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Invitrogen

EGFRvIII Recombinant Rabbit Monoclonal Antibody (RM419), Biotin

View all (5) EGFRvIII antibodies

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Product Details

MA562099

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200
-

Flow Cytometry (Flow)

1:10-1:100
-

ELISA (ELISA)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM419

Immunogen

A peptide corresponding to epidermal growth factor receptor variant III (EGFRvIII).

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

Conc. Not Determined

Purification

Protein A

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3703103

Product Specific Information

This antibody reacts to human EGFRvIII.

Target Information

EGFRvIII is included in receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; contains extracellular domain; EGFR vIII; Epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; erb-b2 receptor tyrosine kinase 1; GIG40; membrane protein; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; unnamed protein product

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Gene Aliases: EGFR; ERBB; ERBB1; ERRP; HER1; mENA; NISBD2; NNCIS; PIG61

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UniProt ID: (Human) P00533

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Entrez Gene ID: (Human) 1956

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Function(s)
nucleotide binding virus receptor activity chromatin binding double-stranded DNA binding protein kinase activity MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity protein binding ATP binding kinase activity transferase activity enzyme binding kinase binding protein phosphatase binding protein tyrosine kinase activator activity ubiquitin protein ligase binding identical protein binding cadherin binding epidermal growth factor binding actin filament binding ATPase binding
Process(es)
MAPK cascade cell morphogenesis ossification embryonic placenta development positive regulation of protein phosphorylation hair follicle development signal transduction cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway epidermal growth factor receptor signaling pathway salivary gland morphogenesis learning or memory cell proliferation positive regulation of cell proliferation epidermis development cerebral cortex cell migration neuron differentiation positive regulation of cell growth positive regulation of cell migration positive regulation of peptidyl-serine phosphorylation ERBB2-EGFR signaling pathway negative regulation of epidermal growth factor receptor signaling pathway regulation of cell proliferation negative regulation of protein catabolic process positive regulation of phosphorylation negative regulation of apoptotic process positive regulation of MAPK cascade protein kinase B signaling positive regulation of DNA repair positive regulation of DNA replication positive regulation of transcription from RNA polymerase II promoter viral entry into host cell positive regulation of fibroblast proliferation digestive tract morphogenesis epithelial cell proliferation positive regulation of epithelial cell proliferation regulation of peptidyl-tyrosine phosphorylation protein insertion into membrane positive regulation of protein kinase B signaling morphogenesis of an epithelial fold eyelid development in camera-type eye response to UV-A positive regulation of ERK1 and ERK2 cascade cellular response to amino acid stimulus cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to estradiol stimulus positive regulation of protein kinase C signaling positive regulation of canonical Wnt signaling pathway cell-cell adhesion positive regulation of G1/S transition of mitotic cell cycle positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of protein localization to plasma membrane negative regulation of cardiocyte differentiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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