factor eIF3 p66; translation initiation factor eIF3 p66 subunit

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Gene Aliases: 66/67kDa; AA407891; eIF3-p66; eIF3-zeta; EIF3D; eIF3p66; EIF3S7

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UniProt ID: (Human) O15371, (Mouse) O70194

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Entrez Gene ID: (Human) 8664, (Mouse) 55944

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Molecular Function: translation initiation factor

Function(s)

formation of translation preinitiation complex formation of cytoplasmic translation initiation complex translational initiation regulation of translational initiation IRES-dependent viral translational initiation viral translational termination-reinitiation translation

Process(es)

translation initiation factor activity protein binding poly(A) RNA binding

Performance Guarantee

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EIF3D Polyclonal Antibody

EIF3D Antibody (10219-1-AP) in ICC/IF

Product Details

10219-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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