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Invitrogen
Citrulline Monoclonal Antibody (6C2.1), APC
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FIGURE: 1 / 2
Citrulline Antibody (MA5-45757) in IHC (P)
Immunohistochemistry analysis using Colon tissue. Fixation involved formalin fixed, paraffin embedded treatment. Samples were incubated with Citrulline monoclonal antibody (Product # MA5-45757) at 1:25 for 1 hour at RT, followed by Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain used was DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin s... View More
Product Details
MA5-45757
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
6C2.1
Immunogen
Synthetic L-Citrulline conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:1,000 dilution of MA5-45757 was sufficient for detection of Citrulline in 2 µg of Citrulline conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for citrulline residues on modified proteins. Tested using citrulline conjugated Bovine Serum Albumin (BSA).
Target Information
Citrulline is an amino acid that is not incorporated into proteins during synthesis but is generated within proteins by the deimination of arginine. Citrulline occurs normally in many proteins, such as Myelin basic protein (MBP) while others, such as fibrin become citrullinated during inflammation or apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACPA; Citrulline; L-Citrulline
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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