Immunohistochemistry of Cytokeratin Pan Type II in Human breast tissue. Samples were incubated with Cytokeratin Pan Type II monoclonal antibody (Product # MA1-5762).

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Immunogen image

Immunogen Image Cytokeratin Pan Type II Monoclonal Antibody (AE3)

Product Details

MA1-5762

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

Product Specifications

Species Reactivity

Bovine, Chicken, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AE3

Immunogen

Human epidermal keratins from P3 x 63 Ag8 cells

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

200 µg

Purification

Protein G

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1075178

Product Specific Information

MA1-5762 reacts with all basic (type II) human keratins in human samples, including K1, K2, K3, K4, K5, K6, K7 and K8.

MA1-5762 has been successfully used in Western blot, immunohistochemistry and electron microscopy procedures.

The MA1-5762 immunogen is human epidermal keratins.

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 57kd keratin; 57kDa keratin; 58 kDa cytokeratin; 60-kDa keratin; 65 kDa cytokeratin; 67 kDa cytokeratin; alpha keratin; CK 2e; CK-1; CK-2e; CK-2P; CK-3; CK-4; CK-5; CK-6A; CK-6D; CK-7; CK-72; CK-8; cytokeratin 1; cytokeratin 2; cytokeratin 3; cytokeratin 4; cytokeratin 5; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; Cytokeratin 7; cytokeratin 8; Cytokeratin endo A; Cytokeratin otokeratin; cytokeratin type II, component Ia; cytokeratin type II, component Ib/c; cytokeratin type II, component III; Cytokeratin-1; Cytokeratin-2e; Cytokeratin-2P; Cytokeratin-3; Cytokeratin-4; Cytokeratin-5; Cytokeratin-6A; Cytokeratin-6D; Cytokeratin-7; Cytokeratin-72; Cytokeratin-8; Cytokeratin-A; Cytoskeletal 57 kDa keratin; cytoskeletal protein; ear specific cytokeratin; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolytic hyperkeratosis 1; Epithelial keratin-2e; Hair alpha protein; intermediate filament protein; K7; K1; K2e; K2P; K3; K3 keratin; K4; K5; K6A; K7; K72; K76; K8; KERA; keratin 1, type II; keratin 2 (epidermal ichthyosis bullosa of Siemens); keratin 2 epidermis; keratin 2, type II; keratin 2A; keratin 2p; keratin 3, type II; keratin 4, type II; keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 6 alpha; keratin 6, inner root sheath, 2; keratin 6A, , type II; keratin 6A, type II; keratin 7, type II; keratin 72, pseudogene; keratin 72, type II; keratin 76, type II; keratin 8, type II; keratin 86; keratin complex 2, basic, gene 1; keratin complex 2, basic, gene 17; keratin complex 2, basic, gene 35; keratin complex 2, basic, gene 4; keratin complex 2, basic, gene 5; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6c; keratin complex 2, basic, gene 7; keratin complex 2, basic, gene 8; keratin complex 2, gene 17; keratin complex 2, gene 6a; keratin IV; keratin K3; keratin K5; keratin protein K6irs; keratin type II; keratin, 55K type II cytoskeletal; keratin, epidermal type II, K6A; keratin, simple epithelial type I, K7; Keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 2 epidermal; Keratin, type II cytoskeletal 2 oral; Keratin, type II cytoskeletal 3; Keratin, type II cytoskeletal 4; Keratin, type II cytoskeletal 5; keratin, type II cytoskeletal 59 kDa, component IV; keratin, type II cytoskeletal 60 kDa, component III; Keratin, type II cytoskeletal 6A; Keratin, type II cytoskeletal 7; Keratin, type II cytoskeletal 72; Keratin, type II cytoskeletal 8; Keratin, type II cytoskeletal cochleal; Keratin-1; Keratin-2 epidermis; Keratin-2e; Keratin-3; Keratin-4; Keratin-5; Keratin-6-alpha; Keratin-6A; Keratin-7; Keratin-72; Keratin-76; Keratin-8; krt1 {ECO:0000250|UniProtKB:P04264}; krt2; krt2 {ECO:0000250|UniProtKB:P35908}; krt4 {ECO:0000250|UniProtKB:P19013}; KRT5; krt7; krt7 {ECO:0000250|UniProtKB:P08729}; krt76; MGC116967; MGC116968; mK6-alpha; otokeratin; pan keratin; Sarcolectin; tuftelin interacting protein 8; type II alpha-keratin IIA; type II alpha-keratin IIC; type II cytokeratin A (no.8); type II cytoskeletal cochleal; Type II inner root sheath-specific keratin-K6irs2; type II intermediate filament protein; type II keratin Kb1; type II keratin Kb2; type II keratin Kb35; type II keratin Kb4; type II keratin Kb7; type II keratin Kb9; type II keratin subunit protein; type II mesothelial keratin K7; Type-II keratin Kb1; Type-II keratin Kb2; Type-II keratin Kb3; Type-II keratin Kb35; Type-II keratin Kb4; Type-II keratin Kb5; Type-II keratin Kb6; Type-II keratin Kb7; Type-II keratin Kb8; Type-II keratin Kb9; type-II keratin-35; unnamed protein product

