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Proteintech
NDUFS1 Polyclonal Antibody
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FIGURE: 1 / 14
NDUFS1 Antibody (12444-1-AP) in ICC/IF
Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells stained for NDUFS1 rabbit pAb (12444-1-AP) at dilution of 1:50 and alpha Tubulin mouse mAb (66031-1-IG) at dilution of 1:100, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) for 12444-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-mouse IGG (H+L) for 66031-1-IG.
Product Details
12444-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
NDUFS1 Fusion Protein Ag3135 (1-305 aa encoded by BC030833)
Conjugate
Form
Liquid
Concentration
0.17 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
Immunogen sequence: MLRIPVRKA LVGLSKSPKG CVRTTATAAS NLIEVFVDGQ SVMVEPGTTV LQACEKVGMQ IPRFCYHERL SVAGNCRMCL VEIEKAPKVV AACAMPVMKG WNILTNSEKS KKAREGVMEF LLANHPLDCP ICDQGGECDL QDQSMMFGND RSRFLEGKRA VEDKNIGPLV KTIMTRCIQC TRCIRFASEI AGVDDLGTTG RGNDMQVGTY IEKMFMSELS GNIIDICPVG ALTSKPYAFT AQPWETRKTE SIDVMDAVGS NIVVSTRTGE VMRILPRMHE DINEEWISDK TRFAYDGLKR QRLTEP (1-305 aa encoded by B C030833)
Target Information
The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CI-75kD; complex I 75kDa subunit; complex I, mitochondrial respiratory chain, 75-kD subunit; Complex I-75kD; MGC26839; mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit; NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa; NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase); NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; NDUFS-1; OTTHUMP00000206345
Gene Aliases: 5830412M15Rik; 9930026A05Rik; CI-75k; CI-75Kd; NDUFS1; PRO1304
UniProt ID: (Human) P28331, (Rat) Q66HF1, (Mouse) Q91VD9
Entrez Gene ID: (Human) 4719, (Rat) 301458, (Mouse) 227197
protein binding
NADH dehydrogenase (ubiquinone) activity
electron carrier activity
metal ion binding
2 iron, 2 sulfur cluster binding
4 iron, 4 sulfur cluster binding
molecular_function
NADH dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on NAD(P)H
iron-sulfur cluster binding
reductase
mitochondrial electron transport, NADH to ubiquinone
apoptotic mitochondrial changes
mitochondrial respiratory chain complex I assembly
cellular respiration
ATP metabolic process
regulation of mitochondrial membrane potential
reactive oxygen species metabolic process
biological_process
ATP synthesis coupled electron transport
transport
oxidation-reduction process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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