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Invitrogen
CD45R (B220) Monoclonal Antibody (YKIX753.22.2)
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FIGURE: 1 / 1
CD45R (B220) Antibody (MA5-28396) in Flow
Flow cytometry of CD45R (B220) in red cell lysed canine blood. Sample was incubated with CD45R (B220) monoclonal antibody (Product # MA5-28396) and gated on mononuclear cells. Figures are arranged as follows: A) Alexa Fluor 488 conjugated Rat anti Canine CD44 and RPE conjugated Rat IgG2a isotype control. B) Alexa Fluor 488 conjugated Rat anti Canine CD44 and RPE conjugated Rat anti Canine CD45R.
Product Details
MA5-28396
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
YKIX753.22.2
Immunogen
Canine thymocytes
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes canine CD45R and was clustered at the First Canine Leucocyte Antigen Workshop (CLAW). It precipitates an antigen of 220 kDa from Con A Blasts.
Target Information
CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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