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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 128 and 410 of Human Integrin beta 1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA5-29606 has successfully been used to detect CD29 in IF, IHC and WB in human and horse.
The PA5-29606 immunogen is recombinant fragment corresponding to a region within amino acids 128 and 410 of Human Integrin beta 1.
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Tumor bioengineering using a transglutaminase crosslinked hydrogel.
PA5-29606 was used in immunohistochemistry to develop a 3D culturing system to mimic the tumor microenvironment using transglutaminase-cross-linked gelatin gel (Col-Tgel)
|Fang JY,Tan SJ,Yang Z,Tayag C,Han B||PloS one (9:null)||2014|
CD29; FNRB; glycoprotein IIa; GPIIA; integrin beta 1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-BETA; VLAB
CD29; FNRB; GPIIA; ITGB1; MDF2; MSK12; VLA-BETA; VLAB