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Invitrogen

HSF1 Monoclonal Antibody (10H8), APC

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HSF1 Monoclonal Antibody (10H8), APC

Product Details

MA5-45127

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:1,000
-

Immunocytochemistry (ICC/IF)

1:200
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-

Gel Shift (EMSA)

Assay-dependent
-
Product Specifications

Species Reactivity

Bovine, Guinea pig, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

10H8

Immunogen

Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395
View immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein G

Storage buffer

95.46mM phosphate/2.48mM MES, pH 7.2, with 2mM EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931581

Product Specific Information

1 µg/mL of MA5-45127 was sufficient for detection of HSF1 in 20 µg of heat shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody|Detects approximately 85kDa (unstressed cell lysates), andapproximately 95kDa (heat shocked cell lysates).

Target Information

All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: heat shock factor 1; heat shock factor 2; Heat shock factor protein 1; Heat shock transcription factor 1; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 gammaalpha isoform; heat shock transcription factor 1 gammabeta isoform; HSF 1; HSF1; Hsf1gammaalpha; Hsf1gammabeta; HSTF 1; unnamed protein product

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Gene Aliases: AA960185; HSF; HSF1; Hsf1alpha; Hsf1beta; HSTF1

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UniProt ID: (Bovine) Q08DJ8, (Human) Q00613, (Mouse) P38532

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Entrez Gene ID: (Bovine) 506235, (Rabbit) 100350887, (Human) 3297, (Chinese hamster) 100762942, (Rat) 79245, (Guinea pig) 101787582, (Mouse) 15499

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Function(s)
DNA binding heat shock protein binding chromatin DNA binding identical protein binding sequence-specific DNA binding protein self-association protein heterodimerization activity Hsp90 protein binding translation elongation factor binding promoter-specific chromatin binding transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding protein kinase binding STAT family protein binding sequence-specific single stranded DNA binding general transcription initiation factor binding sequence-specific double-stranded DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding winged helix/forkhead transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter MAPK cascade DNA repair mRNA processing positive regulation of cell proliferation negative regulation of protein complex assembly cellular response to heat cellular response to unfolded protein cellular macromolecular complex assembly positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter mRNA transport positive regulation of transcription from RNA polymerase II promoter in response to heat stress cellular response to cadmium ion cellular response to copper ion cellular response to gamma radiation cellular response to diamide regulation of cellular response to heat positive regulation of mRNA polyadenylation cellular response to sodium arsenite negative regulation of double-strand break repair via nonhomologous end joining in utero embryonic development embryonic placenta development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter defense response cellular response to DNA damage stimulus female meiotic division spermatogenesis response to nutrient cell proliferation response to heat positive regulation of gene expression negative regulation of gene expression negative regulation of cardiac muscle cell apoptotic process response to activity response to estradiol response to lipopolysaccharide negative regulation of tumor necrosis factor production response to testosterone cellular response to potassium ion positive regulation of multicellular organism growth response to amino acid positive regulation of macrophage differentiation epithelial cell proliferation negative regulation of epithelial cell proliferation embryonic process involved in female pregnancy positive regulation of stress granule assembly protein-containing complex assembly cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to estradiol stimulus cellular response to xenobiotic stimulus cellular response to radiation negative regulation of inclusion body assembly positive regulation of inclusion body assembly positive regulation of cold-induced thermogenesis response to peptide positive regulation of apoptotic DNA fragmentation cellular response to angiotensin cellular response to nitroglycerin cellular response to L-glutamine response to hypobaric hypoxia response to psychosocial stress positive regulation of apoptotic signaling pathway transcription, DNA-templated protein phosphorylation negative regulation of cell proliferation mRNA transcription transcription from RNA polymerase II promoter regulation of spindle checkpoint
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