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N-Ras Polyclonal Antibody
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FIGURE: 1 / 4
N-Ras Antibody (PA5-115671) in IHC (P)
Immunohistochemistry analysis of N-Ras in mouse kidney tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115671) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.
Product Details
PA5-115671
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NRAS(Accession P01111), corresponding to amino acid residues E153-M189.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total NRAS.
Target Information
NRAS (GTPase NRas) is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML) and Noonan syndrome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GTPase NRas; N-ras protein part 4; Neuroblastoma RAS; neuroblastoma RAS viral (v-ras) oncogene homolog; neuroblastoma RAS viral oncogene homolog; proto-oncogene GTPase; RAS; RASN; small GTP binding protein; Transforming protein N-Ras; unnamed protein product; v-ras neuroblastoma RAS viral oncogene homolog
Gene Aliases: ALPS4; AV095280; CMNS; HRAS1; KRAS; N-ras; NCMS; NRAS; NRAS1; NS6
UniProt ID: (Human) P01111, (Rat) Q04970
Entrez Gene ID: (Human) 4893, (Mouse) 18176, (Rat) 24605
MAPK cascade
positive regulation of endothelial cell proliferation
signal transduction
Ras protein signal transduction
regulation of protein phosphorylation
small GTPase mediated signal transduction
regulation of gene expression
positive regulation of fibroblast proliferation
negative regulation of skeletal muscle tissue development
regulation of cell cycle
establishment of protein localization to Golgi
biological_process
positive regulation of cell proliferation
visual learning
actin cytoskeleton organization
regulation of synaptic transmission, GABAergic
positive regulation of Rac protein signal transduction
negative regulation of neuron apoptotic process
negative regulation of cell differentiation
regulation of long-term neuronal synaptic plasticity
striated muscle cell differentiation
epithelial tube branching involved in lung morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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