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Invitrogen
CUGBP1 Recombinant Mouse Monoclonal Antibody (7A1-R)
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FIGURE: 1 / 8
CUGBP1 Antibody (MA5-56403) in ICC/IF
Immunocytochemistry analysis of CUGBP1 with HeLa cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp). Next they were incubated with CUGBP1 Recombinant Monoclonal Antibody (Product # MA5-56403)at a dilution of 1:100 in 1% BSA in PBST overnight at 4 &(8)451;. Followed by Goat Anti-Mouse IgG H&L at a dilution of 1:1,000. DAPI was used to stain the cell nuclei (blue).
Product Details
MA5-56403
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7A1-R
Immunogen
Recombinant protein within Human CELF1 aa 1-200/486.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG) n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis. HeLa cell protein, CUG-BP1, has been purified based upon its ability to bind specifically to (CUG) 8 oligonucleotides in vitro. CUG-BP1 is the major (CUG) 8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. The CUG-BP/hNab50 protein is localized predominantly in the nucleus and is associated with polyadenylated RNAs in vivo. In vitro RNA-binding/photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3-UTR. The (CUG) n repeat region in Mt-PK mRNA is a binding site for CUG-BP/hNab50 in vivo, and triplet repeat expansion leads to sequestration of this hnRNP on mutant Mt-PK transcripts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 50 kDa nuclear polyadenylated RNA-binding protein; Brain protein F41; bruno-like 2; Bruno-like protein 2; CELF-1; CUG RNA-binding protein; CUG triplet repeat RNA-binding protein 1; CUG triplet repeat, RNA binding protein 1; CUG-BP- and ETR-3-like factor 1; CUG-BP1; CUGBP Ela; CUGBP Elav-like family member 1; Deadenylation factor CUG-BP; Deadenylation factor EDEN-BP; EDEN-BP homolog; embryo deadenylation element binding protein; Embryo deadenylation element-binding protein homolog; nuclear polyadenylated RNA-binding protein, 50-kD; RNA-binding protein; RNA-binding protein BRUNOL-2; RP23-147D3.7; unnamed protein product
Gene Aliases: 1600010O03Rik; AA407467; BRUNOL2; CELF1; CUG-BP; CUG-BP1; CUGBP; CUGBP1; D2Wsu101e; EDEN-BP; HNAB50; NAB50; NAPOR
UniProt ID: (Human) Q92879, (Mouse) P28659, (Rat) Q4QQT3
Entrez Gene ID: (Human) 10658, (Mouse) 13046, (Rat) 362160
regulation of alternative mRNA splicing, via spliceosome
mRNA splice site selection
mRNA processing
germ cell development
negative regulation of cell proliferation
RNA splicing
embryo development ending in birth or egg hatching
positive regulation of gene expression
negative regulation of gene expression
posttranscriptional gene silencing
negative regulation of translation
posttranscriptional gene silencing by RNA
regulation of RNA splicing
regulation of inflammatory response
mRNA destabilization
spermatid development
positive regulation of multicellular organism growth
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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