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Invitrogen
T-Lymphocytes Monoclonal Antibody (KEN-5), FITC
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Product Details
MA1-80464
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
KEN-5
Immunogen
Rabbit thymocytes.
Conjugate
FITC
FITC
FITC
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This clone recognizes rabbit (lapine) T-cells, including >90% thymocytes and approximately 40-45% mesenteric lymph node cells. It immunoprecipitates a 67 kDa protein from rabbit thymocytes. This antibody does not bind to rabbit CD5 transfectants and shows very limited binding to rabbit B-cells, which in adult rabbits have been demonstrated to express CD5. This clone may recognize an epitope that is dependant on glycosylation to maintain its structural configuration, which may explain why the antibody does not recognize recombinant rabbit CD5 produced in insect cells (which contain different glycans from those found in mammalian cells).
For flow cytometry, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Target Information
T Lymphocytes are a subtype of white blood cell that is central to cell-mediated immunity. T Lymphocytes mature in the thymus from thymocytes. The majority of human T cells rearrange their alpha and beta chains on the cell receptor and are termed alpha beta T cells (alpha/beta T cells) and are part of the adaptive immune system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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