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Invitrogen
GPX4 Recombinant Rabbit Monoclonal Antibody (JU11-31)
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FIGURE: 1 / 17
GPX4 Antibody (MA5-32827) in ICC/IF
Immunocytochemistry analysis of GPX4 with PC-3M cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37°C, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum (30 min, room temp), incubated with recombinant monoclonal GPX4 (Product # MA5-32827) at a dilution of 1:50 (in 2% negative goat serum overnight at 4°C), followed by Alexa Fluor 488 conjugate Goat anti-Rabbit IgG at 1:1,000 dilution (PBS used in control). DAPI was used to stain the cell nuclei (blue).
Product Details
MA5-32827
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Zebrafish
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JU11-31
Immunogen
Synthetic peptide within Human GPX4 aa 23-72.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; Glutathione peroxidase 4; GPx-4; HGNC:4556; PHGPx; phospholipid hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase GPX4; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxide glutathione peroxidase, nuclear; selenoprotein GPX4; sperm nuclei glutathione peroxidase; sperm nucleus glutathione peroxidase
Gene Aliases: GPx-4; GPX4; GSHPx-4; MCSP; mtPHGPx; PHGPx; SMDS; snGPx; snPHGPx
UniProt ID: (Human) P36969, (Mouse) O70325, (Rat) P36970
Entrez Gene ID: (Human) 2879, (Mouse) 625249, (Rat) 29328
chromatin organization
lipid metabolic process
phospholipid metabolic process
apoptotic process
response to oxidative stress
spermatogenesis
dendrite development
arachidonic acid metabolic process
lipoxygenase pathway
cerebellum development
response to estradiol
response to lipopolysaccharide
multicellular organism growth
long-chain fatty acid biosynthetic process
protein polymerization
cellular oxidant detoxification
negative regulation of ferroptosis
glutathione metabolic process
multicellular organism development
hydrogen peroxide catabolic process
regulation of inflammatory response
oxidation-reduction process
aging
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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