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Bethyl Laboratories
JMJD1A/JHDM2A Polyclonal Antibody
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FIGURE: 1 / 1
JMJD1A/JHDM2A Antibody (A301-539A) in WB
Detection of human JMJD1A/JHDM2A by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JMJD1A/JHDM2A antibody (Product # A301-539A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. JMJD1A/JHDM2A was also immunoprecipitated by rabbit anti-JMJD1A/JHDM2A antibody A301-538A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 se... View More
Product Details
A301-539A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1271 and 1321 of human jumonji domain containing 1A (JmjC domain-containing histone demethylation protein 2A).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [histone H3]-dimethyl-L-lysine(9) demethylase 3A; DKFZp686A24246; DKFZp686P07111; JmjC domain-containing histone demethylation protein 2A; jumonji C domain-containing histone demethylase 2A; Jumonji domain-containing protein 1A; lysine (K)-specific demethylase 3A; Lysine-specific demethylase 3A; testis-specific protein A; unnamed protein product
Gene Aliases: JHDM2A; JHMD2A; JMJD1; JMJD1A; KDM3A; KIAA0742; TSGA
UniProt ID: (Human) Q9Y4C1
Entrez Gene ID: (Human) 55818
chromatin binding
transcription cofactor activity
iron ion binding
protein binding
zinc ion binding
oxidoreductase activity
chromatin DNA binding
histone demethylase activity (H3-K9 specific)
metal ion binding
androgen receptor binding
dioxygenase activity
histone H3K9me/H3K9me2 demethylase activity
chromatin organization
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
spermatogenesis
spermatid nucleus elongation
hormone-mediated signaling pathway
regulation of gene expression
cell differentiation
androgen receptor signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
formaldehyde biosynthetic process
positive regulation of cold-induced thermogenesis
cellular response to leukemia inhibitory factor
regulation of stem cell population maintenance
regulation of stem cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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