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Invitrogen
USP16 Polyclonal Antibody
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FIGURE: 1 / 7
USP16 Antibody (PA5-104195) in ICC/IF
Immunofluorescent analysis of USP16 in HepG2. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with USP16 polyclonal antibody (Product # PA5-104195) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Product Details
PA5-104195
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human USP16(Accession Q9Y5T5), corresponding to amino acid residues K140-P190.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total USP16.
Target Information
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 16; deubiquitinating enzyme 16 {ECO:0000255|HAMAP-Rule:MF_03062}; deubiquitinating enzyme homolog; Ubiquitin carboxyl-terminal hydrolase 16; ubiquitin carboxyl-terminal hydrolase 16 {ECO:0000255|HAMAP-Rule:MF_03062}; ubiquitin specific protease 16; Ubiquitin thioesterase 16; ubiquitin thioesterase 16 {ECO:0000255|HAMAP-Rule:MF_03062}; ubiquitin thiolesterase 16; Ubiquitin-processing protease UBP-M; ubiquitin-specific processing protease 16; Ubiquitin-specific-processing protease 16; ubiquitin-specific-processing protease 16 {ECO:0000255|HAMAP-Rule:MF_03062}; unnamed protein product
Gene Aliases: 1200004E02Rik; 2810483I07Rik; 6330514E22Rik; MSTP039; UBP-M; UBPM; USP16
UniProt ID: (Human) Q9Y5T5, (Rat) Q2KJ09, (Mouse) Q99LG0
Entrez Gene ID: (Human) 10600, (Rat) 288306, (Mouse) 74112
transcription coactivator activity
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
peptidase activity
cysteine-type peptidase activity
zinc ion binding
hydrolase activity
histone binding
ribosomal small subunit binding
ubiquitin binding
metal ion binding
histone H2A deubiquitinase activity
molecular_function
thiol-dependent ubiquitinyl hydrolase activity
cysteine protease
mitotic cell cycle
chromatin organization
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
proteolysis
cellular response to DNA damage stimulus
regulation of gene expression
protein deubiquitination
monoubiquitinated protein deubiquitination
positive regulation of transcription, DNA-templated
positive regulation of translational elongation
positive regulation of transcription from RNA polymerase II promoter
protein homotetramerization
cell division
regulation of cell cycle
positive regulation of ribosome biogenesis
mitotic nuclear division
transcription, DNA-templated
mitotic nuclear division
biological_process
histone deubiquitination
monoubiquitinated histone H2A deubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
histone H2A K63-linked deubiquitination
regulation of transcription, DNA-templated
ubiquitin-dependent protein catabolic process
cell cycle
chromatin modification
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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