Immunofluorescence analysis of LCK was performed using 70% confluent log phase Jurkat and THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with LCK Monoclonal Antibody (3A5) (Product # MA5-12303) at 1:100 in 0.1% BSA, incubated at 4-degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # ... View More

LCK Antibody in Immunoprecipitation (IP)

Product Image Gallery

LCK Antibody — Lck Protein Schematic-Lck is a 56 kDa, membrane-associated, non-receptor tyrosine kinase belonging to the Src family that plays a critical role in T-cell antigen receptor (TCR) signaling, inducing T-lymphocyte development, activation, proliferation and survival. Lck has been implicated in various diseases including cancer development and metastasis, viral infections relating to HIV, heart disease, and osteoclast-mediated bone resorption. Lck is regulated by phosphorylation on multiple residues including Ser-194 in the SH2 domain, Tyr-394 (autophosphorylation site) within the activation loop of the catalytic domain, and Tyr-505 (inhibitory site) near the C-terminus. TCR requires Lck for thymocyte differentiation and activation. Additionally, Lck is implicated in JAK and STAT signaling, which are constitutively activated in cells overexpressing Lck.

Immunogen image

Immunogen Image LCK Monoclonal Antibody (3A5)

Product Details

MA5-12303

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

Immunocytochemistry (ICC/IF)

1:20-1:100

Flow Cytometry (Flow)

1 µg/test

Immunoprecipitation (IP)

2 µg/mL

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

3A5

Immunogen

Recombinant protein corresponding to aa 1-225 of murine p56lck protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

100 µg

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10986446

Product Specific Information

MA5-12303 targets LCK (p56lck) in IP, ICC/IF, FACS and WB applications and shows reactivity with Human, mouse, and Rat samples.

The MA5-12303 immunogen is recombinant protein corresponding to aa 1-225 of mouse p56lck protein.

Target Information

LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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LCK Monoclonal Antibody (3A5)

LCK Antibody (MA5-12303) in ICC/IF