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TEK Recombinant Rabbit Monoclonal Antibody (116)
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FIGURE: 1 / 2
TEK Antibody (MA5-29596) in ICC/IF
Immunofluorescence staining of Human Tie2 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with TEK Recombinant Rabbit Monoclonal Antibody (116) (Product # MA5-29596, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-29596
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
116
Immunogen
Recombinant Human Tie2/CD202b/TEK protein (Met1-Lys745)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human Tie2/CD202b/TEK.
Target Information
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD202b antigen; EC 2.7.10; EC 2.7.10.1; endothelial tyrosine kinase; TEK tyrosine kinase, endothelial; TEKT1; Tektin-1; tunica interna endothelial cell kinase; tyrosine kinase with Ig and EGF homology domains-2; tyrosine-protein kinase receptor TEK; tyrosine-protein kinase receptor TIE-2; unnamed protein product
Gene Aliases: CD202B; GLC3E; TIE-2; TIE2; VMCM; VMCM1
UniProt ID: (Human) Q02763
Entrez Gene ID: (Human) 7010
angiogenesis
endothelial cell proliferation
regulation of endothelial cell proliferation
positive regulation of endothelial cell proliferation
sprouting angiogenesis
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
cell-cell signaling
heart development
positive regulation of signal transduction
positive regulation of endothelial cell migration
negative regulation of angiogenesis
hemopoiesis
regulation of cell migration
cell-substrate adhesion
regulation of establishment or maintenance of cell polarity
substrate adhesion-dependent cell spreading
positive regulation of Rac protein signal transduction
positive regulation of Rho protein signal transduction
negative regulation of apoptotic process
regulation of vascular permeability
positive regulation of MAPK cascade
regulation of angiogenesis
positive regulation of angiogenesis
positive regulation of cell adhesion
Tie signaling pathway
negative regulation of inflammatory response
positive regulation of multicellular organismal process
positive regulation of focal adhesion assembly
positive regulation of protein kinase B signaling
definitive hemopoiesis
heart trabecula formation
positive regulation of ERK1 and ERK2 cascade
cellular response to mechanical stimulus
glomerulus vasculature development
regulation of intracellular signal transduction
positive regulation of intracellular signal transduction
regulation of endothelial cell apoptotic process
negative regulation of endothelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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