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Gene Aliases: 2310001L23Rik; 2310016L08Rik; 3300001P10Rik; AA960620; AEI2; AI507495; AL022697; AU019895; AW108092; AW146334; BB005427; CARD2; CK-2e; CK-3; CK-4; CK-6C; CK-6E; CK-72; CK-8; CK1; CK3; CK4; CK5; CK6A; CK6C; CK6D; CK7; CK8; CYK4; CYK8; CYKER; D15Wsu77e; DDD; DDD1; EBS1; EBS2; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; EHK; EHK1; EndoA; EPPK; HUMCYT2A; K1; K2C7; K2C8; K2e; K3; K4; K5; K6A; K6C; K6D; K6irs; K6IRS2; K7; K72; K8; Kb1; Kb2; KB35; Kb4; Kb7; Kb9; Ker2; keratin; KERIA; KERIB; KO; Krt-2.1; Krt-2.4; Krt-2.8; KRT1; KRT1A; KRT2; Krt2-1; Krt2-17; Krt2-2; Krt2-35; Krt2-4; Krt2-5; Krt2-6; Krt2-6a; Krt2-6c; Krt2-7; KRT2-8; KRT2A; KRT2B; KRT2E; KRT2P; KRT3; KRT4; KRT5; KRT5A; KRT6; KRT6A; KRT6C; KRT6D; KRT6IRS2; KRT7; KRT72; Krt72-ps; KRT76; KRT8; Krt86; KRTA; KRTE; MECD2; mK6[a]; MK6a; NEPPK; PC3; Sak57; SCL; Tfip8; WSN1

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Entrez Gene ID: (Rabbit) 100353415, (Pig) 100049664, (Human) 3848, (Rabbit) 100353162, (Pig) 100049665, (Human) 3849, (Bovine) 407188, (Rabbit) 100353668, (Human) 3850, (Pig) 100157304, (Bovine) 527819, (Pig) 100157675, (Human) 3851, (Rabbit) 100348388, (Pig) 100511564, (Bovine) 281268, (Chicken) 407779, (Rabbit) 100352658, (Human) 3852, (Bovine) 614456, (Human) 3853, (Pig) 102164968, (Chicken) 408041, (Human) 3855, (Chicken) 395772, (Rabbit) 100345579, (Bovine) 535697, (Bovine) 531057, (Human) 140807, (Bovine) 407189, (Human) 51350, (Rabbit) 100348891, (Bovine) 281269, (Chicken) 426896, (Pig) 100152077, (Human) 3856, (Rat) 300250, (Mouse) 16678, (Mouse) 16681, (Rat) 406228, (Mouse) 16682, (Rat) 315323, (Rat) 369017, (Mouse) 110308, (Mouse) 16687, (Mouse) 110310, (Rat) 300242, (Mouse) 105866, (Rat) 406227, (Rat) 407757, (Mouse) 77055, (Rat) 25626, (Mouse) 16691

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Function(s)

protein binding carbohydrate binding structural constituent of epidermis signaling receptor activity protein heterodimerization activity structural constituent of cytoskeleton cytoskeletal protein binding scaffold protein binding molecular_function structural molecule activity protein complex binding

Process(es)

keratinization intermediate filament organization complement activation, lectin pathway retina homeostasis biological_process negative regulation of inflammatory response establishment of skin barrier epithelial cell differentiation negative regulation of epithelial cell proliferation morphogenesis of an epithelium positive regulation of cell proliferation cell differentiation killing of cells of other organism wound healing defense response to Gram-positive bacterium antimicrobial humoral immune response mediated by antimicrobial peptide negative regulation of entry of bacterium into host cell intermediate filament-based process cytoskeleton organization embryo development epidermis development sebaceous gland development hair follicle morphogenesis keratinocyte activation keratinocyte proliferation keratinocyte migration tumor necrosis factor-mediated signaling pathway sarcomere organization response to hydrostatic pressure response to other organism cell differentiation involved in embryonic placenta development extrinsic apoptotic signaling pathway hepatocyte apoptotic process cell morphogenesis involved in differentiation multicellular organism development Wnt signaling pathway response to mechanical stimulus regulation of cell migration regulation of protein localization intermediate filament polymerization pigmentation response to oxidative stress peptide cross-linking fibrinolysis regulation of angiogenesis protein heterotetramerization keratinocyte development positive regulation of epidermis development intermediate filament cytoskeleton organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cytokeratin Pan Type II Monoclonal Antibody (AE3)

Cytokeratin Pan Type II Antibody (MA1-5762) in IHC (P)

Product Details

MA1-5762

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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