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Invitrogen
MFN2 Monoclonal Antibody (N153/5), PE
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FIGURE: 1 / 3
MFN2 Antibody (MA5-45672) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Mitofusin 2 monoclonal antibody (Product # MA5-45672) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45672
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
N153/5
Immunogen
Fusion protein amino acids 370-600 (cytoplasmic N-terminus) of mouse Mitofusin-2.
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Rat: 97% identity (226/231 amino acids identical). Human: 92% identity (214/231 amino acids identical) ~55% identity with Mitofusin-1.
1 µg/mL of MA5-45672 was sufficient for detection of Mitofusin-2 in 20 µg of mouse cardiac mitochondrial lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 90kDa. No cross-reactivity against Mitofusin-1.
This antibody was formerly sold as clone S153-5.
Target Information
This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CPRP 1; HRP1; HSG protein; hyperplasia suppressor; hypertension related protein 1; hypertension-related protein; MARF; GTP-binding transmembrane protein; MARF; mitochondrial GTP-binding transmembrane protein; MFN 2; mitochondrial assembly regulatory factor; mitochondrial transmembrane GTPase FZO1A; mitofusin-2-like; Mitofusin2; related to proliferation of VSMCs; transmembrane GTPase MFN2; unnamed protein product
Gene Aliases: CMT2A; CMT2A2; CMT2A2A; CMT2A2B; CPRP1; D630023P19Rik; Fzo; HMSN6A; HSG; MARF; MSL
UniProt ID: (Human) O95140, (Mouse) Q80U63
Entrez Gene ID: (Human) 9927, (Rat) 64476, (Mouse) 170731
blastocyst formation
mitochondrion organization
mitochondrial membrane organization
spermatogenesis
mitochondrial fusion
negative regulation of cell proliferation
gonad development
male gonad development
aerobic respiration
response to manganese ion
positive regulation of gene expression
negative regulation of cardiac muscle cell apoptotic process
positive regulation of hydrogen peroxide biosynthetic process
positive regulation of mitochondrial membrane potential
response to muscle activity
response to nutrient levels
response to testosterone
protein localization to pre-autophagosomal structure
negative regulation of cell cycle
negative regulation of cell size
positive regulation of glucose import
negative regulation of Ras protein signal transduction
positive regulation of insulin receptor signaling pathway
response to cadmium ion
intracellular distribution of mitochondria
camera-type eye morphogenesis
negative regulation of smooth muscle cell proliferation
response to axon injury
mitochondrial calcium ion homeostasis
response to calcium ion
response to electrical stimulus
mitochondrion localization
negative regulation of glial cell proliferation
negative regulation of sarcomere organization
positive regulation of dendritic spine morphogenesis
parkin-mediated mitophagy in response to mitochondrial depolarization
response to fatty acid
cellular response to manganese ion
cellular response to glucose stimulus
cellular response to testosterone stimulus
cellular response to low-density lipoprotein particle stimulus
cellular response to hypoxia
cellular response to ionizing radiation
cellular response to dexamethasone stimulus
cellular response to mercaptoethanol
negative regulation of release of cytochrome c from mitochondria
negative regulation of mitochondrial fission
positive regulation of cold-induced thermogenesis
positive regulation of cellular respiration
negative regulation of reactive oxygen species biosynthetic process
positive regulation of reactive oxygen species biosynthetic process
response to L-arginine
negative regulation of vascular smooth muscle cell proliferation
positive regulation of vascular smooth muscle cell proliferation
cellular response to L-glutamate
response to D-galactose
response to flavonoid
positive regulation of vascular associated smooth muscle cell apoptotic process
response to hypobaric hypoxia
positive regulation of ovarian follicle development
positive regulation of estradiol secretion
positive regulation of progesterone secretion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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